Provided is a fluorescence image acquisition apparatus for acquiring fluorescence images and phase images using optical signals that are modulated at the same frequency and that have different time delays. The fluorescence image acquisition apparatus may include a light source configured to generate, at different time delays, a plurality of optical signals that are modulated at the same frequency, an illuminator configured to control paths of the plurality of modulated optical signals so that the plurality of modulated optical signals are illuminated onto a sample including a plurality of fluorescent materials, a photodetector configured to detect a plurality of fluorescence signals that are emitted from the plurality of fluorescent materials, respectively, and a controller configured to acquire a plurality of fluorescence images and a plurality of phase images from the plurality of detected fluorescence signals.

An optical module for Raman spectroscopy includes a laser source mounted on a substrate and configured to emit electromagnetic radiation at a target. The optical module also includes a plurality of sensors mounted on the substrate and configured to detect electromagnetic radiation scattered from the target. The optical module further includes a first filter disposed over one or more of the plurality of sensors. The first filter is substantially transparent to a first wavelength band corresponding to a Raman scattering wavelength of a first molecule of the target and opaque to wavelengths outside the first wavelength band.

An inline concentration meter includes a light source unit emitting mixed light containing at least two wavelengths with a phase difference, a detecting unit including a light incident part for entering the mixed light emitted from the light source unit into a fluid passage of a detector body and at least two light detection parts receiving the mixed light passed through the fluid passage, a computing processor unit conducting frequency analyzes of detection signals of the mixed light output from the respective light detection parts and computing variations of intensities of the detection signals corresponding to absorbances in at least two frequency ranges to compute a concentration of fluid in the fluid passage based on the variations of the intensities of the detection signals, and a recording/displaying unit recording and displaying a value of the fluid concentration computed at the computing processor unit.

A modular testing device includes a base unit and an expansion unit that communicates with the base unit. The expansion unit includes a housing, a receptacle in which a sample holder containing a biological sample and reagent mixture can be placed, and an optical assembly positioned in the housing. The optical assembly is configured to amplify and detect a signal from the biological sample and reagent mixture. Data that is collected in the optical assembly is communicated to the base unit.

A spectrum adjuster produces a pure analyte spectrum and includes: a dynamic opacity optic that receives input light, receives an adjustment signal, produces primary adjusted light, and produces secondary adjusted light from the input light based on the adjustment signal; a light source in optical communication with the dynamic opacity optic; a detector in optical communication with the dynamic opacity optic and that receives transmitted light from the sample and produces a transmitted light signal based on an amount of transmitted light received; and an adjustment controller that receives the transmitted light signal, produces the adjustment signal, and communicates the adjustment signal to the dynamic opacity optic.

A method and analyzer for identifying or verifying or otherwise characterizing a sample comprising: using or having an electromagnetic radiation source for emitting electromagnetic radiation in at least one beam at a sample, the electromagnetic radiation comprising at least two different wavelengths, using or having a sample detector that detects affected electromagnetic radiation resulting from the emitted electromagnetic radiation affected by the sample and provides output representing the detected affected radiation, and using or having a processor for determining sample coefficients from the output, and identifying or verifying or otherwise characterizing the sample using the sample coefficients and training coefficients determined from training samples, wherein the coefficients reduce sensitivity to a sample retainer variation and/or are independent of concentration.

A spectrum adjuster produces a pure analyte spectrum and includes: a dynamic opacity optic that receives input light, receives an adjustment signal, produces primary adjusted light, and produces secondary adjusted light from the input light based on the adjustment signal; a light source in optical communication with the dynamic opacity optic; a detector in optical communication with the dynamic opacity optic and that receives transmitted light from the sample and produces a transmitted light signal based on an amount of transmitted light received; and an adjustment controller that receives the transmitted light signal, produces the adjustment signal, and communicates the adjustment signal to the dynamic opacity optic.

The present disclosure relates to optical systems for fluorescence-based imaging. An example optical system includes an image sensor. The image sensor is sensitive to at least a first wavelength of light and a second wavelength of light. The optical system also includes a light guiding layer optically coupled to the image sensor and a light source positioned to emit light into a side surface of the light guiding layer. The emitted light includes light at the first wavelength and the emitted light is transmitted in an in-plane direction in the light guiding layer. The optical system further includes a thin film filter and an output coupler optically coupled to the light guiding layer. At least a portion of the emitted light transmitted in an in-plane direction in the light guiding layer is coupled out of the light guiding layer in an out-of-plane direction via the output coupler.

An illuminator/collector assembly can deliver incident light to a sample and collect return light returning from the sample. A sensor can measure ray intensities as a function of ray position and ray angle for the collected return light. A ray selector can select a first subset of rays from the collected return light at the sensor that meet a first selection criterion. In some examples, the ray selector can aggregate ray intensities into bins, each bin corresponding to rays in the collected return light that traverse within the sample an estimated optical path length within a respective range of optical path lengths. A characterizer can determine a physical property of the sample, such as absorptivity, based on the ray intensities, ray positions, and ray angles for the first subset of rays. Accounting for variations in optical path length traversed within the sample can improve accuracy.

A light source may illuminate a scene with amplitude-modulated light. The scene may include fluorescent material. The amplitude modulation may be periodic, and the frequency of the amplitude modulation may be swept. During the sweep, a time-of-flight sensor may take measurements of light returning from the scene. A computer may calculate, for each pixel in the sensor, a vector of complex numbers. Each complex number in the vector may encode phase and amplitude of light incident at the pixel and may correspond to measurements taken at a given frequency in the sweep. A computer may, based on phase of the complex numbers for a pixel, calculate fluorescence lifetime and scene depth of a scene point that corresponds to the pixel.