A drive module with a hydraulic circuit that includes a reversible motor, a reversible pump driven by the reversible motor, a hydraulic reservoir, a first conduit coupling a first inlet/outlet of the reversible pump to the hydraulic reservoir, a second conduit coupling a second inlet/outlet of the reversible pump to the hydraulic reservoir, first and second hydraulic cylinders, and first and second one-way valves. The first hydraulic cylinder has a third inlet/outlet that is coupled to the first conduit between the reversible pump and the hydraulic reservoir. The second hydraulic cylinder has a fourth inlet/outlet that is coupled to the second conduit between the reversible pump and the hydraulic reservoir. The first one-way valve is disposed in the first hydraulic conduit between the first hydraulic cylinder and the reservoir. The second one-way valve is disposed in the second hydraulic conduit between the second hydraulic cylinder and the reservoir.

A fraction collector control device includes a peak designation part that displays a chromatogram for setting and requires a user to select at least one peak in the chromatogram for setting, a parameter extraction part that extracts, as a parameter, a signal level and/or a slope at a start point and an end point of each peak designated by a user, and a threshold candidate generation part that generates, based on the parameter extracted by the parameter extraction part, a threshold candidate usable as a common threshold for detecting start points of all peaks designated by a user and a common threshold for detecting end points of all peaks designated by a user.

A variety of methods/systems/apparatus/compositions are disclosed, including, in one embodiment, a method including introducing a polymeric material, an unsaturated compound, and an olefin cross metathesis catalyst into a reactor, wherein the polymeric material comprises a carbon black filler; reacting at least a portion of the polymeric material with the unsaturated compound in the presence of the olefin cross metathesis catalyst to produce at least metathesis oil and metathesis carbon black, wherein the metathesis oil comprises an olefin cross metathesis product of the polymeric material and the unsaturated compound; and separating at least a portion of the metathesis carbon black.

A method for processing chromatographic peaks in chromatographic systems comprising comparing a first peak with a second peak; and determining whether the first peak and second peak should be grouped together.

Systems and methods are disclosed for identifying actual XIC peaks of compounds of interest from samples so that more accurate expected retention times and more accurate expected retention time windows can be calculated. In one system, an actual XIC peak is identified using standard samples. The ratio of the quantity of the compound of interest in any two different samples is known, so this ratios is compared to the intensities of the XIC peak calculated in the two samples to identify an actual XIC peak. In another system, an actual XIC peak is identified using information about other compounds of interest in a plurality of samples. It is known that the XIC peaks of compounds of interest in the same samples have a similar distribution of retention times across those samples, so the distributions of retention times of XIC peaks are compared to identify actual XIC peaks.

The invention discloses a method for correcting a baseline in a chromatogram obtained using a buffer with at least one UV-absorbing component which is a weak acid or a weak base. The method comprises the steps of: a) providing a chromatography system with a chromatography column and a UV detector downstream of the chromatography column; b) providing UV extinction coefficients at a wavelength for the acid form and the base form of the UV-absorbing component(s) and a dissociation constant for the component(s), c) obtaining a chromatogram with the UV detector at wavelength using the buffer under a set of conditions where pH and/or conductivity is varied between these conditions, d) for a plurality of said conditions calculating the concentrations of the acid and base forms using the dissociation constant(s) and values for pH and conductivity values, and e) calculating UV absorption values of the acid and base forms from the concentrations and UV extinction coefficients under the conditions in question and subtracting the UV absorption values from the chromatogram.

The invention relates to a chromatography system and method for assessing amount and/or purity of a multimeric protein in a sample, wherein the chromatography system comprises two different affinity chromatography matrices connected via a switch valve.

A method, for use in a mass spectrometer or computer software, and computer readable medium, for acquiring mass spectral data; comprising acquiring mass spectral data for a sample; selecting a relevant retention time window for presence of possible compounds of interest; using positively identified analytes from a sample run to convert retention time into retention index; determining a retention index range for said relevant retention time window; using the acquired spectral data in said relevant retention time window to perform a spectral library search to identify possible compounds; selecting a subset of possible compounds based on at least one of their retention index values and spectral library search scores; performing a regression analysis, between the spectral data within the retention time window and the library spectrum of at least one of the subset of possible compounds; and reporting the regression coefficients as representative of the concentrations or chromatograms of said possible compounds.

The disclosed signal quality assessment is particularly useful for mass spectrometry. In an embodiment of the disclosed system, a processor utilizes a wavelet-based feature extraction for the signal quality assessment of a chromatogram signal. The signal quality assessment may be used to control operational parameters, such as a flowrate of a pump that moves liquid droplets toward an ionization device of a mass spectrometer.

The instant disclosure provides nucleic acid amplification systems and multi-reaction analysis systems useful in the efficient processing of samples, including clinical samples. Integrated systems that include nucleic acid amplification devices functionally combined with multi-reaction analysis systems are also included. Also provided are methods for monitoring multiple concurrent nucleic acid amplification reactions that include the use of devices and systems described herein.