Among the various aspects of the present disclosure is the provision of FimH inhibiting compositions and methods of use thereof. FimH inhibiting compositions that target and inhibit FimH, including monoclonal antibodies, are described. Methods of identifying FimH inhibiting antibodies are also described. Further, a method of treating bacterial infections, including urinary tract infections, is described.

Methods and devices are disclosed for rapid, multiplex molecular detection of diverse nucleic acid target molecules. The invention features an electrophoretic array with immobilized hydrogel microgel deposits. Each deposit comprises a three-dimensional, cross-linked polymer matrix containing an immobilized affinity-binding molecule and a porogen-derived pore network. This structure is configured for rapid molecular transport of nucleic acids (e.g., up to 800 bp), providing a localized environment for target capture, ligation of linear Rolling Circle Amplification (RCA) probes, and RCA. Target-specific components are anchored within distinct microgels for multiplexing. Electric fields enhance transport, reaction kinetics, and amplicon concentration. Detection is achieved in under 20 minutes. The specifically structured and fabricated microgels improve detection speed, sensitivity, and applicability to multiple different targets.

Provided herein are methods of analyzing a fecal sample including measuring the amount of ethanol produced by the fecal sample and measuring the presence, absence, or relative abundance of Ruminococcus gnavus, Escherichia coli, or Klebsiella pneumoniae in the fecal sample compared to a reference sample, and/or measuring the expression of genes associated with the heterolactic fermentation pathway, the mixed acid fermentation pathway, the ethanolamine utilization pathway, and/or acetylene degradation pathway. Also provided herein are methods of treating a condition associated with gut alcohol production in a subject including analyzing a fecal sample from a subject, determining the subject has a gut alcohol production condition, and administering to the subject an effective amount of a treatment comprising a probiotic with optional resistant starch, fecal microbiota transplantation (FMT), an antimicrobial agent, a microbial enzyme inhibitor, or a combination thereof.

A system of predicting sensitivity of Klebsiella against meropenem includes comprises a computer readable storage medium on which is stored a computer program. An Exp (k) power value calculation method is implemented when the computer program is executed by a processor. The Exp(k) power value calculation method comprises-includes following computing steps: S1: k value is calculated according to formula I, $k=-1.44+0.708\left(\frac{C1-0.252}{2.567}\right)-0.66\left(\frac{C2-0.981}{5.69}\right)+0.088\left(\frac{C3-0.952}{0.652}\right)+3.048\left(\frac{C4-0.469}{1.095}\right)-0.46\left(\frac{C5-0.296}{0.67}\right)$
S2: Exp(k) power value with natural constant e as base and k as exponent is calculated.

A method and kit for for determining of an effective concentration of an antimicrobial agent for inhibiting bacterial growth are provided. The method and kit involve the use of a first and second multiwell receptacle. A fluid sample containing or suspected to contain one or more microbial organisms is dispensed into the wells of the first multiwell receptacle containing a range of dilutions of an antimicrobial agent, growth medium and a bacteriophage to form assay mixtures in the wells. The assay mixtures are then transferred to the corresponding wells of the second multiwell receptacle for detection.