System and method using a projected reference to guide adjustment of a correction optic. In an exemplary method, a reference may be projected onto an imaging detector by propagation of light generally along an optical axis that extends from the reference, through an objective, to a surface of a sample holder, and from the surface, back through the objective, to the imaging detector. The light may propagate through an off-axis aperture located upstream of the imaging detector and spaced from the optical axis. A plurality of images of the reference may be captured using the imaging detector, and with a correction optic at two or more different settings. A setting for the correction optic may be selected based on the plurality of images, and a sample may be imaged while the correction optic has the selected setting.

This disclosure discloses a microscope system, a smart medical device, an automatic focusing method, and a storage medium. The smart medical device includes an objective lens, a beam splitter, an image projector assembly, a camera assembly, and a focusing device. The objective lens includes a first end and a second end, and the first end faces a to-be-observed sample. The beam splitter is disposed on the second end. The image projector assembly is in communication with the beam splitter, the image projector assembly includes a first lens and an image projection device, and light generated by the image projector assembly enters the beam splitter through the first lens. The camera assembly includes a camera. The focusing device is disposed on the camera assembly, and the focusing device is configured to perform focus adjustment on the camera.

The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.

The present disclosure includes systems and methods for capture a whole slide image of a sample. In exemplary embodiments, a camera is configured to capture a digital image of a sample. The system captures a bright field image of the sample, and captures a digital image of the sample illuminated from a first incident angle at a first wavelength and a second incident angle at a second wavelength. The system can determine whether the sample is defocused based on the transitional shift between a first wavelength channel and a second wavelength channel of the captured digital image. The system can determine the defocus distance based on the transitional shift and autofocus using the defocus distance such the bright field image is in focus.

The present disclosure provides method and system for auto focusing a microscopic imaging system using machine learned regression system such as Convolutional Neural Network (CNN). The method comprises receiving a first image of a sample under review and a second image of sample wherein the first image is captured at first focus position and second image is captured at second focus position. The CNN is trained using the plurality of historic difference images along with direction of focus and optimal focus position. The difference of two images are obtained in terms of difference in pixel values. The direction of focus and optimal focus position for difference image is identified based on plurality of historic difference images along with direction of focus and optimal focus position. The method enables automated stage comprising sample to move towards direction of focus and position at optimal focus position for capturing a focused image.

Real-time focusing in a slide-scanning system. In an embodiment, focus points are added to an initialized focus map while acquiring a plurality of image stripes of a sample on a glass slide. For each image stripe, a plurality of frames, collectively representing the image stripe, may be acquired using both an imaging line-scan camera and a tilted focusing line-scan camera. Focus points, representing positions of best focus for trusted frames, are added to the focus map. Outlying focus points are removed from the focus map. In some cases, one or more image stripes may be reacquired. Finally, the image stripes are assembled into a composite image of the sample.

A medical observation system 1 is provided with an imaging unit 21 which captures an image of a subject to generate a captured image, a distance information acquiring unit which acquires subject distance information regarding subject distances from a specific position to corresponding positions on the subject that correspond to at least two pixel positions in the captured image, and an operation control section 264c which controls at least any of the focal position of the imaging unit 21, the brightness of the captured image, and the depth of field of the imaging unit 21 on the basis of the subject distance information.

An irradiation unit (14) projects excitation light (LB) having an asymmetric shape with respect to an optical axis (A1, A2). An objective lens (20) concentrates the excitation light (LB) at a measurement-target member (22) including a glass member (22C, 22A) and a measurement-target region (22B). The detection unit (30) includes at least one or more light-receiving units (31) that receive fluorescence emitted from the measurement-target region (22B) in response to the excitation light (LB), and outputs a fluorescence signal indicating intensity values of fluorescence received by the respective light-receiving units (31). The movement control unit (12C) includes a derivation unit (12B) that derives a movement amount and a movement direction of at least one of the objective lens (20) or the measurement-target member (22) on the basis of the fluorescence signal, and moves at least one of the objective lens (20) or the measurement-target member (22) by the derived movement amount in the derived movement direction.

A method is provided for operating a microscope scanner. A first imaging scan is performed of one or more area(s) of interest, AOI, on a target including a sample. This involves moving a detector array relative to the target along an image scan path and acquiring an image of the target at each of a plurality of locations along the image scan path. Focus control data is generated during the imaging scan by calculating a focus merit value at each said location along the image scan path. The focal height of the detector array is then adjusted along the image scan path based on the focus merit values. The efficacy of the first imaging scan is analysed using the focus control data and a change to one or more scanning parameters from the first imaging scan is determined, for the performance of a second imaging scan, based on this analysis.

This information processing apparatus includes: a search image acquisition unit that acquires enlarged images at focal positions different from each other; a first feature amount calculation unit that obtains, for each of the multiple captured enlarged images, a first feature amount based on a direct current component and a dynamic range of an alternating current component of pixel values for each of blocks forming each of the enlarged images; and an in-focus position determination unit that determines an in-focus position of each enlarged image based on the first feature amount.