The present disclosure provides chromatography columns and methods of chromatographic separation using the same. In particular, the disclosure provides modified columns where the cross-sectional area of the column increases in an area near the end of the column in a flared manner. The modified columns can be used in Expanded Bed Absorption (EBA) systems.

The present disclosure provides chromatography columns and methods of chromatographic separation using the same. In particular, the disclosure provides modified columns where the cross-sectional area of the column increases in an area near the end of the column in a flared manner. The modified columns can be used in Expanded Bed Absorption (EBA) systems.