A nucleic acid amplification in-situ real-time detection system and method using a micro-fluidic chip through optical fiber sensing. The system includes a white light source, a detection optical path, a microfluidic chip and a spectrum acquisition, processing and display module, which are connected in sequence. The detection optical path is configured to transmit white light from the white light source to the micro-fluidic chip and transmit an optical signal made by the microfluidic chip to the spectrum acquisition, processing and display module. The micro-fluidic chip is configured to carry out biochemical reaction; the spectrum acquisition, processing and display module is configured to acquire the optical signal, analyze the signal and generate a visual biochemical reaction real-time dynamic-change signal curve. This microfluidic chip real-time detection device detects nucleic acid amplification information by using a white light interfered hyperspectral method, so fluorescence-labeled analyte and non-fluorescence-labeled analyte are detected.

The phosphorescence oxygen analyzer has a light source including an LED array that flashes light at 1,000 flashes per second. The light flashes are received in a test chamber containing a carousel having a plurality (preferably ten) of sample vials mounted thereon. The samples a phosphorescent probe (palladium(II) complex, namely, meso-tetra-(4-sulfonatophenyl)tetrabenzoporphyrin; Pd phosphor) mixed with either a control sample of tissue or a sample of tissue and a suspected toxin or a pharmaceutical it is desired to test, the carousel being rotated to irradiate each vial in turn. The probe has an absorption maximum at 625 nm and emission maximum at 800 nm. Phosphorescent emissions are detected by a photomultiplier tube connected to a measurement 2020 board, which is connected to a processor that computes the lifetime and peak of the pulses, which determines the rate of phosphorescent decay due to oxygen metabolized by the tissue mitochondria.

Aspects relate to on-line compensation of instrumental drifts in miniaturized spectrometers due to variations in environmental conditions and due to other sources of instrumental drift. The spectrometer may include a light modulator, a detector, and a processor. The spectrometer may further include a sensor configured to obtain a value of a condition contributing to instrumental drifts in the spectrometer. The processor may be configured to extract a set of correction parameters from a correction matrix associating a plurality of sets of correction parameters with sensor values based on the value and to apply the set of correction parameters to an output of the detector to produce a corrected spectrum of a sample under test. The correction matrix may be generated for the spectrometer or may be based on a global correction matrix fitted to the spectrometer.

The present invention is directed to a gas detector configured to improve response speed of a gas sensor. A gas detector includes a housing, a gas sensor main body installed in the housing, and a partition wall provided in the housing and limiting the surrounding of the gas sensor main body to separate from the other area. The housing or the partition wall includes an opening portion directly connected from the outside to an area inside the partition wall.

A method and system are presented for use in measuring one or more characteristics of patterned structures. The method comprises: performing measurements on a patterned structure by illuminating the structure with exciting light to cause Raman scattering of one or more excited regions of the pattern structure, while applying a controlled change of at least temperature condition of the patterned structure, and detecting the Raman scattering, and generating corresponding measured data indicative of a temperature dependence of the detected Raman scattering; and analyzing the measured data and generating data indicative of spatial profile of one or more properties of the patterned structure.

An optical system for performing an absorption measurement of a medium sample includes a laser source configured to output a laser beam having a wavelength corresponding to an absorption region of interest; a ringdown cavity comprising a chamber configured to receive the medium sample, an input mirror at an input end, an output mirror at an output end, and an optical axis that extends through the centers of the input mirror and the output mirror; a coupling device configured to couple the laser beam through the input mirror into the chamber; and a detector optically coupled with the cavity, and configured to detect an intensity of light of the wavelength corresponding to the absorption region of interest that extends through the output mirror, wherein a cavity geometry of the cavity increases the re-entrant condition of the cavity relative to a conventional cavity comprised of two spherical mirrors.

Optical techniques for determining thermal properties of materials are described. Optical techniques include Raman scattering and thermal properties include relative length change and coefficient of thermal expansion. Correlations of features of bands observed in the Raman spectra of several glasses with thermal properties of the glasses are demonstrated. The technique provides a convenient method for determining thermal expansion properties of materials.

The present invention is directed to a gas detector configured to improve response speed of a gas sensor. A gas detector includes a housing, a gas sensor main body installed in the housing, and a partition wall provided in the housing and limiting the surrounding of the gas sensor main body to separate from the other area. The housing or the partition wall includes an opening portion directly connected from the outside to an area inside the partition wall.

Quantitative colorimetric carbon dioxide detection and measurement systems are disclosed. The systems can include a gas conduit, a colorimetric indicator adapted to exhibit a color change in response to exposure to carbon dioxide gas, a temperature controller operatively coupled to the colorimetric indicator and configured to control the temperature of the colorimetric indicator, an electro-optical sensor assembly including a light source or sources adapted to transmit light to the colorimetric indicator, and a photodiode or photodiodes configured to detect light reflected from the colorimetric indicator and to generate a measurement signal, and a processor in communication with the electro-optical sensor assembly. The processor can be configured to receive the measurement signal generated by the electro-optical sensor assembly and to compute a concentration of carbon dioxide based on the measurement signal. Methods for using the systems are also disclosed including providing a breathing therapy to a patient or user.

A device for monitoring the spatial and temporal dynamics of thrombin that includes a temperature-controlled sealed chamber with a transparent window and a light trap, the chamber being filled with a fluid medium and designed to accommodate a cuvette containing a test sample of blood plasma, and a coagulation activating insert placed into the cuvette, at least one illumination source and at least one first irradiation source and at least one second irradiation source capable of exciting a fluorescence signal of a special marker that forms in the sample during cleavage of a fluorogenic substrate, a camera, a pressure adjustment element capable of maintaining a pressure inside the chamber, the at least one first irradiation source provides irradiation of the sample in a direction perpendicular to the cuvette, and the at least one second irradiation source provides irradiation of the sample at an angle to the cuvette.