A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); eluting the bound biomolecules in the elution cell.

Systems and methods for detecting microbial toxins are disclosed. The system includes an imaging device operable to detect a luminescent signal and an analysis plate having a well to hold a sample containing the microbial toxin. The luminescence is created by a cell line expressing a product capable of reacting directly or indirectly with the microbial toxin to produce the luminescent signal. The signal is processed via an image processing system operable to receive the luminescent signal detected by the imaging device and convert the luminescent signal to a quantitative measurement correlated to an amount of the microbial toxin present in the sample.

Populations of polypeptide variants based on a common scaffold, each polypeptide in the population comprising the scaffold amino acid sequence EXXXAXXEIX XLPNLTXXQX XAFIXKLXDD PSQSSELLSE AKKLNDSQ (SEQ ID NO: 1) or AKYAKEXXXAXX EIXXLPNLTX XQXXAFIXKL XDDPSQSSEL LSEAKKLNDS Q (SEQ ID NO: 2), wherein each X individually corresponds to an amino acid residue which is varied in the population are disclosed. Also populations of polynucleotides, wherein each member encodes a member of a polypeptide population are disclosed. Furthermore, combinations of such polypeptide populations and such polynucleotide populations are disclosed, wherein each member of polypeptide population is physically or spatially associated with the polynucleotide encoding that member via means for genotype-phenotype coupling.

The present invention relates to proteins and nucleic acids derived from Staphylococcus aureus as well as therapeutic and diagnostic uses of the proteins and nucleic acids.

An immunochromatographic method for detecting a specific substance contained in milk, which comprises (1) the step of contacting the milk with a test strip having a first part retaining a labeled first antibody directed to the specific substance or the specific substance that is labeled, a second part disposed downstream from the first part, on which a second antibody directed to the specific substance is immobilized, and a third part disposed upstream from the first part or the second part and having voids enabling removal of milk fat globules contained in the milk, at the third part or a further upstream part, and (2) the step of flowing the milk up to the second part or a further downstream part to obtain a detectable signal of the label at the second part or a further downstream part.

The present disclosure provides materials and methods for cell-free expression of epitopes for immunotherapy applications. In particular, the present disclosure provides materials and methods for expressing concatenated epitopes using a cell-free protein synthesis platform for high throughput, large scale, and unbiased epitope screening and the generation of multi-epitope vaccines.

The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacterium of mastitis is a staphylococcus or not by using milk of a livestock animal. There is provided a method for lysing a staphylococcus, which comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of ampholytic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse a staphylococcus existing in the milk. The lytic enzyme is preferably lysostaphin.

The present invention provides a lateral flow device for detecting lipoteichoic acid (LT A) as a Gram-positive bacteria identifier in a milk sample of an animal. The device comprises: a) a strip formed of a material enabling capillary flow of fluid along a portion of the strip; b) a sample pad located proximal to one end of the strip for receiving the milk sample, c) a conjugate pad located in the strip so that in operation the sample flows under capillary action through the strip from the sample pad to the conjugate pad and mobilizes a conjugate contained in the conjugate pad, the conjugate comprising an anti-LTA antibody that has been conjugated to a detection agent, d) a test line Test Die comprising an anti-LTA antibody immobilized within the strip along a band located substantially perpendicular to the direction of the sample flow along the strip so that when a formed complex comprised of the mobilized anti-LTA antibody conjugate and the LT A in the sample contacts the immobilized anti-LTA antibody in the test line the presence of LT A in the sample is indicated by a visible color change.

The present invention relates to the field of biomedicine, and provides an isolated antigen-binding protein and a derived protein thereof, which can specifically bind to Staphylococcus aureus toxin and neutralize the biological activity thereof. Also provided is a use of the isolated antigen-binding protein and derived protein thereof in the field of treating and/or preventing Staphylococcus aureus infection.

The present invention relates to an immunogenic composition comprising at least one Staphylococcus aureus antigen, wherein said antigen is a polypeptide having at least 80% identity with the SdrH-like polypeptide of SEQ ID NO: 8, Nuc of SEQ ID

NO: 4, or LukG of SEQ ID NO: 12, an immunotherapeutic composition comprising a polyclonal antibody which selectively binds to at least one of said antigens, and an in vitro method of identifying an antigen conferring protection against disease caused by S. aureus in a subject.