An imaging system for a biological sample includes a sample container having at least one biological cell that is in contact with an interface surface of a container interface. The imaging system also includes illuminating optics that output a light beam aligned with a sample plane, the light beam being oriented horizontally along a transverse (XY) plane and illuminating the biological cell vertically along an axial (XZ) plane. The imaging system further includes imaging optics aligned horizontally along the transverse (XY) plane with the interface in the sample container, the imaging optics being configured to detect along the axial (XZ) plane a magnified image of a measurable contact angle between the biological cell and the interface surface. The measurable contact angle changes over time and is indicative of biological adhesion between the biological cell and another biological cell.

A microscope is a microscope that switches an observation method between the bright-field observation and the fluorescence observation. The microscope includes an objective that irradiates a sample with excitation light and converts fluorescence from the sample into a parallel light flux, a beam splitter that splits fluorescence and excitation light from each other, and a collective lens that is arranged in such a manner that it is freely set in and removed from an optical path between the beam splitter and the objective, that has a positive power, and that is set in the optical path for fluorescence observation and is removed from the optical path for bright-field observation.

A microscope is a microscope that switches an observation method between the bright-field observation and the fluorescence observation. The microscope includes an objective that irradiates a sample with excitation light and converts fluorescence from the sample into a parallel light flux, a beam splitter that splits fluorescence and excitation light from each other, and a collective lens that is arranged in such a manner that it is freely set in and removed from an optical path between the beam splitter and the objective, that has a positive power, and that is set in the optical path for fluorescence observation and is removed from the optical path for bright-field observation.

To provide a microscopic imaging device in which a measuring object can be easily imaged using measurement light having a desired pattern, in which the pattern of measurement light can be changed and a phase of the pattern can be moved, without arranging a mechanical mechanism. An arbitrary pattern of a plurality of patterns of measurement light is instructed. The measurement light having an instructed pattern is generated by a light modulation element, and is applied on a measuring object. A spatial phase of the generated pattern is sequentially moved on the measuring object by a predetermined amount by the light modulation element. A plurality of pieces of pattern image data generated at a plurality of phases of the pattern is synthesized based on the light receiving signal output from the light receiving section to generate sectioning image data indicating an image of the measuring object.

To provide a microscopic imaging device in which a measuring object can be easily imaged using measurement light having a desired pattern, in which the pattern of measurement light can be changed and a phase of the pattern can be moved, without arranging a mechanical mechanism. An arbitrary pattern of a plurality of patterns of measurement light is instructed. The measurement light having an instructed pattern is generated by a light modulation element, and is applied on a measuring object. A spatial phase of the generated pattern is sequentially moved on the measuring object by a predetermined amount by the light modulation element. A plurality of pieces of pattern image data generated at a plurality of phases of the pattern is synthesized based on the light receiving signal output from the light receiving section to generate sectioning image data indicating an image of the measuring object.

A high content imaging system and a method of operating the high content imaging system are disclosed. A microscope has a first objective lens and a second objective lens, and an objective lens database has first and second transformation values associated with the first and the second objective lenses, respectively. A microscope controller operates the microscope with the first objective lens to develop first values of acquisition parameters. A configuration module automatically determines second values of the acquisition parameters using the first values of the acquisition parameters, first transformation values associated with the first objective lens, and second transformation values associated with the second objective lens. The microscope controller operates the microscope using the second objective lens and the second values of the acquisition parameters.

An apparatus for enhancing an input phase distribution (I(x.sub.i)) is configured to retrieve the input phase distribution (I(x.sub.i)) and compute a baseline estimate (ƒ(x.sub.i)) as an estimate of a baseline (I.sub.2 (x.sub.i)) in the input phase distribution (I(x.sub.i)). The apparatus is further configured to obtain an output phase distribution (O(x.sub.i)) based on the baseline estimate (ƒ(x.sub.i)) and the input phase distribution (I(x.sub.i)).

An apparatus for enhancing an input phase distribution (I(x.sub.i)) is configured to retrieve the input phase distribution (I(x.sub.i)) and compute a baseline estimate (ƒ(x.sub.i)) as an estimate of a baseline (I.sub.2 (x.sub.i)) in the input phase distribution (I(x.sub.i)). The apparatus is further configured to obtain an output phase distribution (O(x.sub.i)) based on the baseline estimate (ƒ(x.sub.i)) and the input phase distribution (I(x.sub.i)).

A microscope comprising an illumination assembly, an image capture device and a processor can be configured to selectively identify regions of a sample comprising artifacts or empty space. By selectively identifying regions of the sample that have artifacts or empty space, the amount of time to generate an image of the sample and resources used to generate the image can be decreased substantially while providing high resolution for appropriate regions of the computational image. The processor can be configured to change the imaging process in response to regions of the sample that comprises artifacts or empty space. The imaging process may comprise a higher resolution process to output higher resolution portions of the computational image for sample regions comprising valid sample material, and a lower resolution process to output lower resolution portions of the computational image for sample regions comprising valid sample material.

The present invention relates to digital pathology. In order provide enhanced use of available imaging radiation, a digital pathology scanner (10) is provided that comprises a radiation arrangement (12), a sample receiving device (14), an optics arrangement (16), and a sensor unit (18). The radiation arrangement comprises a source (20) that provides electromagnetic radiation (22) for radiating a sample received by the sample receiving device. Further, the optics arrangement comprises at least one of the group of a lens (24) and a filter (26) that are arranged between the sample receiving device and the sensor unit. The sensor unit is configured to provide image data of the radiated sample. Still further, a lens array arrangement (28) is provided that comprises at least one lens array (30) arranged between the source and the sample receiving device. The at least one lens array comprises a plurality of linear cylindrical lenses (32) that modulate the electromagnetic radiation from the source such that, in an object plane, a radiation distribution pattern (34) is generated with a plurality of first parts of intensified radiation and a plurality of second parts of weak radiation.