A fluid sensor system that is configured to perform in-line monitoring is disclosed. The fluid sensor system can include a sensor module that includes a sensing channel configured to receive a sample fluid, two or more calibration compartments, and a sensing element configured to interact with the sample fluid in the sensing channel. The fluid sensor system can include a reader that is electrically and mechanically coupled to the sensor module. The reader includes a controller that is configured to control operation of the fluid sensor system.

Apparatuses and methods related to a point-of-care portable assay device are described herein. In an embodiment, a device for analyzing a fluid sample includes a fluid actuation source configured to engage a port on a test card. The fluid actuation source is configured to apply pressure to the port to cause the fluid sample to move along a microchannel of the test card to enable one or more assays to be performed. The device also includes a controller configured to receive an indication as to which one or more assays are to be performed, cause the fluid actuation source to move the fluid sample into one or more regions on the test card, cause a plurality of electrical contacts to provide power to the test card for performing the one or more assays, and determine at least one test result based on the performed assays.

The present invention involves a fill needle system for aseptically dispensing a pharmaceutical fluid in an aseptic chamber comprises a fill needle tubing in fluid communication with a pharmaceutical fluid source via flexible tubing and extending through a fill needle hub; a fill needle dispensing tip disposed at a dispensing end of the fill needle tubing; a fill needle sheath shaped and arranged to removably mate with and seal aseptically to the fill needle hub to form an aseptically sealed volume enclosing the dispensing tip; and a fluid pressure pulse induction system disposed and configured to compress the flexible tubing in order to dislodge droplets of pharmaceutical fluid retained on the dispensing tip after halting dispensing of the pharmaceutical fluid. An associated method of dispensing pharmaceutical fluid comprises operating the fluid pressure pulse induction system to dislodge the droplets. The system may comprise a controller for automatically controlling the dispensing and droplet dislodging.

According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

An apparatus for classifying a picture of a reaction of reactants in a predetermined container; the apparatus comprising a first neural network arranged for receiving an input picture of a reaction of reactants in a predetermined container and for providing, for each reaction category of a first plurality of reaction categories, a probability that the input picture shows a reaction that belongs to said reaction category; a second neural network arranged for, if the first neural response provides a highest probability that the input picture shows a reaction that belongs to a predetermined reaction category, receiving a predetermined portion of the input picture and providing, for each reaction category of a second plurality of reaction categories, a probability that said predetermined portion of the input picture shows a reaction that belongs to said reaction category.

In some aspects, automated rapid antimicrobial susceptibility testing systems for performing a multi-assay testing sequence can include an automated incubation assembly having a nest assembly adapted to house at least one test panel having a plurality of wells for receiving a sample comprising microorganisms originating from a clinical sample, the incubation assembly facilitating incubation of one or more test panels in order to undergo the multi-assay testing sequence; a robotic handling assembly configured to accept one or more incoming test panels and move them to and from the incubation assembly for incubation between each assay of the multi-assay testing sequence; an automated liquid handling assembly configured to exchange one or more fluids in the plurality of wells of the test panels; and an optical assembly for interrogation and readout of each assay of the multi-assay testing sequence being performed in the plurality of wells.

Apparatuses and methods related to a point-of-care portable assay device are described herein. In an embodiment, a device for monitoring a reaction in a fluid sample includes a vacuum source configured to pull the fluid sample through a microchannel, a current source configured to cause the reaction while the fluid sample is located within the microchannel, a light source configured to illuminate the reaction while the current source causes the reaction, a camera imaging device configured to record an image of the reaction while the light source illuminates the reaction, and a controller configured to analyze the image of the reaction and output a resulting analysis of the reaction.

Flow cells and corresponding methods are provided. The flow cells may include a support frame with top and back sides, and at least one cavity extending from the top side. The flow cells may include at least one light detection device with an active area disposed within the at least one cavity. The flow cells may include a support material disposed within the at least one cavity between the support frame and the periphery of the at least one light detection device coupling them together. The flow cells may include a lid extending over the at least one light detection device and coupled to the support frame about the periphery of the at least one light detection device. The lid and at least a top surface of the at least one light detection device form a flow channel therebetween.

The present invention involves a fill needle system for aseptically dispensing a pharmaceutical fluid in an aseptic chamber comprises a fill needle tubing in fluid communication with a pharmaceutical fluid source via flexible tubing and extending through a fill needle hub; a fill needle dispensing tip disposed at a dispensing end of the fill needle tubing; a fill needle sheath shaped and arranged to removably mate with and seal aseptically to the fill needle hub to form an aseptically sealed volume enclosing the dispensing tip; and a fluid pressure pulse induction system disposed and configured to compress the flexible tubing in order to dislodge droplets of pharmaceutical fluid retained on the dispensing tip after halting dispensing of the pharmaceutical fluid. An associated method of dispensing pharmaceutical fluid comprises operating the fluid pressure pulse induction system to dislodge the droplets. The system may comprise a controller for automatically controlling the dispensing and droplet dislodging.