Apparatuses and methods for improved reconstructions of electron-based holograms are disclosed herein. An example method at least includes forming a hologram of a sample and a known object, forming a reconstruction of the known object using a reconstruction algorithm, comparing the reconstruction of the known object to a reference reconstruction of the known object, and adjusting the reconstruction algorithm based on the comparison of the reconstruction of the known object to the reference reconstruction of the known object. The example method may further include forming a reconstruction of the sample using the adjusted reconstruction algorithm.

Apparatuses and methods for comparative holographic imaging to improve structural and molecular information of reconstructions is disclosed herein. An example method at least includes acquiring a plurality of holograms of a sample, wherein each hologram of the plurality of holograms is acquired at a different electron beam energy, and determining atomic and structural information of the sample based at least on a comparison of at least two of the holograms of the plurality of holograms.

An in-line holographic microscope can be used to analyze a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. An assay using the holographic microscope for holographic particle characterization directly detect viruses, antibodies and related targets binding to the surfaces of specifically functionalized micrometer-scale colloidal probe beads. The system detects binding of targets by directly measuring associated changes in the bead's diameter without the need for downstream labeling and analysis.

The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.

A self-interference digital holographic system obtains interference patterns of incident light using a simple geometric phase lens, and obtains a holographic image of a target object using the interference patterns. The self-interference digital holographic is fabricated simply in a low cost and in a miniaturized size, and the use thereof as actual products is extended to a wide range of applications. The phase of incident light is be changed by rotating a polarizer, independently of a change in the optical path. Phase-shifting effects are obtained with fewer errors in all wavelength ranges, and a more accurate holographic image is produced. A single birefringence hologram is obtained by a one-time image-capturing process by simultaneously forming interference patterns from phase-shifted linearly-polarized beams by space division, using a phase shifter on the basis of space division. Moving holographic images can be captured.

A method and system for identification of holographic tracking and identification of features of an object. A holograph is created from scattering off the object, intensity gradients are established for a plurality of pixels in the holograms, the direction of the intensity gradient is determined and those directions analyzed to identify features of the object and enables tracking of the object. Machine learning devices can be trained to estimate particle properties from holographic information.

A device and method for forming an image of a sample includes illuminating the sample with a light source; acquiring a plurality of images of the sample using an image sensor, the sample being placed between the light source and the image sensor, no magnifying optics being placed between the sample and the image sensor, the image sensor lying in a detection plane, the image sensor being moved with respect to the sample between two respective acquisitions, such that each acquired image is respectively associated with a position of the image sensor in the detection plane, each position being different from the next; and forming an image, called the high-resolution image, from the images thus acquired.

A system and method for sorting and printing of cancer cells in general and more specifically a system and a method for enriching cancer cells in a sample using magneto-phoresis and cytology rotor in cell centrifuge using cytospin method. The present invention comprises a technological advantage over known systems and methods by use of a dynamic system wherein a fluid flow comprising cells flow across a magnetic field to separate cells based on absence or presence of paramagnetic nano particles attached to them.

A hologram image acquiring apparatus includes a linear polarizer that filters incident light reflected by an object into a polarized component of a specific angle; a spherical lens that partially converts light that is incident through the linear polarizer to a spherical waveform; and a phase shifter that converts a part of the light incident through the spherical lens to a plane waveform having a respective phase per pixel unit.