Methods and systems are provided for synchronizing image capture at a multi-detector imaging system. In one example, a method includes coordinating cycling of each microscope assembly of the multi-detector imaging system through a selection of illumination channels, each microscope assembly configured to obtain an image of a portion of one of more than one microplate wells simultaneously, to generate complete images of the more than one microplate wells concurrently.

A high content imaging system and a method of operating the high content imaging system are disclosed. A microscope has a first objective lens and a second objective lens, and an objective lens database has first and second transformation values associated with the first and the second objective lenses, respectively. A microscope controller operates the microscope with the first objective lens to develop first values of acquisition parameters. A configuration module automatically determines second values of the acquisition parameters using the first values of the acquisition parameters, first transformation values associated with the first objective lens, and second transformation values associated with the second objective lens. The microscope controller operates the microscope using the second objective lens and the second values of the acquisition parameters.

A microscope system comprises: a light source; an objective lens; a stage; a two-dimensional image sensor that captures an image of a specimen placed on the stage; a focusing device that changes distance between the objective lens and the stage; and a control circuit, wherein the control circuit executes, during a movement period in which the stage moves in a direction orthogonal to an optical axis of the objective lens, focus control for controlling the focusing device based on focus evaluation information detected during the movement period, and exposure control for controlling an exposure period of the two-dimensional image sensor, and executes light emission control that causes the light source to emit light with different light emission intensities during the exposure period and during a focus evaluation period in which the focus evaluation information is detected.

The invention relates to an autofocusable microscope objective having an optical system of a plurality of optical components each formed by a lens or lens group. Here, one of the plurality of optical components is a liquid lens to effect autofocusing of the microscope objective, and the optical system is formed as a stationary system.

A light sheet microscope includes a sample chamber in which a cover slip or slide is arrangeable, which has a surface that defines a partially reflective interface and which has a further surface that defines a further partially reflective interface. The two interfaces are arranged at different distances from an objective. The light sheet microscope further includes an optical system having the objective facing toward the cover slip or slide, an illumination apparatus, which is designed to generate a light sheet, a sensor, and a processor. The two interfaces are formed in that two optical media are applicable in the sample chamber. The light sheet microscope forms a measuring device for acquiring a measured variable. The sensor is designed to acquire the intensities and/or the incidence locations of the two reflection light beams.

A digital microscope and a focusing thereof are provided. The microscope integrates a focusing lens unit, a zooming lens unit and related control circuits into the interior of the microscope. The control unit executes a focus searching algorithm and focus tracking algorithm, controls movement of the focusing lens unit and the zooming lens unit and achieves auto-focus by adjusting the image distance and makes the microscope zoom by adjusting the focal length. This improves the focusing efficiency and shortens the focusing time greatly. The microscope can achieve continuous zooming and tracking by the zoom tracking algorithm and the image is kept clear in the zooming process. The microscope adjusts the brightness of the assist light source at the front end of the microscope according to the change of the amount of light transmission by the control unit executing the exposure algorithm to ensure uniform exposure.

An imaging system includes: an image forming device that obtains image information of an object with the focal length to the object being changed by a variable focal length lens in an optical system, so as to form an all-focused image of the object; a sensor device that detects variations in the surface level of the object in a focus direction; and a position adjustment device that adjusts the position of the object in the focus direction in accordance with the surface level variations of the object detected by the sensor device. The position adjustment device adjusts the position of the object in the focus direction in such a manner that the surface level of the object becomes closer to the center of a variable focal length range of the variable focal length lens.

Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.

A method is used for optical scanning of at least one object region placed on a transparent specimen holder. The method is as follows: for each sample lateral position of plural predefined sample lateral positions performing a focus determination by: performing laser reflection and using a first camera taking plural first images to determine a reference distance between the specimen holder and an objective lens; performing transmission flash illumination and using a second camera taking plural second images to define a focus distance taking into account the reference distance; after completing the focus determination, determining a focus distance topology across the object region based on the focus distances determined for ail sample lateral positions; and laterally moving the specimen holder and acquiring third images while focusing according to the focus distance topology.

There are provided a microscope apparatus, an observation method, and a microscope apparatus-control program that can more efficiently perform auto-focus control and can shorten an imaging time in a case where a culture vessel is to be scanned by an image forming optical system and the auto-focus control is to be performed at each observation position. Focus information of a culture vessel is detected by a first displacement sensor and a second displacement sensor while a stage is moved to a scanning measurement position from an initial set position, and an auto-focus control unit performs auto-focus control at every observation position on the basis of the focus information in a case where the stage has been moved to the scanning measurement position.