This tool utilizes orthogonality concepts used for analytical chromatography and apply them to chromatography for downstream processing applications utilizing a small set of product-agnostic, optimally orthogonal resins with which most separations (capture or polishing) can be accomplished. Libraries of components for separation mediums are provided. The library of components is administered to the separation mediums and combination of the separation mediums at varying pHs, and the separability and orthogonality of each is quantified. The separability is a measure of a probability that the separation mediums will separate a pair of components, whereas the orthogonality is a measure of the enhancement in separability upon addition of another separation medium. By identifying those combinations of separation mediums that not only provide advantageous separability, but also high orthogonality, sets of separation mediums can be more easily provided or wholly designed for use in processing applications.

At least one of method information representing an analysis execution method to be used for analysis of a sample and device information for specifying the configuration of an analysis device is acquired as analysis information by an analysis information acquirer. Syringe information for specifying the configuration of a syringe is acquired by a syringe information acquirer. Based on the analysis information and the syringe information, whether the syringe is suitable for analysis of the sample is judged by a judge. The result of judgement by the judge is presented by a presenter.

A method of preparing a liquid mixture for use in a liquid chromatography system is provided. The mixture comprises one or more acids, one or more bases, one or more solvents and water, and the method comprises the steps of: calculating pH and/or solvent concentration at a particular time t from a user-determined gradient function; and, based on the values obtained, calculating percent acid, percent base, percent solvent and percent water in the liquid mixture at time t. A liquid chromatography system incorporating such method is also provided.

Method for determining the influence of least a first and a second experimental parameter on a liquid chromatography protocol for purifying one or more target molecules from a sample, comprising the steps: —performing chromatography purifications of the sample at a plurality of different experimental conditions where at least the first and the second experimental parameter each are varied over a predetermined range, each purification being registered as a chromatogram by monitoring an output parameter indicative of the purification result during the purification; and —displaying in a graphical user interface at least a subset of the registered chromatograms as chromatogram-miniatures in an evaluation diagram wherein the position of each displayed chromatogram-miniature is determined by the experimental parameters for the corresponding purification, thereby allowing a user to visually determine trends and the influence of the experimental parameters on the liquid chromatography protocol.

An automated method of monitoring a state of an analyzer is provided including a mass spectrometer (MS) with an electrospray ionization (ESI) source coupled to a liquid chromatography (LC) stream, including monitoring an electrospray ionization current of the ESI source and identifying a condition of multiple conditions of the analyzer based on the monitored ionization current of the ESI source, one of the conditions being a presence of a dead volume in a liquid chromatography stream of the analyzer downstream of an LC column of the LC stream.

The present invention relates to methods for controlling chromatographic processes in real-time via mass measurement utilizing a variable pathlength spectrophotometer.

The present invention relates to methods for controlling chromatographic processes in real-time via mass measurement utilizing a variable pathlength spectrophotometer.

The present invention relates to a chromatography method, a method of determining the concentration of at least one compound in a chromatography method, a method of obtaining an adsorption isotherm, a method of obtaining at least one stationary phase and a method of evaluating the accuracy of a predetermined adsorption isotherm.

A sample measurement device includes a measurement unit (1) configured to measure a sample, and a controller (2) configured to analyze a measurement result of the measurement unit. The controller (2) is configured to estimate and acquire a measurement result under another measurement condition using a model formula based on measurement results under a plurality of measurement conditions with different measurement parameter conditions, and estimate a distribution of a measurement quality indicator with respect to a measurement parameter based on the estimated measurement result.

The present invention relates to a method for monitoring, wherein the sample injector comprises a sample storage portion and a volume displacement device. The method comprises obtaining a pressure characteristic indicative for a pressure of the sample storage portion. In addition, the method comprises obtaining a displacement characteristic indicative for a displacement volume of the volume displacement device, when the volume displacement device is fluidically connected to the sample storage portion. Based on the obtained pressure characteristic and the obtained measured displacement characteristic the method comprises determining at least one result. In addition, the present invention relates to a sample injector, sample injector system and a system configured to carry out the method.