The present disclosure relates to an ultrasonic device for real-time and nondestructive assessment of extracellular matrix stiffness, and the method of making and using the novel ultrasonic device.

An ultrasonic-image-construction apparatus can construct an ultrasonic-tomographic image of a thin, layer-structured target object to be measured relatively easily and highly accurately in a manner in which such layered structure is easily understood. An ultrasonic transducer of an ultrasonic-image-constructing apparatus transmits ultrasonic waves to the target object. A reference substance makes contact with a base substrate, with such ultrasonic waves being incident on the target object via the base substrate, then receives an impulse response of an ultrasonic waveform. A computing means performs calculation to estimate acoustic-physical-property distribution in consideration of the multiple-reflections influence based on normalized-impulse information obtained from impulse-response information of such ultrasonic waveform incident on the reference substance and from impulse-response information of such ultrasonic waveform incident on the target object. The image-construction means constructs acoustic-physical-property-image data based on acoustic-physical-property distribution in the depth direction obtained by computing means.

An ultrasound device, including a profile generator, an encoder configured to receive a profile signal from the profile generator, and an attenuator configured to receive a signal representing an output of an ultrasound sensor and coupled to the encoder to receive a control signal from the encoder, the attenuator including a plurality of attenuator stages, the attenuator configured to produce an output signal that is an attenuated version of the input signal.

In a first aspect, the present description relates to a system for non-invasively characterizing a heterogeneous medium using ultrasound, comprising at least one first array (10) of transducers configured to generate a series of incident ultrasound waves in a region of said heterogeneous medium and record the ultrasound waves which are backscattered by said region as a function of time, as well as a computing unit (42) which is coupled to said at least one first array of transducers and configured to: record an experimental reflection matrix defined between an emission basis at the input and the basis of the transducers at the output; determine, from said experimental reflection matrix, at least one first wideband reflection matrix defined in a focused base at the input and output; determine a first distortion matrix defined between said focused basis and an observation basis, said first distortion matrix resulting, directly or after a change of basis, from the term-by-term matrix product of said first wideband reflection matrix defined between said focused basis and an aberration correction basis, with the phase-conjugated matrix of a reference reflection matrix defined for a model medium, between the same bases; determine, from said first distortion matrix, at least one mapping of a physical parameter of said heterogeneous medium.

A device for supplying an ultrasonic transducer including a power interface configured to provide an analog power signal, called supply signal, to the ultrasonic transducer, and further including a delta-sigma modulator configured to produce a delta-sigma modulator of a sinusoidal signal, called drive signal, and provide a digital signal, called control signal, to control said power interface. Also an ultrasonic device powered by such a supply device, an ultrasonic head including such ultrasonic devices and an ultrasonic system including such an ultrasonic head.

Systems and methods for improving spectral-shift methods for calculating acoustic attenuation coefficients are disclosed. Systems, methods, and apparatuses for transmitting ultrasound pulse sequences for improved signal-to-noise outside the main passband of ultrasound transducers are disclosed. Systems, methods, and apparatuses for using the echoes from the transmitted pulse sequences to calculate the attenuation coefficient are disclosed.

The subject disclosure presents systems and computer-implemented methods for evaluating a tissue sample that has been removed from a subject. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The rate of change in the speed of the energy is correlated with the extent of diffusion. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. A time-of-flight of acoustic waves and rate of change thereof is monitored to determine an optimal time for soaking the tissue sample in a fixative.

A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

Method for ultrasonic characterization of a medium, comprising generating a series of incident ultrasonic waves, generating an experimental reflection matrix R.sub.ui(t) defined between the emission basis (i) as input and a reception basis (u) as output, and determining a focused reflection matrix RFoc(r.sub.in, r.sub.out, δt) of the medium between an input virtual transducer (TV.sub.in) calculated based on a focusing as input to the experimental reflection matrix and an output virtual transducer (TV.sub.out) calculated based on a focusing as output from the experimental reflection matrix, the responses of the output virtual transducer (TV.sub.out) being obtained at a time instant that is shifted by an additional delay δt relative to a time instant of the responses of the input virtual transducer (TV.sub.in).

A method for visualizing details in a sample including directing an excitation beam to an excitation location below a surface of the sample, to generate signals in the sample; directing an interrogation beam toward the excitation location of the sample; directing a signal enhancement beam to the sample, to raise a temperature of a portion of the sample by 5 Kelvin or less, compared to a temperature of the portion of the sample in absence of the signal enhancement beam; detecting a portion of the interrogation beam returning from the sample that is indicative of the generated signals.