A solution mixer comprising: a main flow path in which a solution circulates; at least one solution introduction flow path connected to the main flow path; and at least one solution discharge flow path connected to the main flow path, wherein the solution discharge flow path has at least one solution discharge flow path valve, and wherein the main flow path has at least one main flow path valve.

An analysis device that includes a placement section, a pressing member, and a measurement member, has an analysis kit including a chip provided with a capillary through which a sample flows and a cartridge superimposed on the chip, and in which a liquid reservoir placed therein to enable a component present in sample can be measured in a state in which the chip and the cartridge have been fitted together. The analysis kit is placed on the placement section. The pressing member presses the analysis kit in a direction in which the cartridge is superimposed on the chip to sandwich the analysis kit between the pressing member and the placement section, and to fit the chip and the cartridge together. The measurement member that measures the component present in the sample in the analysis kit in which the chip and the cartridge have been fitted together.

A cartridge can comprise a first elastomeric membrane and a second elastomeric membrane, and portions of the elastomeric membranes which are sealed to each other can circumscribe unsealed portions of the membranes. In a resting state, the unsealed portion of the first elastomeric membrane abuts or is proximate to the unsealed portion of the second elastomeric membrane. One or more reagents can be injected between the unsealed portions of the first and second elastomeric membranes to push the unsealed portions apart from each other in this region of the membranes. The unsealed portions can be sequentially pushed apart in downstream regions to form a channel between the elastomeric membranes. Positively displaced fluid pushes the unsealed membrane portions apart to a volume that conforms to the volume of the fluid to minimize or prevent dead volume in the channel and thus minimize or prevent air bubbles in the fluid.

The present invention provides self-contained systems, apparatus and methods for determining a chemical state, the system includes a stationary cartridge for performing the assay therein, the cartridge adapted to house at least one reagent adapted to react with a sample; and at least one reporter functionality adapted to report a reaction of the at least one reagent with the sample to report a result of the assay, a mechanical controller including a first urging means adapted to apply a force externally onto the cartridge to release the at least one reagent; and at least one second urging means adapted to apply a removable force to induce fluidic movement in a first direction in the cartridge and upon removal of the force causing fluidic movement in an opposite direction to the first direction, an optical reader adapted to detect the reaction and a processor adapted to receive data from the optical reader and to process the data to determine said chemical state.

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.

A system for fluorescence activated cell sorting includes at least two excitation lasers having different orientations relative to an objective such that light from the at least two lasers passes through the objective and intersects a fluidic channel at different positions within an interrogation region. The fluidic channel directs a flow of a plurality of fluorescently labeled particles through the interrogation region. The system further includes at least one detector and at least one optical element that directs light emitted from the plurality of fluorescently labeled particles and transmitted through the objective to the at least one detector. The system may further include optics for generating and detecting side and forward scattered light. Methods for operating example systems to collect fluorescent, side scattered and forward scattered light from a plurality of particles are also described herein.

A rotor assembly includes a rotor plate to rotate around a first axis, a bucket attached to the rotor plate and to rotate around a second axis, and a stop plate to rotate around the first axis between an open position and a closed position. When in the closed position, the stop plate engages the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly. The rotor assembly further includes a housing for a sensor array component, the housing disposed in the bucket and including a solution inlet, a solution outlet, a transfer basin, a solution retainer disposed between the solution outlet and the transfer basin, and a collection reservoir in fluid communication with the transfer basin. The solution inlet and the solution outlet to engage ports of a flow cell of a sensor array.

The present invention discloses a kind of centrifugal detection channel, including: a first chamber, at least one inlet channel, at least one buffer valve, at least one outlet channel, and a second chamber. Specifically, at least one inlet channel is connected to the first chamber. At least one buffer valve is connected to the inlet channel, including: a valve body and a transient chamber. At least one outlet channel is connected to the buffer valve. At least one second chamber is connected to the outlet channel. In addition, the detection devices and the detection methods comprising the centrifugal detection channel have also been proposed.

Provided herein is technology relating to testing biological samples and particularly, but not exclusively, to devices, systems, and kits for performing multiple, simultaneous real-time assays on a sample in a single-use disposable format. For example, the technology relates to an apparatus that finds use, for example, for point-of-care diagnostics, including use at accident sites, emergency rooms, in surgery, in intensive care units, as well as for non-medical applications.