Provided is, for example, a method for analyzing steroid hormones contained in a body hair sample from an animal, wherein body hair collected from an animal is used as the body hair sample, and the method includes: an extraction step of extracting a plurality of types of steroid hormones from the body hair sample by using a 45 vol % to 55 vol % aqueous acetonitrile solution containing 0.1 M trifluoroacetic acid; and a measurement step of measuring the amounts of the plurality of types of steroid hormones extracted.

Provided is, for example, a method for analyzing steroid hormones contained in a body hair sample from an animal, wherein body hair collected from an animal is used as the body hair sample, and the method includes: an extraction step of extracting a plurality of types of steroid hormones from the body hair sample by using a 45 vol % to 55 vol % aqueous acetonitrile solution containing 0.1 M trifluoroacetic acid; and a measurement step of measuring the amounts of the plurality of types of steroid hormones extracted.

A gas sampler (30) is provided with a connection portion (C1) connectable to a sample tank (20), a sample loop (PL) for holding a sample gas introduced from the sample tank (20) to the connection portion (C1), pneumatic switching valves (V1 to V6) for switching a flow path connected to the sample loop (PL), a control piping (81) for transmitting a driving pressure to the switching valves (V1 to V6), a pump (31) for suctioning an inside of the sample loop (PL), and a pressure accumulation tank (80) for accumulating the pressure generated by the operation of the pump (31) as a source pressure.

A gas sampler (30) is provided with a connection portion (C1) connectable to a sample tank (20), a sample loop (PL) for holding a sample gas introduced from the sample tank (20) to the connection portion (C1), pneumatic switching valves (V1 to V6) for switching a flow path connected to the sample loop (PL), a control piping (81) for transmitting a driving pressure to the switching valves (V1 to V6), a pump (31) for suctioning an inside of the sample loop (PL), and a pressure accumulation tank (80) for accumulating the pressure generated by the operation of the pump (31) as a source pressure.

The present invention generally pertains to methods of quantifying free fatty acids in a pharmaceutical formulation. In particular, the present invention pertains to a method of quantifying free fatty acids released from polysorbate hydrolysis in a pharmaceutical formulation comprising a pharmaceutical product, polysorbate and free fatty acids, using liquid chromatography-mass spectrometry, without the use of an extraction step.

The present invention generally pertains to methods of quantifying free fatty acids in a pharmaceutical formulation. In particular, the present invention pertains to a method of quantifying free fatty acids released from polysorbate hydrolysis in a pharmaceutical formulation comprising a pharmaceutical product, polysorbate and free fatty acids, using liquid chromatography-mass spectrometry, without the use of an extraction step.

A method for identifying converters from tobacco seedling population. The method includes: 1) sowing and cultivating tobacco seeds to be identified for 45-55 days; sampling a plurality of leaf disks from each of 45-55 days old seedlings; 2) incubating the plurality of leaf disks of each seedling in a sealed container at 37° C. for 10-12 hours, thereby obtaining a plurality of incubated tobacco leaves of each seedling; 3) immersing the plurality of incubated tobacco leaves of each seedling in an extractant, extracting alkaloids and obtaining an extract of each seedling; 4) analyzing the amounts of nicotine and nornicotine in the alkaloids extract of each seedling; and 5) automatically recognizing peaks of the alkaloids extract of each seedling, and calculating the percent nicotine conversion (PNC) and the pseudo percent nicotine conversion (PPNC).

A method for identifying converters from tobacco seedling population. The method includes: 1) sowing and cultivating tobacco seeds to be identified for 45-55 days; sampling a plurality of leaf disks from each of 45-55 days old seedlings; 2) incubating the plurality of leaf disks of each seedling in a sealed container at 37° C. for 10-12 hours, thereby obtaining a plurality of incubated tobacco leaves of each seedling; 3) immersing the plurality of incubated tobacco leaves of each seedling in an extractant, extracting alkaloids and obtaining an extract of each seedling; 4) analyzing the amounts of nicotine and nornicotine in the alkaloids extract of each seedling; and 5) automatically recognizing peaks of the alkaloids extract of each seedling, and calculating the percent nicotine conversion (PNC) and the pseudo percent nicotine conversion (PPNC).

Disclosed is a detection method for N-nitrosodimethylamine (NDMA) impurities, comprising: (1) obtaining a test solution containing a sample to be tested; and (2) detecting the test solution by means of gas chromatography-mass spectrometry to determine the content of an N-nitrosodimethylamine impurity in the sample. The method provided in the present invention has a good separating effect, a wide linear range, a high sensitivity and a good method durability, and can detect the content of N-nitrosodimethylamine (NDMA) in the sample rapidly and effectively.

A impurity detection method of latamoxef sodium is configured to detect the impurities in latamoxef sodium for injection by using high performance liquid chromatography. A chromatographic condition is as follows: taking Agilent ZORBAX SB-CN cyano bonded silica gel column as a stationary phase, taking 0.005˜0.015 mol/L ammonium acetate solution-methanol as a mobile phase A; taking 0.02˜0.03 mol/L ammonium acetate solution-acetonitrile as a mobile phase B; adjusting a pH value of the ammonium acetate solution to 5.8˜6.5; the present disclosure has advantages of a simple operation, high column efficiency, a moderate tailing factor and a retention time of a main peak, high detection accuracy of strong alkali damage, effective separation between the main peak and each impurity peak without interference, which is conducive to accurately controlling quality of lyophilized powder of latamoxef sodium for injection and ensuring quality of drugs.