An apparatus for chemical separations includes a first substantially rigid microfluidic substrate defining a first fluidic port; a second substantially rigid microfluidic substrate defining a second fluidic port; and a coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates. The coupler includes a material that is deformable relative to a material of the first substrate and a material of the second substrate. The substrates are clamped together to compress the coupler between the substrates and form a fluid-tight seal.

The object of the invention relates to an extraction cell (100) used in a centrifugal partition chromatograph, which has a cell wall (120) determining a closed extraction chamber (150), as well as an inlet (115) and an outlet (140) ensuring the fluid connection between the extraction chamber (150) and the space outside of the extraction cell (100) formed on essentially opposite parts of the cell wall (120).

The extraction cell (100) according to the invention is constructed asymmetrically from the point of view of the reversibility of the direction of flow used when the centrifugal partition chromatograph is in operation.

A method of testing for discerning substitution of carbohydrate ester includes the step of providing a predetermined amount of a solution. Further, the method also includes adding ammonium hydroxide and sodium borohydride to the solution. The method also includes the step of transferring the solution to an ammonium ion exchange cartridge and collecting the eluate from the cartridge. Also included in the method is the step of analyzing the sample in a mass spectrometer to produce a mass spectrum. Further, the method includes calculating the amu of phosphate and sulfate substitution of the ion and comparing it to the actual amu of the found ion.

A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

In this invention, chromatography is integrated on a centrifugal platform to enable low-cost automated purification. Differing from the traditional chromatography method, purification and separation of a centrifugal compound collecting platform disclosed in the present invention mainly uses a centrifugal force to drive the fluid to flow outward in the radial direction when the motor rotates. The compounds to be separated react with the column packing during the flow, and the compounds with different polarities in the sample are gradually separated. The flow of the fluid can be governed by the motor and the geometry of the fluidic design such that compounds with different characteristics can be separated and collected in different collecting chambers.

Provided herein are apparatus for independently manipulating the temperature of a plurality of reaction vessels, e.g., for automated processing of nucleic acids present in the vessels. Printed circuit boards (PCBs) comprising a mount area arranged to have mounted thereon a through-hole thermoelectric device (TED) to facilitate independent temperature control of reaction vessels are also provided, as well as methods relating to the same.

An apparatus comprising a magnetic assembly and methods for operating the apparatus are provided. The magnetic assembly may be used to manipulate molecules in a liquid preparation, for example to isolate or separate the molecules from the liquid. The magnetic assembly may be used to wash and/or isolate nucleic acid molecules of interest from a liquid preparation.

Implemented is an analysis apparatus column oven equipped with a plurality of columns, which is capable of efficiently performing heat conduction on column basis, recognizing a column cartridge that holds a column, and being easily exchanged on column basis. The column change mechanism 105 includes the ferrule 206, the movable ferrule connector 207, the pipe 208, the fastener pull 209, the fastener fitting 210, the column cartridge presser 211, the slide guide 212, the RFID reader 214, the fixed wall 215, the column changer heat insulating member 216, the fixed bottom plate 217, the receiver 218, and the fixed fitting 219. A plurality of column cartridges 104 can be installed in the heat block 101, and are in contact with the column heat block 103 to control the temperature in the column cartridges 104. The column cartridge 104 is fixed between left and right column change mechanisms 105, and when the fixed column cartridge 104 is opened, the column change mechanism 105 on the left side is moved leftward and opened.

There is provided an integrated system for liquid separation, such as LC, CE, affinity chromatography, and ion exchange chromatography, comprising a column and end-fittings embedded in a plastic material, such as a thermoplastic polymer. The system may further comprise an electrospray emitter directly connected with the outlet of the column, wherein a substantial part of the emitter is covered with the polymer material. There is also provided a method by which a separation column along with the accompanying end fittings for connection with adjacent liquid conduits is embedded in a polymer matrix. This configuration e.g. ensures that the factory-made, correct attachment of the fittings to the column is preserved (since the matrix prevents further user intervention, accidental or otherwise). Accordingly, the responsibility for the correct attachment of the fittings is shifted from the end user to the manufacturer.

An ion exchanger includes a case and a cartridge. The case has an opening open upward. The cartridge is detachably attached to the case through the opening and includes a circumferential wall, a top wall, a lower opening, and a porous body. The cartridge accommodates an ion exchange resin. The porous body closes the lower opening of the cartridge and allows coolant to pass through while not allowing the ion exchange resin to pass through. A discharge hole that allows air inside the cartridge to be discharged out of the cartridge is formed in at least one of the top wall of the cartridge or a portion of the circumferential wall of the cartridge that is opposed to the inner circumferential surface of the case body.