An injection valve switches between a first state in which a sample loop is connected to a separation flow path through which a mobile phase from a liquid sender flows and a second state in which the sample loop is disconnected from the separation flow path. A pump speed determiner, in a case where a sample intake operation by a syringe pump is started immediately after the injection valve is switched from the second state to the first state, and a filling operation of filling the sample loop with the sample by the syringe pump is started after the intake operation is completed, determines an intake operation speed of the syringe pump that is required in order for the filling operation to complete immediately before the injection valve is switched from the second state to the first state next time using a set injection interval time. The syringe pump operates at the intake operation speed determined by the pump speed determiner while performing the intake operation. The sample is injected by the injector at intervals of the set injection interval time.

A sample injection method for liquid chromatography is performed with an injection valve having a waste port, two sample loop ports, and two high-pressure ports. One high-pressure port can be connected to a pump and the other high-pressure port can be connected to a chromatography column. A sample loop is connected to one of the sample loop ports on one end and to a pump volume of a sample conveying device on the other end. A section of the sample loop can be separated to facilitate receiving a sample fluid in the sample loop. A control unit controls the injection valve and the sample conveying device. The sample injector allows a sample to be loaded into the sample loop and then pressurized to an operating pressure prior to injecting the sample into the chromatography column. The sample loop may also be isolated from the operating pressure for facilitating depressurization of the loop.

An online HPLC dissolution test system includes a dissolution tester and a liquid chromatograph. An autosampler of the liquid chromatograph includes at least one flow vial, a sampling needle and an injection port. The flow vial is connected to the dissolution tester via a pipe and is for storing a sample solution supplied from the dissolution tester therein. The sampling needle is for collecting the sample solution by sucking from the flow vial. The injection port is for injecting the sample solution from the sampling needle into the analysis channel. The controller of the liquid chromatograph includes an immediate analyzing execution part configured to cause the autosampler to execute immediate analyzing operation for sucking the sample solution in the flow vial with the sampling needle and directly injecting the sample solution into the injection port when the sample solution is supplied from the dissolution tester to the flow vial.

An online HPLC dissolution test system includes a dissolution tester and a liquid chromatograph. An autosampler of the liquid chromatograph includes at least one flow vial, a sampling needle and an injection port. The flow vial is connected to the dissolution tester via a pipe and is for storing a sample solution supplied from the dissolution tester therein. The sampling needle is for collecting the sample solution by sucking from the flow vial. The injection port is for injecting the sample solution from the sampling needle into the analysis channel. The controller of the liquid chromatograph includes an immediate analyzing execution part configured to cause the autosampler to execute immediate analyzing operation for sucking the sample solution in the flow vial with the sampling needle and directly injecting the sample solution into the injection port when the sample solution is supplied from the dissolution tester to the flow vial.

A sample injector for injecting a fluid into a fluidic path, wherein the sample injector comprises a robot arm configured for moving an injection needle, when being connected to the robot arm, between a fluid container containing the fluid and a seat in fluid communication with the fluidic path, the needle configured for aspirating the fluid from the fluid container, when the needle has been moved to the fluid container, and for injecting aspirated fluid into the fluidic path, when the needle is accommodated in the seat, and the seat configured for accommodating the needle and providing fluid communication with the fluidic path, wherein the robot arm is configured for selectively disconnecting the needle from the robot arm when the needle is accommodated in the seat, and wherein the robot arm is configured for performing a further task while the needle is disconnected from the robot arm.

A sample injector for injecting a fluid into a fluidic path, wherein the sample injector comprises a robot arm configured for moving an injection needle, when being connected to the robot arm, between a fluid container containing the fluid and a seat in fluid communication with the fluidic path, the needle configured for aspirating the fluid from the fluid container, when the needle has been moved to the fluid container, and for injecting aspirated fluid into the fluidic path, when the needle is accommodated in the seat, and the seat configured for accommodating the needle and providing fluid communication with the fluidic path, wherein the robot arm is configured for selectively disconnecting the needle from the robot arm when the needle is accommodated in the seat, and wherein the robot arm is configured for performing a further task while the needle is disconnected from the robot arm.

Provided is a preparative separation liquid chromatograph system and preparative separation condition searching method which allows for an easy setting of the preparative separation condition. A sample temporally separated into components by a separation column is introduced into a detector and a fraction collector, with each component fractionated and collected by the fraction collector based on the result of a detection by the detector. A controlling and processing unit holds the following data for each sample or compound in the form of a database: chromatogram data obtained when a liquid chromatograph analysis in a preparative separation condition searching mode is performed for various standard samples under a search condition; and chromatogram data obtained when a liquid chromatograph analysis in a preparative separation mode is performed under one or more sets of preparative separation conditions for the various standard samples, along with the preparative separation condition used in this analysis.

Provided is a preparative separation liquid chromatograph system and preparative separation condition searching method which allows for an easy setting of the preparative separation condition. A sample temporally separated into components by a separation column is introduced into a detector and a fraction collector, with each component fractionated and collected by the fraction collector based on the result of a detection by the detector. A controlling and processing unit holds the following data for each sample or compound in the form of a database: chromatogram data obtained when a liquid chromatograph analysis in a preparative separation condition searching mode is performed for various standard samples under a search condition; and chromatogram data obtained when a liquid chromatograph analysis in a preparative separation mode is performed under one or more sets of preparative separation conditions for the various standard samples, along with the preparative separation condition used in this analysis.

An injection needle cartridge for a sample injector for injecting a sample fluid into a mobile phase in a fluidic path of a fluid separation system between a mobile phase drive and a separation unit, the injection needle cartridge comprising an injection needle configured for aspirating the sample fluid from a fluid container when the injection needle has been moved to the fluid container, and for injecting aspirated sample fluid into the fluidic path when the injection needle is sealingly accommodated in a needle seat, and a sealing force generator configured for applying a sealing force to the injection needle for sealingly accommodating the injection needle in the needle seat, wherein the injection needle cartridge is configured for being substitutably mountable on a handling robot of the sample injector for handling the injection needle cartridge between the fluid container and the needle seat.

An injection needle cartridge for a sample injector for injecting a sample fluid into a mobile phase in a fluidic path of a fluid separation system between a mobile phase drive and a separation unit, the injection needle cartridge comprising an injection needle configured for aspirating the sample fluid from a fluid container when the injection needle has been moved to the fluid container, and for injecting aspirated sample fluid into the fluidic path when the injection needle is sealingly accommodated in a needle seat, and a sealing force generator configured for applying a sealing force to the injection needle for sealingly accommodating the injection needle in the needle seat, wherein the injection needle cartridge is configured for being substitutably mountable on a handling robot of the sample injector for handling the injection needle cartridge between the fluid container and the needle seat.