Techniques are described for dynamically correcting image distortion during imaging of a patterned sample having repeating spots. Different sets of image distortion correction coefficients may be calculated for different regions of a sample during a first imaging cycle of a multicycle imaging run and subsequently applied in real time to image data generated during subsequent cycles. In one implementation, image distortion correction coefficients may be calculated for an image of a patterned sample having repeated spots by: estimating an affine transform of the image; sharpening the image; and iteratively searching for an optimal set of distortion correction coefficients for the sharpened image, where iteratively searching for the optimal set of distortion correction coefficients for the sharpened image includes calculating a mean chastity for spot locations in the image, and where the estimated affine transform is applied during each iteration of the search.

An acquisition condition is decided for a second image of improved quality. Values x.sub.i resulting from down sampling brightness values of an input first image are accepted by an input layer. A filter is scanned and a convolutional computation performed in a convolutional layer. Outputs z.sub.1 to z.sub.4 of the convolutional layer and a first image acquisition condition v=(v.sub.1, v.sub.2, v.sub.3, v.sub.4) of the first image are accepted by an output layer and a second acquisition condition y is computed by the output layer.

An intraoral scanner comprises a light source, a moveable opto-mechanical module, an axial actuator, and an image sensor. The light source is configured to generate light that is to be output onto an object external to the intraoral scanner. The moveable opto-mechanical module comprises integrated projection/imaging optics and an exit pupil, the projection/imaging optics having an optical axis, wherein the projection/imaging optics are entirely integrated into the moveable opto-mechanical module. The axial actuator is coupled to the projection/imaging optics and configured to move the moveable opto-mechanical module comprising an entirety of the projection/imaging optics in the optical axis to achieve a plurality of focus settings. The image sensor is configured to receive reflected light that has been reflected off of the object external to the intraoral scanner for the plurality of focus settings.

Apparatus and methods are described for use with a blood sample. Using a microscope (24), three images of a microscopic imaging field of the blood sample are acquired, each of the images being acquired using respective, different imaging conditions, and the first one of the three images being acquired under violet-light brightfield imaging. Using at least one computer processor (28), an artificial color microscopic image of the microscopic imaging field is generated, by mapping the first one of the three images to a red channel of the artificial color microscopic image, mapping a second one of the three images to a second color channel of the artificial color microscopic image, and mapping a third one of the three images to a third color channel of the artificial color microscopic image. Other applications are also described.

Systems and methods for passive multi-directional illumination in light-sheet fluorescence imaging and microscopy are disclosed herein. An elliptical light shaping diffuser is placed in the illumination path between the source of a light-sheet and the illuminated sample. The light-sheet is diffused anisotropically along two directions perpendicular to its propagation direction, eliminating stripe artifacts in obtained images. The method includes converting a light-sheet into an elliptically diffuse light-sheet by passing it through an elliptical light shaping diffuser, illuminating a sample with the elliptically diffuse light-sheet. The system includes a light-sheet source, an elliptical light shaping diffuser adapted to convert the light-sheet into an elliptically diffuse light-sheet to illuminate the sample, typical microscopy optics and lenses, and image capturing elements.

A process is provided for imaging a surface of a specimen with an imaging system that employs a BD objective having a darkfield channel and a bright field channel, the BD objective having a circumference. The specimen is obliquely illuminated through the darkfield channel with a first arced illuminating light that obliquely illuminates the specimen through a first arc of the circumference. The first arced illuminating light reflecting off of the surface of the specimen is recorded as a first image of the specimen from the first arced illuminating light reflecting off the surface of the specimen, and a processor generates a 3D topography of the specimen by processing the first image through a topographical imaging technique. Imaging apparatus is also provided as are further process steps for other embodiments.

A method for adjusting a focus of an optical system includes focusing measurement light in a sample space using an optical arrangement. The measurement light is transmitted on a sample side of the optical arrangement through at least one optical medium. The measurement light reflected by a reflector and transmitted through a further optical arrangement is detected using a detector arrangement. A working distance between the optical arrangement and the reflector is ascertained based on the measurement light detected by the detector, wherein a focus of the measurement light lies on the reflector for the working distance.

Systems and methods herein provide improved, high-throughput multiphoton imaging of thick samples with reduced emission scattering. The systems and methods use structured illumination to modify the excitation light. A reconstruction process can be applied to the resulting images to recover image information free of scattering. The disclosed systems and methods provide high throughput, high signal-to-noise ratio, and high resolution images that are depth selective.

A microscopic transmitted light contrasting method includes illuminating a sample through asymmetrical first and second illumination pupils and imaging the sample through asymmetrical first and second detection pupil in order to generate, respectively, first and second partial images. The first illumination pupil and the first detection pupil, as well as the second illumination pupil and the second detection pupil, are arranged pivoted in relation to one another and partially overlapping in projection on a plane perpendicular to an optical axis in such a way that first and third regions of an angular space are in a bright field and second and fourth regions of the angular space are in a dark field, and the first and second partial images each have a bright and a dark field component. An image of the sample is generated from the first and second partial images.

A high-power-microscope-assisted identification method of maize haploid plants is provided, the method is implemented by a device including a high power microscope, a main frame disposed on an objective table of the high power microscope and a computer and includes four procedures of sample information input, automatic testing of a batch of samples, automatic analysis and comparison, and automatic generation of data results. Vertical sliding grooves are symmetrically formed in the main frame, and a vertical supporting plate is disposed at an upper end of the main frame. Horizontal sliding grooves are symmetrically formed in the vertical supporting plate, and a horizontal supporting plate is disposed on the vertical supporting plate.