An optical detection system includes a sample portion accommodating a sample, a wave source emitting waves to the sample portion, an optical portion provided on a path of an output wave output from the sample portion, and comprising a first spatial light modulator that modulates part of the output wave to a first wave and a second spatial light modulator that modulates part of the output wave to a second wave, a lens portion focusing the first wave and the second wave output from the optical portion, and a detection portion detecting a focused wave that is focused by the lens portion, in which the first spatial light modulator and the second spatial light modulator modulate the output wave such that the first wave and the second wave have destructive interference with respect to the sample under an already known condition.

A fluctuation-based fluorescence microscopy method, comprising influencing a point-spread function of the imaging of a sample emitting fluorescence radiation using an optical device in dependence on a parameter such that a point emitter is imaged into a representation with two image lobes. The relative positions of the lobes depend on the position of the point emitter relative to the focal plane. Synthetic pixels, smaller than detector pixels, are generated; for each synthetic pixel, pairs of pixel groups are defined among pixels of the detector based on the influencing of the point spread function. Each pair is assigned to an individual value of the parameter. In each frame and for each synthetic pixel, a signal correlation is ascertained and allocated as image brightness to the synthetic pixel for the parameter specification. Subframes for each frame are produced from the synthetic pixels, and a high-resolution sample image is produced from the subframes.

Disclosed are methods and apparatus for facilitating defect detection in a multilayer stack. The method includes selection of a set of structure parameters for modeling a particular multilayer stack and a particular defect contained within such particular multilayer stack and a set of operating parameters for an optical inspection system. Based on the set of structure and operating parameters, an electromagnetic simulation is performed of waves scattered from the particular multilayer stack and defect and arriving at a collection pupil of the optical inspection system. Based on the simulated waves at the collection pupil, a design of a phase filter having a plurality of positions for changing a plurality of phases within a plurality of corresponding positions of the collection pupil of the optical inspection tool is determined so as to compensate for an adverse effect of the particular multilayer stack on obtaining a defect signal for the defect within such particular multilayer stack and/or to enhance such defect signal. The design of the phase filter is then provided for fabrication or configuration of a phase filter inserted within the optical inspection system for detection of defects in multilayer stacks with the same structure parameters as the particular multilayer stack. Methods and systems for inspecting a multilayer stack for defects are also disclosed.

Majorana photons are transmitted through a biological tissue sample to image the tissue. The Majorana photons have a circular polarization, a radial polarization or an azimuthal polarization. The transmitted photons are processed to produce a digital image of the biological tissue sample.

Systems and methods for hyperspectral imaging are described. In one implementation, a hyperspectral imaging system includes a sample holder configured to hold a sample, an illumination system, and a detection system. The illumination system includes a light source configured to emit excitation light having one or more wavelengths and a diffractive element. The illumination system is configured to structure the excitation light into a predetermined two-dimensional pattern at a conjugate plane of a focal plane in the sample, spectrally disperse the structured excitation light in a first lateral direction, and illuminate the sample in an excitation pattern with the one or more wavelengths dispersed in the first lateral direction.

An optical detection system includes a sample portion accommodating a sample, a wave source emitting waves to the sample portion, an optical portion provided on a path of an output wave output from the sample portion, and comprising a first spatial light modulator that modulates part of the output wave to a first wave and a second spatial light modulator that modulates part of the output wave to a second wave, a lens portion focusing the first wave and the second wave output from the optical portion, and a detection portion detecting a focused wave that is focused by the lens portion, in which the first spatial light modulator and the second spatial light modulator modulate the output wave such that the first wave and the second wave have destructive interference with respect to the sample under an already known condition.

In a method for measuring radiation, the radiation is temporally and/or spatially separated by a modulator to direct at least N different combinations of radiation incident on each region into at least two and fewer than N distinct directions. The total intensity of radiation in each direction is measured with a detector for each modulator configuration and the detector outputs are analyzed statistically to obtain information relating to the spectral properties of the radiation. In this way substantially all of the energy received at the entrance aperture of a measurement device is encoded into multiple outputs and the multiplexed output is received by a small number of detectors.

An optical detection system includes a sample portion accommodating a sample, a wave source emitting waves to the sample portion, an optical portion provided on a path of an output wave output from the sample portion, and comprising a first spatial light modulator that modulates part of the output wave to a first wave and a second spatial light modulator that modulates part of the output wave to a second wave, a lens portion focusing the first wave and the second wave output from the optical portion, and a detection portion detecting a focused wave that is focused by the lens portion, in which the first spatial light modulator and the second spatial light modulator modulate the output wave such that the first wave and the second wave have destructive interference with respect to the sample under an already known condition.

A photostimulation apparatus includes an objective lens arranged to face a biological object, a light source configured to output light to be radiated toward the biological object via the objective lens, a shape acquisition unit configured to acquire information about a shape with a refractive index difference in the biological object, a hologram generation unit configured to generate aberration correction hologram data for correcting aberrations due to the shape with the refractive index difference on the basis of the information acquired by the shape acquisition unit, and a spatial light modulator on which a hologram based on the aberration correction hologram data is presented and which modulates the light output from the light source.

A liquid crystal photoelectric apparatus includes a first and a second quartz glass substrates, an upper alignment layer disposed between the first and the second quartz glass substrates, a lower alignment layer disposed between the upper alignment layer and the second quartz glass substrate, a liquid crystal material disposed between the upper and the lower alignment layers, a first transparent conductive layer disposed between the upper alignment layer and the first quartz glass substrate and including at least one first main portion and first finger portions extending from the corresponding first main portion and a second transparent conductive layer second transparent conductive layer disposed between the lower alignment layer and the second quartz glass substrate and including a second main portion and second finger portions extending from the second main portion in an extension direction perpendicular to that of the first finger portions. An optical imaging processing system is provided.