The purpose of the present invention is to provide a method for evaluating the clogging of a filtration membrane with a protein-containing solution, including the steps of: a) passing a protein-containing solution through a filtration membrane, b) after the step a), obtaining a filtration membrane cross-section from the filtration membrane, c) treating the protein-containing solution before the step a), the filtration membrane before the step b), or the filtration membrane cross-section after the step b) with at least one stain specific to a protein aggregate, and d) confirming the presence of the protein aggregate in the filtration membrane cross-section.

The invention relates to a combination or panel of HIE-relevant protein and/or microRNA (miRNA) biomarkers that are released from injured tissues into biofluids such as blood in HIE, and their use as markers for detection of HIE. A selected panel of blood-based protein and/or miRNA HIE biomarkers that are measured at more than one time interval can aid in the diagnosis of HIE severity, and to determine the prognosis of poor versus good cognitive or overall patient outcome.

The present invention relates to efficient methods for providing antigen-specific lymphoid cells. These lymphoid cells may be used to provide antigen specific T cell receptors having a defined MHC restriction and to identify immunologically relevant T cell epitopes. Furthermore, the present invention relates to antigen-specific T cell receptors and T cell epitopes and their use in immunotherapy.

The present disclosure relates to anti-CD39 antibodies, and antigen binding portions thereof and their use in treating cancer.

The present disclosure relates, in general, to methods for screening for modulators of IFNβ activity using a GLuc/SEAP dual reporter system.

Disclosed herein are methods, and kits for use in said methods, that aid in the diagnosis and evaluation of a subject that has sustained an orthopedic injury and sustained or may have sustained an injury to the head, such as mild traumatic brain injury (TBI), using ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), glial fibrillary acidic protein (GFAP), or a combination thereof. Also disclosed herein are methods, and kits for use in said methods, that aid in determining whether a subject that has sustained an orthopedic injury and sustained or may have sustained an injury to the head would benefit from and thus receive an imaging procedure, such as MRI or head computerized tomography (CT) scan based on the levels of GFAP and/or UCH-L1. These methods involve detecting levels and changes in levels of GFAP and/or UCH-L1 in biological samples taken from a subject at time points within 48 hours after the subject has sustained or may have sustained an injury to the head.

Methods of diagnosing infections are disclosed. In one embodiment, the method comprises measuring the amount of each of the polypeptides TRAIL, CRP, IP10 and at least one additional polypeptide selected from the group consisting of IL-6 and PCT.

The invention relates to senescent cell biomarkers and the uses thereof. The invention also extends to methods and kits for detecting senescence, and drug conjugates and pharmaceutical compositions for killing senescent cells.

Provided is a ultra-high-sensitivity assay in which the assay can be made on a commonly used assay apparatus such as an absorptiometer and a plate reader or with naked eyes. The high-sensitivity assay in which the assay can be made on a commonly used assay apparatus or with naked eyes can be provided by combining an enzyme cycling method using thio-NAD(P) as a coenzyme, a labeling enzyme and a substrate for the labeling enzyme optimally, and by amplifying thio-NAD(P)H, which is a signaling substance, exponentially and then quantifying the thio-NAD(P)H colorimetrically.

The present invention relates to a method of detecting the presence of an antibody to an avian influenza hemagglutinin antigen in a sample from a subject, wherein the antibody binds to an epitope of an avian influenza hemagglutinin antigen, comprising the steps of cloning a nucleic acid encoding an avian influenza antigen into a vector, infiltrating a plant cell with the vector, recovering the antigen from the plant, contacting the sample with the antigen, and detecting the formation of an antibody-antigen complex, wherein the antibody-antigen complex comprises antibodies in the sample bound to the antigen and wherein the formation of an antibody-antigen complex confirms that the subject has been exposed to an avian influenza hemagglutinin antigen. The present invention also relates to a device for assaying the presence of an antibody to avian influenza hemagglutinin antigen in a sample from a subject.