The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

A matrix droplet extruder includes one or a plurality of reagent containers. Pneumatic connectors are each connectable to a corresponding docking station connector of a docking station that is connectable to a pneumatic generator controllable by a controller to provide pneumatic pressure to the pneumatic docking station connectors. A droplet matrix extrusion surface includes an array of perforations. A liquid management chip has a network of dispensing channels for dispensing reagents from reagent containers through the array of perforations. A pneumatic control network includes pneumatic channels and gates that are controllable by application of the pneumatic pressure to the gates via the pneumatic channels to enable or block dispensing the one or more reagents to repeatedly generate a matrix of droplets when applying the pneumatic pressure to the reagents.

A nucleic acid extraction system, comprising: a nucleic acid extraction plate, and a pipetting device and positive pressure device that are arranged side by side, the pipetting device comprising: an infusion device, an infusion device mounting frame, an infusion device driving unit, and a plurality of infusion units, each of the infusion units comprising: a reagent supply device and a reagent flow controller; the positive pressure device comprises: a plug, an air pipe, a positive pressure provider and a positive pressure driving device. The nucleic acid extraction system is highly efficiency and low-cost when extracting nucleic acid.

A system for fluid transport is provided where a quantity of fluid is held in a reservoir. A droplet generator is employed to generate droplets from the fluid, for example a nozzle-based system or a nozzleless system such as an acoustic ejection system. A generated droplet has a trajectory whereby it arrives at a target. A circuit is used to modify one or more characteristics of the generated droplet in a way which increases the likelihood that the droplet will not splash or bounce when it arrives at the target. The circuit may in different embodiments control the speed of the droplet or the Weber number of the droplet. The circuit may create an electric field in an area of space where the droplet passes. The circuit may charge the droplet by causing it to contact ions.

It is an object to provide a deforming element-included dispensing tip, a deforming element-included dispensing device, and a deforming element-included dispensing processing method not requiring any cylinder and capable of achieving highly precise position control and enhancing integration of higher density and reducing the burden in the quality control in reaction processing of a biological material for extraction of DNA and the like. A tip-shaped container formed with a non-deforming wall having an opening portion at an upper side and an inlet unit at a lower side for allowing fluid to flow in and flow out and a sealing plug attached to the opening portion so as to seal the opening portion are provided, and the sealing plug is configured to be provided with a deforming element.

A system for preparing biological sample contains a body including a proximal side and a distal side, a plurality of mandrels, a plurality of resilient elements, a plurality of fluid dispensers, and one or more samples. The mandrels are moveably positioned within the body, where each resilient element engages a respective one of the mandrels. Each of the fluid dispensers is configured to engage a distal end of a corresponding one of the mandrels. Each sample comprises a solution containing one or more nucleic acid sequences contained within at least one of the fluid dispensers.

There are prepared a first container which stores a liquid containing a cellular aggregate, a second container which receives a cellular aggregate, and a third container which stores a preliminary treatment solution, and a cylinder tip. The cylinder tip is formed with a syringe including a tubular passage having a front end opening which sucks the cellular aggregate, and a plunger which reciprocates in a tubular passage. Before sucking the cellular aggregate from the first container and discharging the same to the third container, the preliminary treatment solution is retained in a space between the tubular passage and the plunger by dipping the front end opening of the cylinder tip into the preliminary treatment solution in the third container and causing the plunger to reciprocate.

In one example in accordance with the present disclosure, a fluidic ejection system is described. The fluidic ejection system includes a frame to retain a number of fluidic ejection devices. The frame has a form factor to match a titration plate. The fluidic ejection system also includes the number of fluidic ejection devices disposed on the frame. Each fluidic ejection device includes a reservoir disposed on a first side of the frame and a fluidic ejection die disposed on an opposite side of the frame. Each fluidic ejection die includes an array of nozzles, with each nozzle including an ejection chamber, an opening, and a fluid actuator disposed within the ejection chamber.

Disclosed herein are fluid handling devices that include integrated card and pipette tip devices, for use with multichannel liquid dispensing devices.

A matrix droplet extruder includes one or a plurality of reagent containers. Pneumatic connectors are each connectable to a corresponding docking station connector of a docking station that is connectable to a pneumatic generator controllable by a controller to provide pneumatic pressure to the pneumatic docking station connectors. A droplet matrix extrusion surface includes an array of perforations. A liquid management chip has a network of dispensing channels for dispensing reagents from reagent containers through the array of perforations. A pneumatic control network includes pneumatic channels and gates that are controllable by application of the pneumatic pressure to the gates via the pneumatic channels to enable or block dispensing the one or more reagents to repeatedly generate a matrix of droplets when applying the pneumatic pressure to the reagents.