The invention provides anti-ETEC adhesin protein antibodies and methods of using the same.

The present invention is directed to a microbial Cytometric Mock Community for use in flow cytometric analysis, the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio, wherein the at least three different microbial species are selected such that, when measured using flow cytometry, the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community, preferably the at least three different microbial species differ in relative DNA content, relative genomic GC-content, relative cell size, Gram +/− affiliation and/or capacity to form spores. The microbial Cytometric Mock Community shall serve as standardization means that will help ecologists, microbiologists, molecular biologists and flow cytometrists to work on a standardized basis to allow comparison and exchange of data.

Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filtering. The enzyme was found to have no significant effect on cell viability.

The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

The invention provides analytical methods for identifying and quantifying complex glycoconjugate compositions, in particular for the analysis of a glycoconjugate in a sample comprising at least 4 glycoconjugates.

Disclosed herein are example embodiments of a transformative sensor apparatus that is capable of detecting and quantifying the presence of a substance of interest such as a specified bacteria within a sample via changes in impedance exhibited by a detection electrode array. In an example embodiment, sensitivity is improved by including a focusing electrode array in a rampdown channel to focus a concentration of the substance of interest into a detection region. The focusing electrodes include an opposing pair of electrodes in a rampdown orientation. The focusing electrode may also include tilted thin film finger electrodes extending from the rampdown electrodes. In another example embodiment, trapping electrodes are positioned to trap a concentration of the substance of interest onto the detection electrode array.

The present subject matter provides glucose biosensors as well as compositions, devices, and methods comprising such biosensors.

Coliform detection is provided via the alpha (“α”) form of enzyme substrates. The α form can be mixed with a dry growth media suitable for target Coliforms and then applied as needed to a test card format, or then mixed with water and sterilized for use a broth applied to a membrane filter detection device, for example.

A biosensor having a core/shell nanocomposite of TiO2/rGO formed by hydrothermally coating reduced graphene oxide (rGO) flakes on titanate nanowires.

Binding agents able to disrupt bacterial biofilms of diverse origin are described, including monoclonal antibodies secreted by human B lymphocytes. Methods to prevent formation of or to dissolve biofilms with these binding agents are also described. Immunogens for eliciting antibodies to disrupt biofilms are also described.