Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior.

In an example of an embodiment, a first sample set is prepared that includes a test sample prepared from a first subject sample and a reagent, and at least one control sample prepared from at least one of a positive control and a negative control, and a second sample set is prepared that includes a test sample prepared from a second subject sample and a reagent and does not contain at least one of the control samples contained in the first sample set. The nucleic acid amplification in the first sample set is measured in the first unit, the nucleic acid amplification in the second sample set is measured in the second unit, and the measurement result of each test sample contained in the first sample set and second sample set is analyzed based on the measurement result of the control sample contained in at least the first sample set.

In one embodiment, a method is provided comprising analyte testing on one or more types of samples.

A module installation unit is capable of installing a plurality of modules capable of accommodating a plurality of tubes containing a sample. A temperature adjusting unit heats and cools the sample in the tube of each module to the temperature required for nucleic acid amplification. An optical detection unit is used commonly by the plurality of modules installed in the module installation unit and which is capable of detecting the amplified nucleic acid of a sample subjected to nucleic acid amplification of a tube by regulating the temperature via the temperature adjusting unit for each module installed in the module installation unit. A moving unit moves the optical detecting unit and module installation unit relative to each other so as to detect the amplified nucleic acid of the sample in a tube of each of the plurality of modules installed in the module installation unit via the optical detection unit.

Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

It is necessary to dispose a plurality of units which have different target temperatures on an apparatus in a more integrated state in order to improve analysis performance and processing capacity per unit space of the apparatus. Therefore, it is necessary to mitigate influence of temperature exerted between units. It is possible to reduce the temperature influence exerted between the units and thus efficiently control temperature by properly disposing the units in the apparatus, based on the relationship between a use environment temperature of the apparatus and a target temperature of each unit and temperature accuracy required for the unit.

In one embodiment, a biological sample analyzer has a housing having at least one outer wall that defines a cavity therein, an air intake, and an air exhaust. A plenum is disposed within the cavity and has at least one plenum wall that defines an air duct therein. A receptacle, which can support a consumable holder containing a biological sample, is disposed within the internal cavity. At least a portion of the receptacle is supported within the air duct such that an air gap is defined between the receptacle and the at least one plenum wall. A heater applies heat to the consumable holder so as to heat the consumable holder when the consumable holder is supported by the receptacle. A fan forces air along a path that extends from the air intake, through the air gap, and to the air exhaust so as to cool the heater.

An analytic device comprising a device housing, a dock to receive a camera enabled mobile electronic device, such as a smartphone and other smart devices, and a processing device to communicate with the mobile electronic device and to control a condition of the assay tube, such as temperature. In another example, the analytic device comprises a device housing and a circuit board. A processing device, a heating block defining a recess to support assay tube, and a resistive heater are surface mounted to the circuit board. A light source and a fan are also provided. A dock may be provided to support a mobile electronic device. The mobile electronic device communicates with the processing device to cause the application of reaction conditions to the assay tube, to perform a PCR procedure, for example. Methods are also disclosed.

An instrument for processing a biological sample includes a chassis. Connected to the chassis is a tape path along which a tape with a matrix of wells can be automatically advanced through the instrument, a dispensing assembly for dispensing the biological sample and a reagent into the matrix of wells of the tape to form a biological sample and reagent mixture, a sealing assembly for sealing the biological sample and reagent mixture in the tape, and an amplification and detection assembly for detecting a signal from the biological sample and reagent mixture in the matrix of wells in the tape.

The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).