A scanning microscope for z-stack acquisition may include, a stage to hold a sample, an illumination source to illuminate the sample, and an image capture device configured to capture multiple images of the sample within a field of view of the image capture device. The microscope may also include a lateral actuator for changing a relative lateral position between the image capture device and an imaged portion of the sample within the field of view of the image capture device for each of the images, and a focus actuator configured to adjust a focal distance between the sample and the image capture device between each of the images. The microscope may further include a processor connected to the lateral actuator and the focus actuator to move the sample laterally relative to the field of view and capture an area of the sample for each of multiple movement paths.

A high-illumination numerical aperture-based large field-of-view high-resolution microimaging device, and a method for iterative reconstruction, the device comprising an LED array (1), a stage (2), a condenser (3), a microscopic objective (5), a tube lens (6), and a camera (7), the LED array (1) being arranged on the forward focal plane of the condenser (3). Light emitted by the i-th lit LED unit (8) of the LED array (1) passes through the condenser (3) and converges to become parallel light illuminating a specimen (4) to be examined, which is placed on the stage (2); part of the diffracted light passing through the specimen (4) is collected by the microscopic objective (5), converged by the tube lens (6), and reaches the imaging plane of the camera (7), forming an intensity image recorded by the camera (1). The present device and method ensure controllable programming of the illumination direction, while also ensuring an illumination-numerical-aperture up to 1.20 and thus achieving a reconstruction resolution up to 0.15 μm.

Method and system for trans-illumination imaging. In an exemplary method, a sample is irradiated in a well. The sample may include biological cells and a liquid or semi-solid medium that forms a meniscus. The step of irradiating may be performed with an array of light sources generating light of respective light beams that are incident on the meniscus at different orientations from one another. One or more images may be detected. A proportional contribution of light from two or more subsets of the light sources to the one or more images may be controlled by differential energization of the two or more subsets relative to one another to compensate for refraction by the meniscus and improve contrast.

The present invention concerns an image conversion module (09) that comprises an optical interface (10) for establishing an optical path (07). The image conversion module (09) further comprises a beam splitting element (13) on the optical path (07). The beam splitting element (13) is configured for splitting a beam entering the optical interface (10) on the optical path (07) into a first optical subpath (14) and a second optical subpath (16). The image conversion module (09) further comprises a microelectromechanical optical system (17) that is configured for enhancing a depth of field on the first optical subpath (14) that is directed to a first optoelectronic submodule (21), which comprises an image sensor (22). The image conversion module (09) further comprises a second optoelectronic submodule (24) that comprises an electronic sensor (26) on the second optical subpath (16). The second optoelectronic submodule (24) is configured for acquiring additional data on the sample (02). Furthermore, the present invention concerns a method for applying said image conversion module (09).

Provided are an apparatus and a method that can acquire a shaded three-dimensional image having a high contrast for a thick cell culture specimen. Provided are an apparatus and a method that observe a biological sample accommodated in a container by a modulation contrast method using a near-infrared wavelength, at least the bottom surface of the container being formed of a plastic raw material.

An optical detection device having a light detection device and a light emission device is arranged such that the light detection side of the light detection device is optically coupled to a light emission side of a light source array of the light emission device via an examination region. The light detection device generates an electrical signal n response to light that reaches the light detection side. The light source array includes a plurality of separately actuatable electric light sources which are arranged in a matrix structure or two dimensional geometric arrangement. The object to be examined can be arranged in a desired fashion, and the light emitted by the light sources radiates via the examination region on the light detection side of the light detection device. An optical reduction is system is arranged in the beam path from the light emission side to the examination region and is configured to demagnify the light pattern which is emitted by the light sources. Thus, the examination region is irradiated by a light pattern that has been demagnified with respect to the light pattern emitted.

An observation device including: a stage having a transparent pedestal surface; an illumination system under the pedestal surface, the illumination system emitting illumination light toward the pedestal surface; and an object system under the pedestal surface for capturing transmission light generated from the illumination light, reflected off a reflection surface and transmitted through a sample. Wherein a first light path in the illumination system is different from a second light path in the object system, in a first pupil surface of the illumination system, the illumination system generates the illumination light by restricting light in a first transparent region, in a second pupil surface of the object system, the object system restricts the transmission light in a second transparent region, and the second transparent region has first and second subregions with different transmittance, the second subregion located between the first subregion and an edge of the second transparent region.

A system for the simultaneous videographic or photographic acquisition of images, in particular of samples in a plurality of sample chambers of a sample plate, preferably a microtiter plate, includes an array of microscopes having mutually parallel optical axes, wherein each microscope includes an imaging chip and an objective. The imaging chips are attached to a carrier board as an array of columns and rows. An electronics unit for processing image data for all the microscopes is associated with the carrier board.

An image acquisition device includes: a stage on which a sample is placed; a drive unit; a first irradiation optical system; a second irradiation optical system; a beam splitter; a pupil dividing element; a first imaging lens; a first imaging element; an analysis unit; a control unit; a second imaging lens; and a second imaging element, a first irradiation light irradiation range captured by the first irradiation optical system includes a second imaging region captured by the second imaging element, the second imaging region is located behind a first imaging region in a scanning direction, and the control unit controls a focus position of an objective lens based on focus information before capturing the second imaging region by the second imaging element.

Disclosed herein is a smartphone microscope hardware for facilitating diagnosing microscopic objects in a sample of an object, in accordance with some embodiments. Accordingly, the smartphone microscope hardware may include a smartphone case, a magnifier, a glass slide, and a light source. Further, the smartphone case is configured to interface with a smartphone. Further, the magnifier is attached to the smartphone case. Further, the glass slide is configured for receiving the sample. Further, the camera is configurable for capturing an image of the sample. Further, the magnifier is configured for magnifying the image prior to the capturing. Further, a processing device of the smartphone is configurable for analyzing the image, identifying a microscopic object, and generating a notification. Further, a display device of the smartphone is configurable for displaying the image and the notification. Further, the light source is disposed adjacent to a second side of the glass slide.