Described herein are improvements in digital microscopy and telepathology. The disclosed technologies enable users to configure digital microscopes remotely.

Disclosed herein are prosthesis simulator devices comprising a first restraint configured to restrain one or more fingers of a wearer of the simulator, a second restraint configured to restrain a thumb of the wearer, and a plurality of artificial digits configured to move in a manner to simulate one or more prosthetic fingers and a prosthetic thumb of a prosthesis. The first restraint can be attached to a roof plate connected to a base plate and defining a dorsal side of the prosthesis simulator. The second restraint can be attached to a holster connected to the base plate on a palmar side of the prosthesis simulator. Also disclosed herein are methods of using the same.

A multi-mode illumination system, including: a first illumination module; a second illumination module; and a third illumination module, as disclosed herein.

Apparatuses, systems and methods for generating color video with a monochrome sensor include the acts of (i) selectively energizing each of a plurality of light sources in a sequence, (ii) capturing a monochrome image of the illuminated sample at a monochrome sensor at each stage of the sequence, and (iii) generating a color video from the monochrome images. The sequence can have a series of stages with each stage of the sequence corresponding to activation of a different wavelength of light from the light sources to illuminate a sample. Generating the monochrome video can include the acts of compiling a plurality of monochrome images captured at the monochrome sensor with a single light source into a series of monochrome video frames comprising the monochrome video.

An observation device includes: an illumination optical system configured to emit illumination light to above a sample from below; and an objective optical system configured to acquire an image of transmitted light below the sample in a path different from a path for the illumination optical system, the transmitted light resulting from the illumination light that has been emitted from the illumination optical system, that has been reflected above the sample, and that has passed through the sample. The objective optical system includes, in the vicinity of a pupil plane, a pupil modulation element that partially decreases transmittance and that modulates the phase of light, and the illumination optical system includes a light source, and an illumination-region restricting section configured to restrict light from the light source to a particular emission region.

Described herein are systems and methods for assessing a biological sample. The methods include: characterizing a speckled pattern to be applied by a diffuser; positioning a biological sample relative to at least one coherent light source such that at least one coherent light source illuminates the biological sample; diffusing light produced by the at least one coherent light source; capturing a plurality of illuminated images with the embedded speckle pattern of the biological sample based on the diffused light; iteratively reconstructing the plurality of speckled illuminated images of the biological sample to recover an image stack of reconstructed images; stitching together each image in the image stack to create a whole slide image, wherein each image of the image stack at least partially overlaps with a neighboring image; and identifying one or more features of the biological sample. The methods may be performed by a near-field Fourier Ptychographic system.

The optical system for a microscope includes an illumination lens group G11 and an objective optical system. The objective optical system has an objective lens group G12, a first image forming lens group G13, and a second image forming lens group G14, which are disposed in this order from the object side, and satisfies the following conditional expressions. NA.sub.L×2≤NA.sub.O≤NA.sub.L×15 . . . (1); 0.01≤NA.sub.L≤0.1 . . . (2); and 25≤M.sub.O≤100 . . . (3), where NA.sub.L denotes the numerical aperture of the illumination lens group G11, NA.sub.O denotes the numerical aperture of the objective lens group G12, and M.sub.O denotes the image-formation magnification of the objective lens group G12.

A microscope lens system includes a body having a surface, a microlens, and an aperture positioned between the microlens and the surface. In the embodiment, the body is configured to position a mobile device on the surface such that a camera lens of the mobile device is aligned with the aperture.

A microscope and a microscopy method for imaging an object, wherein use is made of a microscope comprising an objective which defines an optical axis and a focal plane perpendicular thereto, and an adjustable correction optical unit which, at the objective, corrects a spherical aberration occurring when imaging the object with a certain depth position of the focal plane. The method comprises the following steps: determining an actual type of object; reading a database in which refractive indices of different types of objects are stored in order to determine the refractive index of the object; using a relationship between the refractive index and the spherical aberration caused by the object in order to ascertain an adjustment value of the correction optical unit such that there is a reduction in the spherical aberration in the focal plane; adjusting the correction optical unit to the adjustment value; and imaging the object.

A microscope and a microscopy method for imaging an object, wherein use is made of a microscope comprising an objective which defines an optical axis and a focal plane perpendicular thereto, and an adjustable correction optical unit which, at the objective, corrects a spherical aberration occurring when imaging the object with a certain depth position of the focal plane. The method comprises the following steps: determining an actual type of object; reading a database in which refractive indices of different types of objects are stored in order to determine the refractive index of the object; using a relationship between the refractive index and the spherical aberration caused by the object in order to ascertain an adjustment value of the correction optical unit such that there is a reduction in the spherical aberration in the focal plane; adjusting the correction optical unit to the adjustment value; and imaging the object.