A system for early detection and treatment of sepsis, comprising a fluid transfer device having an inlet and an outlet. The inlet is configured to receive a flow of bodily fluid. A flow-based assay device is configured to be coupled to the fluid transfer device. A portion of the flow-based assay device engages the outlet to allow a portion of a first volume of the bodily fluid to be transferred to the flow-based assay device. The flow-based assay device is configured to detect at least one sepsis-associated biomarker. Processing circuitry is configured to receive data from the flow-based assay device, apply a computational model to the received data, generate a sepsis probability score based on an output of the applied computational model, and when the sepsis probability score exceeds a sepsis-associated threshold, alert a care provider to initiate corresponding treatment.

The invention is directed to non-invasive methods of detecting gastric cancer in an individual in need thereof comprising determining an expression level of inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) in the individual and comparing the expression level to a control, wherein an increase in the expression level of ITIH3 in the individual compared to the control is indicative of gastric cancer in the individual.

The invention provides a method for screening for and detection of solid organ graft rejection in a subject that comprises assaying a patient sample of plasma, serum or blood from the subject for a protein marker identified herein. An elevated or reduced amount of marker present in the patient sample compared to a control sample is indicative of rejection, and identifies subjects in need of biopsy or modified treatment. The method can be used to screen for patients in danger of transplant rejection without having to undergo more costly, risky and invasive biopsy procedures.

Anti-AS-SPIK antibodies are disclosed, along with methods of making such antibodies, compositions, including pharmaceutical compositions, comprising such antibodies, and their use to diagnose and/or treat disorders characterized by the expression of AS-SPIK (e.g., liver cancer). Diagnostic methods and kits comprising the anti-AS-SPIK antibodies are also disclosed.

A2M polypeptide compositions containing a non-natural bait region are disclosed. Methods of producing wild-type and variant A2M polypeptides and polynucleotides containing a non-natural bait region are also disclosed. The bait regions of the variant A2M polypeptides demonstrate enhanced protease inhibitory characteristics compared to wild-type A2M. Variant A2M polypeptides that demonstrate longer half-lives upon administration to an organism compared to wild-type A2M are disclosed. The A2M compositions are useful in treating a number of diseases and conditions including inflammation, chronic wounds, and diseases with a pathology associated with proteases.

Proposed are a biomarker for diagnosis of advanced gastric cancer and a use thereof. Specifically, by measuring the level of epithelial cells in which serine peptidase inhibitor, Kunitz type 2 (SPINT2), or post-glycosylphosphatidylinositol (GPI) attachment to proteins 3 (PGAP3) is highly expressed, from a biological sample of a patient, the efficiency in diagnosis of gastric cancer was confirmed to be excellent. Thus, the level of the biomarker can be measured and utilized for prevention, improvement, and/or treatment of gastric cancer.

Featured are a foodstuff containing an inter-alpha inhibitor protein (IIp) (e.g., II, PI, a heavy chain (e.g., H1, H2, H3, H4, and/or H5), a light chain (e.g., bikunin), or a combination thereof), methods of preparing a foodstuff containing an IIp, and methods of treating and/or reducing the likelihood of developing disease or condition (e.g., a disease or condition characterized by inflammation and/or low levels of an IIp) in a subject in need thereof by administering a composition or a foodstuff containing an IIp. Also featured are methods of purifying an IIp (e.g., II, PI, a heavy chain (e.g., H1, H2, H3, H4, and/or H5), a light chain (e.g., bikunin), or a combination thereof) from milk.