Provided is a nanoplasmonic sensor and a kit for biomolecule analysis, and a method of analyzing a biomolecule using the same. The method includes: providing the nanoplasmonic sensor including a dielectric grating extending in one direction, and a metal structure disposed to cover an upper surface and a side surface of the dielectric grating and have at least one bent portion; immobilizing a first probe molecule on a surface of the metal structure; hybridizing an analyte with the first probe molecule by introducing the analyte having a base sequence complementary to the first probe molecule; binding a second probe molecule that is hybridized with the first probe molecule to the analyte; binding an enzyme to the second probe molecule; introducing a substrate that reacts with the enzyme to produce a precipitate by an enzymatic reaction; and measuring localized surface plasmon resonance in the metal structure.

Certain embodiments provide a marker for detecting a detection target substance, including an assay reagent having a medium, and a composition containing a solvent or a solvent mixture, and a fluorescent coagulation factor that has a fluorescence property that changes as it coagulates in presence of the detection target substance. A difference of a hydrophobicity parameter LogP between the solvent and a part or the entirety of the detection target substance is equal to or smaller than 1.53.

Certain embodiments provide a marker for detecting a detection target substance, including an assay reagent having a medium, and a composition containing a solvent or a solvent mixture, and a fluorescent coagulation factor that has a fluorescence property that changes as it coagulates in presence of the detection target substance. A difference of a hydrophobicity parameter LogP between the solvent and a part or the entirety of the detection target substance is equal to or smaller than 1.53.

A fluidic device for detecting a target molecule in a fluid sample comprising a blocking chamber in fluidic communication with a detection chamber forming a blocking-detection chamber pair, the blocking chamber provided with at least one reagent for binding a non-target molecule in the sample, the blocking chamber adapted to maintain at least a portion of the bound non-target molecule within the blocking chamber, the detection chamber comprising at least one reagent for binding a target-molecule such that the target-molecule may be detected. The device comprises a combination detection chamber adapted to receive at least one reagent for binding both target and non-target molecules such that the combination of target and non-target molecules may be detected by binding to a detector.

A fluidic device for detecting a target molecule in a fluid sample comprising a blocking chamber in fluidic communication with a detection chamber forming a blocking-detection chamber pair, the blocking chamber provided with at least one reagent for binding a non-target molecule in the sample, the blocking chamber adapted to maintain at least a portion of the bound non-target molecule within the blocking chamber, the detection chamber comprising at least one reagent for binding a target-molecule such that the target-molecule may be detected. The device comprises a combination detection chamber adapted to receive at least one reagent for binding both target and non-target molecules such that the combination of target and non-target molecules may be detected by binding to a detector.

A point-of-use kit is designed for optically detecting and quantifying bacterial endotoxin by employing specific formulations of Limulus amebocyte lysate (LAL), each formulation designed to optimize results with different sample classes. Kits are pre-certified for use with a variety of environmental, industrial, and clinical samples, each sample category having a unique kit design and containing a unique lysate reagent formulation. Pre-certification transfers time and reagent consuming tasks, such as assessment of sample compatibility and sample effect on reagent sensitivity to endotoxin from the user to the kit producer. A fixed dilution/sample treatment is employed, eliminating the need for a comparative water standard and for a sample positive control. The kit has LAL reagent prepackaged in dry polyethylene capped tubes, which retains reagent shelf life and optical clarity for accurate and reproducible results using a portable spectrophotometer/optical reader.

Provided is a flocculation state monitoring sensor with which blockage of an ejecting part which ejects a gas towards a light emitting part and a light receiving part can be prevented, and which performs stable monitoring. A flocculation state monitoring sensor comprising: a light emitting part which irradiates laser light towards a measuring region which measures a flocculation state; and a light receiving part which receives light scattered along a direction which intersects with a direction along an optical axis of said light emitting part, wherein the light emitting part and the light receiving part are cleaned by air being ejected from nozzles theretowards. A small amount of air is provided to the nozzles between cleaning periods to purge floc, etc.

Systems and methods to determine concentrations of certain polymer encapsulators in drilling fluids based on light scattering, turbidity analysis and other analysis techniques. The method generally includes disposing a sample of drilling fluid within a vessel. A precipitant is added to form a precipitate with the desired polymer resulting in turbidity which is then measured in a variety of ways to determine the amount of polymer in the drilling fluid.

Systems and methods to determine concentrations of certain polymer encapsulators in drilling fluids based on light scattering, turbidity analysis and other analysis techniques. The method generally includes disposing a sample of drilling fluid within a vessel. A precipitant is added to form a precipitate with the desired polymer resulting in turbidity which is then measured in a variety of ways to determine the amount of polymer in the drilling fluid.

A spatial gradient-based fluorometer featuring a signal processor or processing module configured to: receive signaling containing information about light reflected off fluorophores in a liquid and sensed by a linear sensor array having a length and rows and columns of optical elements; and determine corresponding signaling containing information about a fluorophore concentration of the liquid a fluorophore concentration of the liquid that depends on a spatial gradient of the light reflected and sensed along the length of the linear sensor array, based upon the signaling received.