An analyzer system includes a cartridge configured to receive a sample. The cartridge has a plurality of chambers for isolating a target analyte of the sample and collecting a quantity of a first label that is proportional to a quantity of the target analyte in the sample. The system includes an analyzer with a first electromagnetic radiation source a first detector and a controller. The first electromagnetic radiation source is configured to provide electromagnetic radiation to form an interrogation space within a detection chamber of the cartridge. The first detector is configured to detect electromagnetic radiation emitted in the interrogation space by the first label if the first label is present in the interrogation space. The controller is configured to identify the presence of the target analyte in the sample based on electromagnetic radiation detected by the first detector.

Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

A microfluidic device includes a plurality of reaction wells; and a plurality of solid supports, and each of the solid supports has a reagent attached thereto. The reagent is attached to the solid support via a labile reagent/support bond such that the reagent is configured to be cleaved from the support via a cleaving operation.

The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

An apparatus comprising a magnetic assembly and methods for operating the apparatus are provided. The magnetic assembly may be used to manipulate molecules in a liquid preparation, for example to isolate or separate the molecules from the liquid. The magnetic assembly may be used to wash and/or isolate nucleic acid molecules of interest from a liquid preparation.

A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

Evaluating an effect of one or more perturbations on cells of a first cell type is described. One method includes obtaining a screen definition for a screen, where the screen includes a cell-based assay that is run on a temporarily contiguous basis using a plurality of multi-well plates. The method includes obtaining control vectors including measurements of corresponding features of cells in control wells. The method includes obtaining test vectors including measurements of corresponding features of cells in test wells. The method includes forming a variability model based on a variance across the of control vectors, and embedding test vectors onto the variability model, thereby obtaining a set of variability model values. The method includes using the set of variability model values to resolve an effect of at least one data perturbation in the plurality of data perturbations on the first cell type.

An analysis device includes a guide-in section, a placement section, an illumination member, a pressing member, and a measurement member. The guide-in section is configured to guide a rectangular block shaped analysis kit containing a sample. The analysis kit is placed on the placement section in the guide-in section. The illumination member is inserted into an insertion hole formed in the analysis kit, contacts a bottom of the insertion hole, and illuminates light onto the sample. The pressing member is configured by a separate body to the illumination member and presses the analysis kit such that the analysis kit placed on the placement section is sandwiched between the pressing member and the placement section and retained at a predetermined position. The measurement section measures a component present in the sample using light illuminated from the illumination member onto the sample in the analysis kit placed on the placement section.

A method for preparing and processing a sample is provided. The method includes obtaining a sample including biofluid. The method further includes purifying at least part of the sample via an acoustic separator to separate target cells from the sample. The sample may accordingly be at least partially purified. The method further includes causing a portion of an output collected from the acoustic separator to flow through a filter. At least one reagent, such as a lysis reagent or assay reagent, is caused to flow over the cells.

A rotor assembly includes a rotor plate to rotate around a first axis; a bucket rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate to rotate around the first axis relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate is to engage the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.