Compounds that may selectively inhibit Oplophorus luciferase-derived bioluminescent complexes, e.g., NanoBiT® bioluminescent complex, are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

A method for generating a three-dimensional luminescence image includes setting a focal interval between two-dimensional images in accordance with localization of luminescence in a three-dimensional sample. The three-dimensional sample contains a plurality of cells prepared to be luminescent and has a three-dimensional shape. The two-dimensional images have mutually different focal planes and are acquired at the focal interval. The method further includes acquiring a two-dimensional image set including two-dimensional images at the focal interval that is set by imaging the three-dimensional sample under an unirradiated condition; and generating a three-dimensional luminescence image by combining the two-dimensional images included in the two-dimensional image set together.

An ultra-weak light multispectral imaging method and an ultra-weak light multispectral imaging system, which can realize multispectral two-dimensional imaging of an ultra-weak light object by constituting a linear array from single-photon detectors of all response wavelengths and combining it with light-splitting technology. The ultra-weak light multispectral two-dimensional imaging system realizes high-resolution optical modulation by adopting the compressive sensing (CS) theory and the digital light processing (DLP) technology and using a linear array single-photon detector as a detection element; the ultra-weak light multispectral two-dimensional imaging system comprises a light filter, a first lens (1), a DMD control system, a second lens, a spectrophotometer, a linear array single-photon detector consisting of a plurality of single-photon detectors with different response wavelengths, and a central processing unit; and the sensitivity of the system can reach the single-photon level. The invention can be widely applied in the fields of biological self-illumination, medical diagnosis, nondestructive material analysis, astronomical observation, national defense and military, spectral measurement, quantum electronics and the like.

Disclosed are methods and kits for analyzing a sample comprising 1,5-anhydroglucitol and a possible first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte. Also provided are kits including reagents for practicing the claimed methods.

According to one embodiment, a biosensor includes a substrate and a sensor matrix that is present in a two-dimensional region on the substrate. The sensor matrix includes a plurality of basic blocks. Each of the basic blocks includes at least three types of sensor elements.

Embodiments of the invention provide an improved biosensor for biological or chemical analysis. According to embodiments of the invention, backside illumination (BSI) complementary metal-oxide-semiconductor (CMOS) image sensors can be used to effectively analyze and measure fluorescence or chemiluminescence of a sample. This measured value can be used to help identify a sample. Embodiments of the invention also provide methods of manufacturing an improved biosensor for biological or chemical analysis and systems and methods of DNA sequencing.

Provided herein are imaging cassettes for detecting a luminescent and/or radioactive signals. Such cassettes are useful in common biological assays, e.g., immunoassays, nucleotide detection assays, and other affinity assays.

In a sample analyzing apparatus, an injector assembly injects a reagent onto a sample, and luminescent light from the sample is transmitted to a detector. The assembly may be movable toward and away from the sample. The assembly may include one or more needles that communicate with one or more reservoirs supplying reagent or other liquids. The assembly may include a light guide for communicating with the detector. A cartridge may be provided in which the assembly, one or more reservoirs, and one or more pumps are disposed. The cartridge and/or the apparatus may be configured for enabling rinsing or priming to be done outside the apparatus. The cartridge and/or the apparatus may include one or more types of sensors configured for detecting, for example, the presence of liquid or bubbles in one or more locations of the apparatus and/or the cartridge.

A chemical, biochemical or biological analysis system and method including: a surface acoustic wave (SAW) actuator (6) including a piezoelectric substrate (9) and at least one interdigital electrode (17) located on a working surface (11) of the piezoelectric substrate (9), the SAW actuator generating travelling SAWs in the working surface when an electrical signal is applied to the interdigital electrode; at least one reaction chamber (19) located on the working surface of the piezoelectric substrate; a light detector (16) for detecting luminescent, fluorescent or phosphorescent emissions within the reaction chamber; a reagent flow line (29) for providing a flow of reagent through the reaction chamber; a test sample supply line (29) for supplying a test sample to the reaction chamber; wherein the SAW actuator can generate travelling SAWs within the working surface to thereby induce chaotic micromixing, convective transport, concentration or combinations thereof of the test sample and the reagent contained within the reaction chamber.

An integrated microtomography and optical imaging system includes a rotating table that supports an imaging object, an optical stage, and separate optical and microtomography imaging systems. The table rotates the imaging object about a vertical axis running therethrough to a plurality of different rotational positions during a combined microtomography and optical imaging process. The optical stage can be a trans-illumination, epi-illumination or bioluminescent stage. The optical imaging system includes a camera positioned vertically above the imaging object. The microtomography system includes an x-ray source positioned horizontally with respect to the imaging object. Optical and x-ray images are both obtained while the imaging object remains in place on the rotating table. The stage and table are included within an imaging chamber, and all components are included within a portable cabinet. Multiple imaging objects can be imaged simultaneously, and side mirrors can provide side views of the object to the overhead camera.