According to a first aspect, the present disclosure relates to a digital holography device (100) for full-field blood flow imaging of ocular vessels of a field of view of a layer (11) of the eye (10). The device comprises an optical source (101) configured for the generation of an illuminating beam (Eobj) and a reference beam (E.sub.LO), and a detector (135) configured to acquire a plurality of interferograms (I(x,y,t)) wherein an interferogram is defined as the signal resulting from the interference between the said reference beam (E.sub.LO) and a part of said illuminating beam (Eobj) that is backscattered from said layer (11). The device further comprises a processing unit (150) configured for processing said plurality of interferograms, (I(x,y,t)), wherein said processing comprises: the calculation (202), for each interferogram, of a hologram (H(x,y,t)), resulting in a first plurality of holograms; the selection (203), in sequential time windows, (tw), of second pluralities of holograms; the calculation (204), for each said second plurality of holograms, of a Doppler power spectrum (S(x,y,f)); the calculation (205), based on said Doppler power spectrum, of at least a first Doppler image thus generating at least a first plurality of Doppler images; the processing of each first Doppler image, wherein said processing comprises the devignetting (206) of said first Doppler image, resulting in a devignetted first Doppler image; the normalization (207) of said devignetted first Doppler image based on a spatial average of an intensity of said first Doppler image, resulting in a normalized first Doppler image; and the subtraction (208), from said normalized first Doppler image, of said spatial average of said intensity of said first Doppler image, resulting in a corrected first Doppler image.

Systems and methods for enhancing quality of a flow image of a sample of a subject are provided. The method comprises acquiring a first flow image from a plurality of first OMAG scans of the sample, and acquiring a structure image from a second OMAG scan of the sample. Data based on pixel intensity values from the flow image and pixel intensity values from the structure image are then plotted onto a graph and from the graph, may be differentiated into a first data group representing static structure signals and a second data group representing flow signals. The method then includes suppressing pixels in the flow image corresponding to the first data group. The flow signal may also be multiplied by a weighting factor to suppress artifacts in the image.

Methods and systems for measuring hemoglobin concentration (c.sub.Hb). A retinal tissue having blood vessels is illuminated by light including two isosbestic wavelengths (λ.sub.1,λ.sub.2). The isosbestic wavelengths(λ.sub.1,λ.sub.2) correspond to isosbestic points where oxy- and deoxyhemoglobin (HbIO.sub.2,Hb) have the same molar extinction coefficient. Measurements are collected of a plurality of backscattered reflection intensities (I(χ.sub.b,λ.sub.1), I(x.sub.t,λ.sub.1), I(χ.sub.b,λ.sub.2), I(x.sub.x,X.sub.2)). Preferably, the measurements are spectrally resolved at least for the two isosbestic wavelengths (λ.sub.1,λ.sub.2) and position-resolved for at least a blood vessel location (x.sub.b) coinciding with a selected blood vessel and a tissue location (x.sub.t) coinciding with the retinal tissue without blood vessel. The hemoglobin concentration (c.sub.Hb) can be calculated based on a combination of the measurements at the two isosbestic wavelengths (λ.sub.t,λ.sub.2).

System and method are presented for use in inspecting a biological tissue. According to this technique, a predetermined location within the biological tissue is illuminated by an illumination pattern comprising a predetermined exciting wavelength range selected to substantially concurrently induce two or more auto-fluorescent responses of two or more predetermined different biological substances of types naturally existing in biological tissues to enable detection of a combined spectral response of the biological tissue to said illumination pattern. Upon identifying in said detected combined spectral response emission wavelengths of said two or more substances, output data is generated being indicative of simultaneous existence on said location of the combination of said two or more predetermined different biological substances, which provides direct indication about said pathological condition.

A visualization of choroidal blood vessel size is created. A vortex vein position is detected from a choroidal vascular image in which choroidal blood vessels have been visualized. Image processing is employed on the choroidal vascular image to extract first size choroidal blood vessels of a first size and to extract second size choroidal blood vessels of a second size different to the first size from the choroidal vascular image. A size analysis fundus image is generated in which a rectangular frame indicating the vortex vein position is displayed superimposed on the choroidal vascular image, and in which the first size choroidal blood vessels are displayed in red and the second size choroidal blood vessels are displayed in blue.

An optical coherence tomographic device of polarization-sensitive type may include: an image capturing unit configured to capture a tomographic image of a subject eye, and a display unit configured to display the tomographic image captured by the image capturing unit. The tomographic image may include at least two images selected from a group consisting of: an image showing a tissue in the subject eye by scattering intensity, an image showing a melanin distribution in the subject eye, an image showing a fiber density in the subject eye, an image showing a fiber direction in the subject eye, and an image showing a blood flow in the subject eye. The display unit may be configured to display the at least two images at a same position and on a same cross section such that the at least two images are superimposed on each other.

The invention relates to a device and a method, by which the intraocular pressure in a patient's eye is changed by artificially applying a variable stimulation pressure causing, upon reaching specific intraocular pressure values, the presence of characteristic measurement criteria in the retina of the eye, which allow global and local retinal blood pressure values to be derived from the intraocular pressure value. Based on the retinal blood pressure values, which are determined online or preferably offline, local retinal perfusion pressure values (rPP) can be computed and represented in an image of the retina as a pressure mapping image.

A non perfusion area is detected from a fundus image. A fundus image is acquired, a first non perfusion area in a first region of the fundus is extracted from the fundus image, and a second non perfusion area in a second region of the fundus is extracted from the fundus image.

An example eye imaging device can include: a camera configured to capture fundus images of an eye of a patient; and a stimuli to configured to stimulate the patient and assess a disease state or a mental state of the patient. Another example eye imaging device can include: a camera configured to capture fundus images of an eye of a patient; and a physical barrier surrounding at least a portion of the eye imaging device to minimize exposure of the eye imaging device.

An example system for microvascular assessment of a patient can include: a fundus imaging device including: a camera configured to capture one or more images of an eye of the patient; and at least one light source; and a microvascular assessment computing device including: a processor; and memory encoding instructions which, when executed by the processor, cause the system to: activate the light source to direct light at a fundus of the eye of the patient; capture, with the camera, one or more images of the fundus of the patient, the fundus comprising a plurality of blood vessels; and analyze with the microvascular assessment computing device, the one or more images to determine a microvasculature health index for the patient.