The present invention discloses a method for product quality control and fingerprint detection of an epimedium brevicornu complex. The method uses high performance liquid chromatography, and can effectively realize the quality control of products containing traditional Chinese medicine components, and especially stable control of the quality of products containing a large quantity of non-traditional Chinese medicine components in formulas. Through step-by-step quality control, product quality fluctuation is reduced and stable quality is ensured. Meanwhile, the method is simple and convenient, does not need additional instruments and standards, saves the cost and is more conducive to actual production.

A first liquid raw material and a second liquid raw material are reacted with each other by a reactor of a reaction device, so that a reaction product is produced. The reaction product is analyzed by an analyzer. In the controller, the reference value is acquired by the reference value acquirer from the chromatogram obtained from the result of the analysis by the analyzer. An upper limit value and a lower limit value with respect to the reference value are set by an allowable range setter. At least one of a residence time of the first liquid raw material, a residence time of the second liquid raw material, a reaction temperature, and a reaction pressure in the reactor is dynamically changed as a control target by a reaction controller such that the reference value falls between the upper limit value and the lower limit value.

The present disclosure pertains to methods of separating nucleic acid component compounds from one another. In some embodiments, the methods comprise: (a) loading a sample fluid comprising a plurality of nucleic acid component compounds onto a chromatographic column comprising a zwitterionic stationary phase contained inside the column; (b) flowing a mobile phase through the chromatographic column over a time period thereby forming an eluent in which at least some of the plurality of the nucleic acid component compounds are separated from each other, the mobile phase comprising a polar aprotic solvent, a protic solvent, and a volatile buffer salt, wherein flowing the mobile phase comprises varying a ratio of the protic solvent to the polar aprotic solvent over at least a portion of the time period and varying an ionic strength of the volatile buffer salt over at least a portion of the time period.

An autosampler is switched selectively between an injecting mode where a sampling flow path is incorporated into an analysis flow path of a liquid chromatograph and a loading mode where the sampling flow path is not incorporated into the analysis flow path and injects a sample into the analysis flow path at a position farther upstream than a separation column by being switched to the injecting mode with the sample held in the sampling flow path, and includes a clog determiner configured to acquire a sending liquid pressure of a liquid sending pump that sends a mobile phase in the analysis flow path, obtain a variation value of the liquid sending pressure when the injecting mode and the loading mode are switched and determine presence or absence of a clog in a system incorporated into the analysis flow path in the injecting mode based on the obtained variation value.

An analysis assistance method includes setting pressure in a first BPR to a value higher than a prescribed second set value with pressure in a second BPR set to a second set value, instructing a supercritical fluid chromatograph to supply a mobile phase to a supply flow path at a flow rate of the mobile phase that is to be theoretically supplied to a first flow path when the mobile phase is supplied to the supply flow path at a prescribed total flow rate and a prescribed sample introduction ratio, and gradually decreasing a set value of the pressure in the first BPR, and detecting a set value of the pressure in the first BPR at the time when supply of the mobile phase to a second flow path is stopped due to a decrease in set value of the pressure in the first BPR, as a first set value.

Techniques and apparatus for executing jobs for performing analytical methods are described. In one embodiment, for example, an apparatus may include at least one memory, and logic coupled to the at least one memory. The logic may be configured to receive a job request from a data system to perform a job, and determine an acquisition system to perform the job, the acquisition system to determine at least one task for the job, provide the at least one task to a task sequencer to coordinate performance of the at least one task, and provide data artifacts to the data system resulting from performance of the at least one task. Other embodiments are described.

A sample injector for a chromatography system is configured for injecting a sample fluid into a mobile phase, and includes a needle and a conduit. The needle is configured for aspirating the sample fluid and includes a needle tip, a needle channel through the needle for guiding the aspirated sample fluid, and a needle opening at the needle tip into which the needle channel opens. The conduit is configured for fluidically coupling with the needle and includes a conduit tip, and a conduit channel through the conduit for guiding fluid and having a conduit opening at the conduit tip. The conduit tip and the needle tip are configured to be pressed against each other for fluidically coupling the conduit channel with the needle channel, with at least a portion of the conduit tip penetrating into the needle opening for providing the fluidic coupling between the conduit and needle channels.

The present invention relates to a separation method comprising: i) providing an aqueous solution comprising a target compound; ii) applying a separation step to the aqueous solution, thereby providing a plurality of fractions of the aqueous solution: iii) determining a concentration parameter indicating a concentration of the target compound in at least part of the fractions; iv) determining a nuclear magnetic resonance (NMR) parameter by applying an NMR measurement to the fractions, the NMR parameter indicating a nuclear magnetic spin relaxation in said at least part of the fractions; and v) determining a target parameter of said at least part of the fractions based on the concentration parameter and the nuclear magnetic resonance parameter. The present invention further relates to separation systems, uses, preparations, and methods related thereto.

This invention provides methods, reagents, and diagnostic and prognostic markers useful for minimally invasive identification, diagnosis, and therapeutic intervention in individuals with colorectal cancers, or individuals who may be susceptible to developing colorectal cancers.

Disclosed is a liquid chromatography system and mixer for use therein that includes a first split connected to an inlet, the first split branching the flow of fluid from the inlet into a first path and a second path; a second split connected to an outlet of the first path, the second split branching the first path into a third path and a fourth path; and a third split connected to an outlet of the second path, the third split branching the second path into a fifth path and a sixth path. The first path and the second path are offset by a first predetermined volume, the third path and the fourth path are offset by a second predetermined volume, and the fifth path and the sixth path are also offset by the second predetermined volume.