A process for determining the viability of a biological indicator includes exposing the biological indicator to a viability detection medium, the biological indicator including test microorganisms, the exposing the biological indicator to the viability detection medium producing a gaseous reaction product when one or more of the test microorganisms are viable. The presence or absence of the gaseous reaction product produced by the biological indicator combined with the viability detection medium is detected with a sensing device, the sensing device comprising a resistive sensor, wherein the presence of the gaseous reaction product indicates the presence of viable test microorganisms and the absence of the gaseous reaction product indicates the absence of viable test microorganisms. A sterilization detection device includes a container configured to contain the biological indicator, a viability detection medium, and the sensing device.

A chromogenic absorbent material for an animal litter includes an oxidizing agent responsive to peroxidatic/pseudoperoxidatic activity in an animal excretion or a first catalytic compound generating the oxidizing agent in situ. The material also includes a chromogenic indicator being chromogenically responsive to the oxidizing activity of the oxidizing agent, and an absorptive material which is porous, for absorbing the animal excretion. The absorptive material includes a water-absorbing polysaccharide providing absorptive properties to the chromogenic absorbent material; and may also include a second polysaccharide and a superabsorbent polymer. The material may be obtained in the form of particles having a low density and a high porosity, and is usable in conjunction with an animal litter for detecting various diseases in animals.

A method for quantifying cardiolipin in a sample, comprises the steps of: (1) treating the sample with phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand; and a kit for quantifying cardiolipin comprises phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase.

The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein cross-linker) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.

A chromogenic absorbent material for an animal litter includes an oxidizing agent responsive to peroxidatic/pseudoperoxidatic activity in an animal excretion or a first catalytic compound generating the oxidizing agent in situ. The material also includes a chromogenic indicator being chromogenically responsive to the oxidizing activity of the oxidizing agent, and an absorptive material which is porous, for absorbing the animal excretion. The absorptive material includes a water-absorbing polysaccharide providing absorptive properties to the chromogenic absorbent material; and may also include a second polysaccharide and a superabsorbent polymer. The material may be obtained in the form of particles having a low density and a high porosity, and is usable in conjunction with an animal litter for detecting various diseases in animals.

Provided herein are methods for detecting a target moiety in a biological sample suspected of containing the target moiety, by incubating the biological sample with an antibody and a fusion polypeptide, the fusion polypeptide comprising (i) an immunoglobulin-binding polypeptide and (ii) a reporting species.

A method for labeling concentration density differentials of an analyte in a biological sample is provided. The method may including the steps of: binding an enzyme to an analyte contained in a sample, the enzyme capable of acting on at least two chromogens; incubating the sample with a first chromogen for a first time period to generate a first color chromogen-enzyme product, the first color chromogen-enzyme product reflecting light observable as a first color; and incubating the sample with a second chromogen for a second time period to generate a second color chromogen-enzyme product, the second color chromogen-enzyme product reflecting light observable as second color. A combination of the light observable as the first color and the light observable as the second color may be observable as a third color, and each color may describe a different analyte density in the biological sample.

[PROBLEM] To provide a monoclonal antibody against a biomarker which shows high specificity and can be effectively used in detection and diagnosis of various lesions relevant to various kinds of carcinomas and foci of necrosis, and so forth. [MEANS] A monoclonal antibody against a necrosis marker consisting the following amino acid sequence: (1) the amino acid sequence of SEQ ID NO: 1, or (2) an amino acid sequence having substitution, deletion and/or insertion of one or several amino acid residues in the amino acid sequence of (1) or sharing a homology of 90% or more with the amino acid sequence of (1), and showing the same function, activity or property as that of the amino acid sequence of (1) as a protein.

A method of preparing nucleic acid-inorganic hybrid nanoflowers is described, in which a nucleic acid is allowed to react with a solution of a metal ion-containing compound at room temperature, thereby forming a complex between the metal ion and the nitrogen atom of an amide bond or amine group present in the nucleic acid. Organic-inorganic hybrid nanoflower structures thus may be synthesized using nucleic acid in a simple manner under an environmentally friendly condition without any toxic chemical substance. The produced organic-inorganic hybrid nanoflower structures exhibit a high DNA encapsulation yield, nuclease resistance, and significantly increased peroxidase activity. These nanoflower structures may be widely used as gene therapy carriers and in biosensing technology.

The invention provides compositions and methods for determining cardiodiabetes status in a subject. The invention also provides compositions and methods for treating a subject experiencing cardiodiabetes.