A flow cell, for detecting a fluidic sample separated by a sample separation apparatus, includes a cuvette, a flow channel formed at least partially in the cuvette and configured to enable a flow of the separated fluidic sample through the flow channel, an electromagnetic radiation inlet at which an excitation electromagnetic radiation beam is couplable into the cuvette, and an electromagnetic radiation outlet at which an emission electromagnetic radiation beam, generated by an interaction between the excitation electromagnetic radiation beam and the separated fluidic sample, is couplable out of the cuvette. A geometry of the cuvette is configured so that at least one point at the excitation backside surface of the cuvette is outside of a direct field of view of the electromagnetic radiation outlet.

A liquid chromatography system includes a solvent delivery system, a sample manager including a sample delivery system in fluidic communication with the solvent delivery system, the sample delivery system configured to inject a sample from a sample-vial into a chromatographic flow stream, a liquid chromatography column located downstream from the sample delivery system, and a detector located downstream from the liquid chromatography column. The sample delivery system further includes a first needle drive including a first sample needle configured to extract the sample from the sample-vial and deliver the sample to the liquid chromatography column, and a first syringe in communication with the first sample needle configured to meter extraction of the sample from the sample-vial. The sample manager further includes a sample automation system that includes a second needle drive including a second sample needle configured to add a volume of reagent to the sample-vial.

A liquid chromatography system includes a solvent delivery system, a sample manager including a sample delivery system in fluidic communication with the solvent delivery system, the sample delivery system configured to inject a sample from a sample-vial into a chromatographic flow stream, a liquid chromatography column located downstream from the sample delivery system, and a detector located downstream from the liquid chromatography column. The sample delivery system further includes a first needle drive including a first sample needle configured to extract the sample from the sample-vial and deliver the sample to the liquid chromatography column, and a first syringe in communication with the first sample needle configured to meter extraction of the sample from the sample-vial. The sample manager further includes a sample automation system that includes a second needle drive including a second sample needle configured to add a volume of reagent to the sample-vial.

A method for collecting a sample for sample analysis includes drawing a first portion of the sample into a sample storage portion of a chromatography system while the chromatography system is in a first configuration. The method further comprising switching the chromatography system to a second configuration; sealing an end of a sample pick-up needle and draining a portion of a liquid from the second tubing; switching the chromatography system to a third configuration; drawing a second portion of the sample into the sample storage portion of the chromatography system; switching the chromatography system to an injection configuration; and fluidly connecting the sample storage portion to the chromatography column and supplying the first portion of the sample and the second portion of the sample from the sample storage portion to the chromatography column.

A liquid chromatograph includes at least one mobile phase supply flow path (6; 42), at least one cleaning solution supply flow path (8; 44) joining the mobile phase supply flow path (6; 42), an analysis flow path (4) provided with a separation column (14), a sampling flow path (2) provided, at a tip end thereof, with a sampling needle (12), a switcher (10; 26) including a high-pressure valve (10) having an injection port (16) and configured to switch a flow path configuration of the liquid chromatograph between a loading state in which the analysis mobile phase supply flow path (6) and the analysis flow path (4) are connected in a fluid manner without the sampling flow path (2) and an injecting state in which the sampling flow path (2) is interposed between the analysis mobile phase supply flow path (6) and the analysis flow path (4) when a tip end of the needle (12) is inserted into the injection port (16), and a controller (50) configured to execute, when a predetermined condition is satisfied, the cleaning operation of supplying the mobile phase and the cleaning solution to the analysis flow path (4) and/or the sampling flow path (2) to temporally change the composition of liquid flowing in the analysis flow path (4) and/or the sampling flow path (2).

A liquid chromatograph includes at least one mobile phase supply flow path (6; 42), at least one cleaning solution supply flow path (8; 44) joining the mobile phase supply flow path (6; 42), an analysis flow path (4) provided with a separation column (14), a sampling flow path (2) provided, at a tip end thereof, with a sampling needle (12), a switcher (10; 26) including a high-pressure valve (10) having an injection port (16) and configured to switch a flow path configuration of the liquid chromatograph between a loading state in which the analysis mobile phase supply flow path (6) and the analysis flow path (4) are connected in a fluid manner without the sampling flow path (2) and an injecting state in which the sampling flow path (2) is interposed between the analysis mobile phase supply flow path (6) and the analysis flow path (4) when a tip end of the needle (12) is inserted into the injection port (16), and a controller (50) configured to execute, when a predetermined condition is satisfied, the cleaning operation of supplying the mobile phase and the cleaning solution to the analysis flow path (4) and/or the sampling flow path (2) to temporally change the composition of liquid flowing in the analysis flow path (4) and/or the sampling flow path (2).

A preparative chromatograph includes a separation column, and a detector provided downstream of the separation column. Furthermore, the preparative chromatograph includes a fractionator including a gas-liquid separator configured to separate a fluid containing components of a sample into a gas and a liquid, the fractionator being provided downstream of the detector. The preparative chromatograph is configured to supply carbon dioxide to a flow path between the separation column and the fractionator.

A preparative chromatograph includes a separation column, and a detector provided downstream of the separation column. Furthermore, the preparative chromatograph includes a fractionator including a gas-liquid separator configured to separate a fluid containing components of a sample into a gas and a liquid, the fractionator being provided downstream of the detector. The preparative chromatograph is configured to supply carbon dioxide to a flow path between the separation column and the fractionator.

A chromatography system includes an electrolytic eluent generator; a first valve configured to switch between an operating position which directs an output of the electrolytic eluent generator to a continuously generated trap column and a waste position which directs the output of the electrolytic eluent generator to waste; the continuously regenerated trap column; a degasser; a sample injector including a sample injector valve assembly, the sample injector valve assembly configured to switch between an operation mode which directs an output of the degasser to a separation column, a load mode which loads a sample onto the separation column, and a regenerant mode which directs the output of the degasser to a regenerant line; the separation column; a suppressor; and a detector.

A chromatography system includes an electrolytic eluent generator; a first valve configured to switch between an operating position which directs an output of the electrolytic eluent generator to a continuously generated trap column and a waste position which directs the output of the electrolytic eluent generator to waste; the continuously regenerated trap column; a degasser; a sample injector including a sample injector valve assembly, the sample injector valve assembly configured to switch between an operation mode which directs an output of the degasser to a separation column, a load mode which loads a sample onto the separation column, and a regenerant mode which directs the output of the degasser to a regenerant line; the separation column; a suppressor; and a detector.