A silica-based stationary phase for chromatography columns and the methods of preparing such. More particularly, but not by way of limitation, a silica-based stationary phase that is substantially free of polyethers (e.g., polymer glycols). Also, a chromatography column comprising a silica-based stationary phase substantially free of polyethers (e.g., polymer glycols) within its channels as either a thin-film coating and/or a monolith and/or a monolithic coating. More particularly, a micro-electro-mechanical system (MEMS) chromatograph comprising a silica-based monolith substantially free of polyethers (e.g., polymer glycols) as the stationary phase within the micro-channels of the column.

A silica-based stationary phase for chromatography columns and the methods of preparing such. More particularly, but not by way of limitation, a silica-based stationary phase that is substantially free of polyethers (e.g., polymer glycols). Also, a chromatography column comprising a silica-based stationary phase substantially free of polyethers (e.g., polymer glycols) within its channels as either a thin-film coating and/or a monolith and/or a monolithic coating. More particularly, a micro-electro-mechanical system (MEMS) chromatograph comprising a silica-based monolith substantially free of polyethers (e.g., polymer glycols) as the stationary phase within the micro-channels of the column.

The present invention provides a column filler for liquid chromatography that has a great adsorption capacity, adjustable adsorption selectivity, and high shape retainability and therefore is usable for measurement of various substances and capable of achieving excellent separation performance and a high filling rate in a column when used as a column filler for liquid chromatography. Provided is a column filler for liquid chromatography including carbon-coated porous particles, the carbon-coated porous particles including porous particles each having a coating layer containing an amorphous carbon on a surface.

A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

Disclosed is an equipment including a container filled with chromatography beads for capturing a product present in a process liquid or to prepare them for subsequent use. The equipment also includes a supporting frame standing on the ground and providing support for the container such that the container is spaced above the ground. Outside the container there is provided a lifting system fastened to and supported by the supporting frame and fastened to a part of the container such that the part of the container can be raised by the lifting system relative to the supporting frame and another part of the container. At least part of the lifting system extends aside the container from the bottom to the top of the container.

Disclosed is an equipment including a container filled with chromatography beads for capturing a product present in a process liquid or to prepare them for subsequent use. The equipment also includes a supporting frame standing on the ground and providing support for the container such that the container is spaced above the ground. Outside the container there is provided a lifting system fastened to and supported by the supporting frame and fastened to a part of the container such that the part of the container can be raised by the lifting system relative to the supporting frame and another part of the container. At least part of the lifting system extends aside the container from the bottom to the top of the container.

An ion exchange chromatography column contains an ion exchange stationary phase that includes a charged substrate, a plurality of first particles, and a plurality of second particles. The plurality of first particles each include first ion exchange groups and the first particles are ionically bound to the charged substrate. The plurality of second particles each include second ion exchange groups and the second particles are ionically bound to the charged substrate. The first particles having a first ion exchange group density, and the second particles having a second ion exchange group density. The first ion exchange group density is greater than the second ion exchange group density. The ion exchange chromatography column has a number of zones connected in series where each zone can have a varying level of first ion exchange groups and second ion exchange group from the inlet zone to the outlet zone.

Blood containing cells is brought into contact with a porous surface of a porous material before classification of the cells in the blood by flowing the blood through a microchannel. In an example, the porous material is added to the blood containing the cells and mixed together, thereby bringing the blood containing the cells into contact with the porous surface. In an example, the porous material has particles with the porous surface including polysaccharides. The porous material is added to the blood containing the cells while being suspended in a liquid. In an example, the particles have a predetermined particle size distribution. A median particle size d50V in the volume-based cumulative distribution is 25 to 280 μm.