A device may determine a calibration value for a spectrometer using light from a first light source; deactivate the first light source after determining the calibration value; perform measurement with regard to a sample based on the calibration value, wherein the measurement of the sample is performed using light from a second light source; determine that the calibration value is to be updated; and update the calibration value using the light from the first light source.

Tissue sample carriers for use in multispectral imaging are disclosed. In one aspect, a composition for fixing a tissue sample for multispectral imaging includes a carrier configured to protect and carry the tissue sample. The carrier has a first autofluorescence level when multispectral light strikes the tissue sample carried by the carrier. The composition further includes a pigment combined with the carrier and configured to reduce an autofluorescence property of the carrier such that the carrier has a second autofluorescence level when multispectral light strikes the tissue sample carried by the carrier. The second autofluorescence level is less than the first autofluorescence level.

A device may determine a calibration value for a spectrometer using light from a first light source; deactivate the first light source after determining the calibration value; perform measurement with regard to a sample based on the calibration value, wherein the measurement of the sample is performed using light from a second light source; determine that the calibration value is to be updated; and update the calibration value using the light from the first light source.

A method for measuring optical signal detector performance that includes directing light emitted from an optical signal detector onto a first non-fluorescent surface portion in a first detection zone of the optical signal detector. A first characteristic of light detected by a first sensor of the first optical signal detector is measured while the first non-fluorescent surface portion is in the first detection zone of the optical signal detector. Light emitted from the optical signal detector is directed into a first void in the first detection zone of the optical signal detector. A second characteristic of light detected by the first sensor of the optical signal detector is measured while the first void is in the first detection zone of the optical signal detector. And an operational performance status of the optical signal detector is determined based on at least one of the first characteristic and the second characteristic.

A device may determine a calibration value for a spectrometer using light from a first light source; deactivate the first light source after determining the calibration value; perform measurement with regard to a sample based on the calibration value, wherein the measurement of the sample is performed using light from a second light source; determine that the calibration value is to be updated; and update the calibration value using the light from the first light source.

In accordance with an example aspect of the present invention, there is provided a method of obtaining a calibrated measurement of a sample using an integrating cavity, comprising obtaining sample spectral information by using the integrating cavity with the sample placed inside the integrating cavity, obtaining cavity-characterizing spectral information generated by using the integrating cavity with a standard object, and obtaining a measurement result from the sample spectral information by employing a mathematical process that takes the cavity-characterizing spectral information as input.

This relates to systems and methods for measuring a concentration and type of substance in a sample at a sampling interface. The systems can include a light source, optics, one or more modulators, a reference, a detector, and a controller. The systems and methods disclosed can be capable of accounting for drift originating from the light source, one or more optics, and the detector by sharing one or more components between different measurement light paths. Additionally, the systems can be capable of differentiating between different types of drift and eliminating erroneous measurements due to stray light with the placement of one or more modulators between the light source and the sample or reference. Furthermore, the systems can be capable of detecting the substance along various locations and depths within the sample by mapping a detector pixel and a microoptics to the location and depth in the sample.

The present disclosure includes systems and methods for calibration of an optical sensor package, including setting an initial detection threshold of a detector, gradually increasing a power level of a signal generator that is in communication with a detector to cause a detected power at the detector to exceed the initial detection threshold, storing in a memory a first power level of the signal generator at which the detected power at the detector exceeds the initial detection threshold, and adjusting the initial detection threshold of the detector to an adjusted detection threshold to include a detection buffer amount within the adjusted detection threshold.

A method to correct signal light intensities measured by a detector of a detection unit in a laboratory instrument is presented. The detection unit comprises a light source, a sample plane comprising a sample holder configured to hold at least one sample vessel comprising a test sample to be illuminated, a reference light sensor, and the detector. Based on a basic light intensity of a newly manufactured light source and an initial light intensity measured by the reference light sensor the sensitivity of the reference light sensor can be determined. And signal light intensities measured by the detector can be corrected based on the determined sensitivity and subsequently measured reference light intensities of the reference light sensor in order to generate comparable test results.

In accordance with an example aspect of the present invention, there is provided a method of obtaining a calibrated measurement of a sample using an integrating cavity, comprising obtaining sample spectral information by using the integrating cavity with the sample placed inside the integrating cavity, obtaining cavity-characterizing spectral information generated by using the integrating cavity with a standard object, and obtaining a measurement result from the sample spectral information by employing a mathematical process that takes the cavity-characterizing spectral information as input.