The present disclosure disclose a microscopic device and a focusing method of the microscopic device, the microscopic device includes an object stage configured to carry a transparent carrier and drive the transparent carrier to translate along at least one direction, the transparent carrier includes at least two object positions; a microscopic objective located on a side of the object stage; a rangefinder located on a same side of the object stage as a microscopic objective and at a same height relative to the object stage, and the rangefinder is configured to measure a distance between a surface of each of the at least two object positions and the microscopic objective; a focusing module configured to adjust a position of the microscopic objective along a first direction based on the distance measured by the rangefinder, wherein the first direction is perpendicular to a carrying surface of the object stage.

A method comprises: directing, using an objective and a first reflective surface, first autofocus light toward a sensor, the first autofocus light reflected from a first surface of a substrate; preventing second autofocus light from reaching the sensor, the second autofocus light reflected from a second surface of the substrate; and directing, using the objective and a second reflective surface, emission light toward the sensor, the emission light originating from a sample at the substrate.

A method for laser distance measuring includes: emitting an incident ray α having an incident angle δ to a reflective surface of a laser reflecting mirror (31), the incident ray α being reflected by the laser reflecting mirror (31) to generate a first reflected ray β, and the first reflected ray β irradiating an object to be measured (1000); capturing a second reflected ray θ and generating a laser image on a laser imaging plane (3221), the second reflected ray θ being generated by the reflective surface of the laser reflecting mirror (31) reflecting a return ray γ generated after the first reflected ray β irradiates a surface of the object to be measured; and determining, based on the laser image, a measurement distance A according to a geometrical trigonometry.

Methods and systems are provided for microscope auto-focusing. In one example, a a method for microscope auto-focusing comprises: acquiring a plurality of contrast samples of an imaged subject; modeling a contrast distribution based on the plurality of contrast samples; acquiring an additional contrast sample based on the contrast distribution; modeling a corrected contrast distribution based on the additional contrast sample and the plurality of contrast samples; and focusing the imaged subject based on the corrected contrast distribution.

A method of using an imaging system including a focal plane with one or more detectors, a lens optically coupled to the focal plane, a transparent plate optically coupled to the focal plane and lens, and an actuator coupled to the transparent plate, includes receiving, at a first area of the focal plane through the lens, light from an object at a first time. The imaging system is located in a first position relative to the object at the first time. The method also includes causing the actuator to move the transparent plate in response to movement of the imaging system relative to the object and receiving, at the first area of the focal plane through the lens, light from the object at a second time. The imaging system is located in a second position relative to the object at the second time.

System for acquiring a digital image of a sample on a microscope slide. In an embodiment, the system comprises a stage configured to support a sample, an objective lens having a single optical axis that is orthogonal to the stage, an imaging sensor, and a focusing sensor. The system further comprises at least one beam splitter optically coupled to the objective lens and configured to receive a field of view corresponding to the optical axis of the objective lens, and simultaneously provide at least a first portion of the field of view to the imaging sensor and at least a second portion of the field of view to the focusing sensor. The focusing sensor may simultaneously acquire image(s) at a plurality of different focal distances and/or simultaneously acquire a pair of mirrored images, each comprising pixels acquired at a plurality of different focal distances.

A system 100 and method are provided for imaging a sample in a sample holder. For providing autofocus, a 2D pattern is projected onto the sample holder 050 via an astigmatic optical element 120. Image data 172 of the sample is acquired by an image sensor 140 via magnification optics 150. A difference in sharpness of the two-dimensional pattern in the image data is measured along a first axis and a second axis. Based on the difference, a magnitude and direction of defocus of the camera subsystem is determined with respect to the sample holder. This enables the sample holder, and thereby the sample, to be brought into focus in a fast and reliable manner.

The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.

A digital slide scanning apparatus that includes a scanning stage, a focusing sensor, an imaging sensor, and at least two processors. A main processor is configured to control the scanning stage to move a sample relative to the focusing sensor and the imaging sensor. The main processor controls the secondary processor to process focus buffers generated by the focusing sensor and image buffers generated by the imaging sensor. The secondary processor access each buffer and processes the data in the buffer to generate an average contrast vector for the buffer. The average contrast vectors for the focus and image buffers are then provided to the main processor for further processing in connection with autofocus and/or generation of a digital slide image.

A magnified observation apparatus includes an FOV changer, an in-focus degree evaluator, and a focus sequence executor. The in-focus degree evaluator calculates in-focus degree feature quantities, which represent in-focus degrees of image data corresponding to images to be displayed by a display controller on a display. The focus sequence executor executes an FOV-moving focus sequence in which a focusing device adjusts a relative distance between a focus position of the objective lens and an observation object in accordance with in-focus degree feature quantities of image data that are successively calculated by the in-focus degree evaluator corresponding to images of the observation object that are captured during movement of an observation FOV by the FOV changer so that a live image of the observation object is displayed on the display based on image data that is obtained during the movement of an observation FOV by the display controller.