A device for NMR spectroscopy includes a magnet arrangement, configured to produce a magnetic probe field within a magnet field of view external to the magnet arrangement. In a embodiment, the device includes a coil arrangement, configured to generate an electromagnetic excitation field within a coil field of view and a controller, configured to control the coil arrangement. The device includes a magnet adjustment arrangement, configured and arranged to modify at least one parameter of the magnet arrangement to change a spatial position of the magnet field of view.

The present invention relates to novel organic dinitroxide biradical compounds and their use as polarizing agents, in particular, in the techniques of Nuclear Magnetic Resonance (NMR) of solids or liquid samples and medical imaging.

The present disclosure provides methods for measuring interactions between a ligand and a protein, comprising hyperpolarizing a ligand in a solvent using para-hydrogen to form a first solution; transferring the first solution to a detector; mixing the first solution with a protein solution, the protein solution having one or more ligands of interest therein; and determining interactions of the hyperpolarized ligand with the one or more ligands of interest by observing a change in an NMR signal of the hyperpolarized ligand. The ligand can include one or more sites for hyperpolarization by parahydrogen, and one or more binding sites for interaction with the protein.

An article of manufacture for providing an onboard vehicle recharging environment according to the present invention is disclosed. A Continuous Onboard Recharging Environment (CORE) translates mechanical rotational energy obtained from the rotating axles of a vehicle to a form of sufficient voltage and load amperage to facilitate the charging of an Electric Vehicle's battery system while the vehicle is in operation, thus reducing or removing the need for external charging.

A method of diagnosing or monitoring a patient suffering from cancer, the method comprising: administering a pharmaceutical composition comprising an effective amount of an active agent, wherein the active agent is [1-.sup.13C] N-acetyl cysteine, a deuterated derivative thereof, a pharmaceutically acceptable salt of any of the foregoing thereof, or a combination thereof, together with a pharmaceutically acceptable carrier to the patient; and diagnosing or monitoring the patient by hyperpolarized .sup.13C-MRI. Also disclosed is a method of synthesizing [1-.sup.13C] N-acetyl cysteine or a deuterated derivative thereof.

A method for preparing an NMR material, comprising generating parahydrogen in gas or liquid form at a first location; transporting the parahydrogen away from the first location; mixing a precursor compound including a metabolite component with a catalyst for hydrogenation; hydrogenating the precursor compound using the parahydrogen; transferring polarization in the precursor compound to a nuclear spin of the metabolite component; cleaving a side arm of the precursor compound in a chemical reaction, with the metabolite molecule being one of the products of the reaction; separating the metabolite molecule from the catalyst for hydrogenation and other products of the reaction; and generating metabolite molecules for use in an MRI scanner by extracting a sample of the metabolite molecule having at least 5% polarization.

In one aspect, the disclosure relates to a compact membrane reactor for parahydrogen induced hyperpolarization, the reactor including an inner tube constructed of a semipermeable membrane, an outer tube surrounding the inner tube, and a means for controlling the magnetic field surrounding the outer tube. In some aspects, multiple compact membrane reactors can be arranged in parallel. In other aspects, the compact membrane reactor is equipped with a mechanism for magnetic field control. Also disclosed are fluid handling systems and sample preparation systems comprising the compact membrane reactors disclosed herein. The compact membrane reactor can be operated in a batch mode, a stopped-flow mode, or a continuous flow mode and can be configured to work with existing NMR spectrometers and MRI instruments.

A device(100a(i), 100a(ii), 100a(iii), 100a(iv), 200 for cis-tracing polarization of .sup.12C isotope-based magnetic resource imaging contrast agents, comprising one or more diamond material structures(120(i), 120(ii), 120(iii), 120(iv), 210, 220) and one or more channels(105a(i), 105a (ii), 105a (iii), 105a (iv), 205) provided adjacent to the diamond material structures 120(i), 120(ii), 120(iii), 120(iv), 210, 220); the diamond material structures 120(i), 120(ii), 120(iii), 120(iv), 210, 220) provide a source of negatively charged nitrogen vacancy celles for polarization of a .sup.13C iso-type-based magnetic measure imaging contrast agesi disguised in one or more channels(105a (i), 105a (ii), 105a (iii), 105a (iv), 205) and cis diamond material structure(120(i), 120(ii), 120(iii), 120(iv), 210, 220) provides a light guide for light for excitation of nitrogen vacancy celles for polarization of .sup.13C isotope-based magnetic resource imaging contrast agent.

Disclosed are methods for performing dynamic nuclear polarization using the polarizing agents described herein. In general, the methods involve (a) providing a frozen sample in a magnetic field, wherein the frozen sample includes a polarizing agent described herein and an analyte with at least one spin half nucleus; (b) polarizing the at least one spin half nucleus of the analyte by irradiating the frozen sample with radiation having a frequency that excites electron spin transitions in the polarizing agent; (c) optionally melting the sample to produce a molten sample; and (d) detecting nuclear spin transitions in the at least one spin half nucleus of the analyte in the frozen or molten sample. In certain embodiments, the polarizing agents can be peptide-based. In these embodiments, the polarizing agents can be readily prepared by solid-phase peptide synthesis.

The present invention teaches devices and methods for hyperpolarization by parahydrogen induced polarization. The invention teaches several significant improvements over previous designs, including a heating block, an enhanced solenoid component, and pinch valves and tubing that provide a sterile environment for the sample. All of these advancements can be accomplished while keeping costs to produce the device relatively low.