Described herein are systems and methods for assessing a biological sample. The methods include: characterizing a speckled pattern to be applied by a diffuser; positioning a biological sample relative to at least one coherent light source such that at least one coherent light source illuminates the biological sample; diffusing light produced by the at least one coherent light source; capturing a plurality of illuminated images with the embedded speckle pattern of the biological sample based on the diffused light; iteratively reconstructing the plurality of speckled illuminated images of the biological sample to recover an image stack of reconstructed images; stitching together each image in the image stack to create a whole slide image, wherein each image of the image stack at least partially overlaps with a neighboring image; and identifying one or more features of the biological sample. The methods may be performed by a near-field Fourier Ptychographic system.

An illuminator includes a light source and an illumination optical system for causing light emitted from the light source to be incident on a sample surface where an imaging object is present. The illumination optical system has an optical axis coaxial with that of an imaging optical system. An image of the light source is formed between the illumination optical system and the imaging optical system. A holder arranges the sample surface between the light source image and the imaging optical system.

A multi-mode illumination system, including: a first illumination module; a second illumination module; and a third illumination module, as disclosed herein.

An object transfer function for a sample object is determined on the basis of a reference measurement. Subsequently, an optimization is carried out in order to find an optimized illumination geometry on the basis of the object transfer function and an optical transfer function for an optical unit.

A kit (10) includes a light source (12) and an optical system (14) equipped with a lens assembly (25) defining a magnification optical axis (X-X). A frame (16) is crossable by the light generated by the light source (12). The frame (16) is configured for supporting a sample holder (H), a portable electronic apparatus (S) equipped with an image acquisition device (C), and the optical system (14), which are interposable between the sample holder (H) and the image acquisition device (C). The optical system (14) is configured for being movable in a guided manner on the frame (16), to allow aligning the optical axis (X-X) with the image acquisition device (C). A carrying body (18) is configured for receiving in abutment the frame (16) and housing the light source (12) directing light towards the optical system (14) through the frame (16).

The invention relates to a method for microscopic evaluation (120) of a sample (2), in particular at least one uncolored object or cell sample (2), in an optical detection system (1), where the following steps are performed: providing at least two different detection information (110) about the sample (2), in particular by the detection system (1),
performing an evaluation (120) of the detection information (110), in particular by an analysis means (60), on the basis of machine-learned transfer information (200), in order to determine result information (140) about the sample (2),
the transfer information (200) being trained for a different detection parameterization of the detection information (110), in which the detection information (110) differs from one another in terms of at least one illumination parameter of the detection system (1), in particular in terms of polarization and/or color coding.

Described herein are systems and methods for assessing a biological sample. The methods include: characterizing a speckled pattern to be applied by a diffuser; positioning a biological sample relative to at least one coherent light source such that at least one coherent light source illuminates the biological sample; diffusing light produced by the at least one coherent light source; capturing a plurality of illuminated images with the embedded speckle pattern of the biological sample based on the diffused light; iteratively reconstructing the plurality of speckled illuminated images of the biological sample to recover an image stack of reconstructed images; stitching together each image in the image stack to create a whole slide image, wherein each image of the image stack at least partially overlaps with a neighboring image; and identifying one or more features of the biological sample. The methods may be performed by a near-field Fourier Ptychographic system.

A dark field illuminator for microscopic imaging is provided. The dark field illuminator is arranged above an adjustable lens group of a unit microscopic imaging module and corresponds to the adjustable lens group, a surface of the dark field illuminator is attached to a back of a sample slide, and the sample slide is located between the dark field illuminator and the adjustable lens group; the dark field illuminator includes a bright and dark field substrate and a dark field black background patch, the size of the dark field black background patch matches with that of the adjustable lens group, and the dark field black background patch is arranged close to or away from the adjustable lens group relatively to the bright and dark field substrate. Preferably, the bright and dark field substrate further has a recessed structure with a white diffuse reflection surface.

Apparatuses, systems and methods for generating color video with a monochrome sensor include the acts of (i) selectively energizing each of a plurality of light sources in a sequence, (ii) capturing a monochrome image of the illuminated sample at a monochrome sensor at each stage of the sequence, and (iii) generating a color video from the monochrome images. The sequence can have a series of stages with each stage of the sequence corresponding to activation of a different wavelength of light from the light sources to illuminate a sample. Generating the monochrome video can include the acts of compiling a plurality of monochrome images captured at the monochrome sensor with a single light source into a series of monochrome video frames comprising the monochrome video.

A ptychography system is presented for imaging an object located in an object plane. The ptychography system comprises an optical system, and a detection device. The optical system comprises a single shot ptychography arrangement configured and operable to create light response patterns from the object in the object plane on a pixel matrix of the detection device during the same exposure session of the detection device, wherein the optical system further comprises at least one light coding device configured and operable to apply at least one predetermined coding function to at least one of illuminating light and the light response of the object being collected, and said detection device is configured and operable with a predetermined duration of the exposure session during which the pixel matrix detects the collected light, such that image data indicative of the detected light during a single exposure session is in the form of a coded light response of the object being illuminated.