A derivatization apparatus and method for coating a sample carrier with a reagent solution. The derivatization apparatus consists of a closed system in which a small quantity of reagent solution is sprayed into a closed container via a spray nozzle. A sample carrier is located in the closed container and is coated as homogeneously as possible with the reagent solution.

Provided is a thin layer chromatography plate that makes it possible to separate a plurality of components from each other more simply and more quickly. Thin layer chromatography plate includes substrate and separation layer that is disposed on substrate for separating multiple components included in a sample from each other. Separation layer has first layer that has a band shape and extends in first development direction and second layer that extends in second development direction orthogonal to first development direction. Second layer is in contact with first layer. First layer includes a hydrophilic porous body. Second layer includes a hydrophobic porous body.

A method for simulataneously separating and detecting aldehydes or ketones from a plurality of samples containing the samein a simple and rapid manner by using a rotary microdevice capable of integrating derivatization and TLC separation of aldehydes and ketones, and the method providing reliable TLC separation, control of moving speed of an eluent on a TLC plate, and improved TLC resolution.

A method for performing thin-layer chromatography applies a liquid sample to a separating layer. A mobile phase is subsequently applied to the separating layer and brought into contact with the liquid sample. After a developmental phase, a thin-layer chromatogram is produced. The liquid sample is taken up from a sample storage container with the aid of an autopipette using a new pipette tip for each sample application operation and applied to the separating layer. A device for performing the method includes a support plate for the accommodation of the separating layer, a first storage container for the accommodation of the sample, and a second storage container for the accommodation of the mobile phase.

The present disclosure provides a colloidal gold immunochromatographic joint inspection card for diquat and paraquat in the field of on-site rapid detection, as well as a preparation method and application. The joint inspection card includes a PVC base plate, where the PVC base plate is sequentially provided with a sample pad, a gold-labelled pad, a nitrocellulose membrane and a water-absorbing pad along the chromatographic direction; the gold-labelled pad is adsorbed with an antibody solution labeled with paraquat colloidal gold and an antibody solution labeled with diquat colloidal gold; the nitrocellulose membrane is provided with a T1 detection line, a T2 detection line and a quality control line along the chromatographic direction; and an area of the T1 detection line is coated with paraquat complete antigen, an area of the T2 detection line is coated with diquat complete antigen, and the quality control line is coated with goat anti-mouse IgG.

To develop the chromatogram, a prepared plate is placed in a chromatographic chamber where a tip, connected to multiple supply lines for different eluent components, enters from below. Each line includes a reservoir, pump, and tubing system. The tip is moved along a set path by a three-dimensional machine while the eluents are pumped with varying flow rates, controlled by a computer to produce a changing eluent composition over time. A digital camera tracks the position of the eluent front, and this data is used by the computer to adjust pump operations in real-time. Once the eluent front reaches its final position, pumping stops. The plate is then removed and dried, resulting in a developed chromatogram.