A cell visualization system includes a digital holographic microscopy (DHM) device, a training device, and a virtual staining device. The DHM device produces DHM images of cells and the virtual staining device colorizes the DHM images based on an algorithm generated by the training device using generative adversarial networks and unpaired training data. A computer-implemented method for producing a virtually stained DHM image includes acquiring an image conversion algorithm which was trained using the generative adversarial networks, receiving a DHM image with depictions of one or more cells and virtually staining the DHM image by processing the DHM image using the image conversion algorithm. The virtually stained DHM image includes digital colorization of the one or more cells to imitate the appearance of a corresponding actually stained cell.

Systems and methods for simultaneous multi-channel off-axis holography are described. Multi-channel imaging systems can include a light system including a plurality of light sources configured to generate illumination and reference beams at a plurality of wavelengths, an illumination system configured to illuminate a target object with the illumination beams, an optical assembly configured to receive a reflected target beam and condition the target beam for recording at an optical imaging system, and a reference system configured to propagate the reference beams to the optical imaging system. The reference beams are interfered with the target beam at the optical imaging system to create interference patterns, which can be recorded in a collective image having a plurality of side lobes. Holographic information in the side lobes can be combined to generate 3D images having a substantially reduced signal to noise ratio.

Refractive index of biological specimens is a source of intrinsic contrast that can be explored without any concerns of photobleaching or harmful effects caused by extra contrast agents. This feature also contains rich information that can be related to the metabolism of cells at the cellular and subcellular levels. The present invention relates to systems and methods that can provide, without any moving parts, the 3-D refractive index map of continuously flowing biological samples in a micro-fluidic channel, for example.

An image display system is provided. The image display system includes: at least one holographic image acquiring device each configured to obtain holographic image information of a scene; an image synthesis device configured to generate holographic image synthesis information based on at least a part of the holographic image information obtained by the at least one holographic image acquiring device; and an image reconstruction device configured to reconstruct a holographic synthetic image in accordance with the holographic image synthesis information. The image display system can synthesize holographic image information to combine several holographic three-dimensional display scenes into one holographic three-dimensional scene, so that the user can perceive display effects almost the same as the real scenes, improving user experience. An image display method is also provided.

Provided is a digital holographic imaging apparatus, comprising: an illumination portion (10) having an illumination light emission surface (32i) for emitting coherent light of a specific wavelength as illumination light toward an object (1) side relative to the illumination light emission surface (32i), and a reference light emission surface (32r) for emitting the coherent light, as reference light, in a direction opposite to the illumination light; and an image sensor (50) located on the reference light emission surface (32r) side of the illumination portion (10) and imaging an interference pattern between object light having been modulated by the object (1) and passed through the illumination portion (10) and the reference light of the illumination light, the image sensor (50) having a pixel array (51) comprising two-dimensionally aligned pixels.

An apparatus for viewing a biological sample that functions as both a microscope and an interferometer. A short-coherence light source directs light onto the sample. A Fourier transform lens and a pixel-array detector are positioned to collect light scattered by the sample. An optic fiber assembly conveys a reference beam from the short-coherence light source. The detector collects the reference beam and the signal beam and uses coherence gating to acquire interferometric image data. In some embodiments the axis of the incident light striking the sample and the axis of collected scattered signal light form an angle of less than 180 degrees and advantageously an angle between 120 and 150 degrees. A method of converting a microscope into an interferometer is also disclosed.

The present invention provides an ellipsometry device and an ellipsometry method whereby measurement efficiency can be enhanced. In this method, an object is illuminated by spherical-wave-like illumination light Q linearly polarized at 45 (S1), and an object light O, being a reflected light, is acquired in a hologram I.sub.OR using a spherical-wave-like reference light R having a condensing point near the condensing point of the illumination light Q, and a hologram I.sub.LR of the reference light R is furthermore acquired using a spherical-wave reference light L having the same condensing point as that of the illumination light Q (S2). The holograms are separated into p- and s-polarized light holograms I.sup.K.sub.OR, I.sup.K.sub.LR, =p, s and processed to extract object light waves, and object light spatial frequency spectra G.sup.K(u, v), =p, s are generated (S3) (S4). Ellipsometric angles (), () are obtained for each incident angle from the amplitude reflection coefficient ratio =G.sup.p/G.sup.s=tan .Math.exp(i). Through use of numerous lights having different incident angles included in the illumination light Q, data of numerous reflection lights can be acquired collectively in a hologram and can be processed.

The present invention relates to a high-speed imaging system for measuring a target object within a sample, comprising: a light source emitting a plane wave; an angle-adjustment mirror adjusting an angle of the plane wave emitted from the light source; an optical interferometer dividing the plane wave whose angle was adjusted by the angle-adjustment mirror into a reference wave and a sample wave and forming an interference wave between the reference wave reflected from a reference mirror and the sample wave reflected from the target object; a camera module obtaining the interference wave, and an imaging controller controlling the angle-adjustment mirror to adjust the angle of the plane wave sequentially, forming a time-gated reflection matrix by using the interference waves obtained by the camera module in accordance with each angle of the plane wave, and imaging the target object based on the time-gated reflection matrix.

A method of structured illumination digital holography includes: (a) providing a structured illumination generating unit and binarization random number encoding unit to generate a coded structured illumination pattern; (b) sampling at least two patterns with phase shift which synthesized as a single structured illumination pattern to be encoded; (c) forming a single digital hologram, and wavefront reconstructing the single digital hologram; (d) performing a compressive sensing approach to recover the object wave with at least two phase shift patterns; and (e) reconstructing the separation of overlap spectrum, to obtain an image covering bandpass spectrum with different high frequency and low frequency.

Light reflected from an illuminated object is mixed with a reference beam and sensed at a sensor array of a digital hologram apparatus. Digital hologram data, determined from the sensed light, is dependent upon complex valued reflection coefficients of the object and upon phase perturbations in propagation paths between the object and the sensor array. Reflectance values, which may be dependent upon expected values of the absolute square of the reflection coefficients, and phase perturbations are determined for which a test function is at an extremum, where the test function contains a data fidelity term dependent upon the hologram data from a single hologram, a first regularization term dependent upon the phase perturbations and a second regularization term dependent upon the reflectance values. An image of the object may be formed from the reflectance values and a wavefront of the reflected light may be determined from the phase perturbations.