Systems and methods are provided for counting particles in a fluid flow. In an aspect, coordinates of particles are obtained from video data of particles in a fluid, the video data made up of a sequence of image frames. The particle positions are linked in each pair of consecutive image frames of the video data. The linked particle positions are used to calculate particle trajectories through sequential image frames of the video data, and the particles are counted based on the particle trajectory. In another aspect, the particle positions within each image frame are transformed to estimated positions within a common coordinate frame. The estimated particle positions of a particle are grouped into a cluster center, and the particle count is calculated based on the cluster centers.

A method for observing a sample (10), the sample lying in a plane of the sample defining radial coordinates, the method comprising the following steps: a) illuminating the sample using a light source (11), able to emit an incident light wave (12) that propagates toward the sample along a propagation axis (Z); b) acquiring, using an image sensor (16), an image (I.sub.0) of the sample (10), said image being formed in a detection plane (P.sub.0), the sample being placed between the light source (11) and the image sensor (16), such that the incident light wave sees an optical path difference, parallel to the propagation axis (Z), by passing through the sample; c) processing the image acquired by the image sensor;
wherein the processing of the acquired image comprises taking into account vectors of parameters, respectively defined at a plurality of radial coordinates, in the plane of the sample, each vector of parameters being associated with one radial coordinate, and comprising a term representative of an optical parameter of the sample, at least one optical parameter being an optical path difference induced by the sample at the radial coordinate, the vectors of parameters describing the sample.

The present invention includes method and device for label-free holographic screening and enumeration of tumor cells in blood for use in connection with cancer treatments and monitoring.

A method for determining a parameter of a particle present in a sample, the method comprising the following steps: a) illuminating the sample with the light source, the light source emitting an incident light wave that propagates along a propagation axis; b) acquiring an image of the sample with the image sensor, the image sensor being exposed to an exposure light wave; c) determining a position of the particle in the detection plane; d) on the basis of the acquired image, applying a propagation operator, for a plurality of distances from a detection plane, so as to estimate, at each distance, a complex amplitude of the exposure light wave; e) on the basis of the complex amplitude estimated, at various distances, obtaining a profile representing a variation of the complex amplitude of the exposure light wave along an axis parallel to the propagation axis and passing through the position of the particle.

The particle may associated with a set of parameters, comprising at least a size of the particle and a refractive index of the particle.

A method of structured illumination digital holography includes: (a) providing a structured illumination generating unit and binarization random number encoding unit to generate a coded structured illumination pattern; (b) sampling at least two patterns with phase shift which synthesized as a single structured illumination pattern to be encoded; (c) forming a single digital hologram, and wavefront reconstructing the single digital hologram; (d) performing a compressive sensing approach to recover the object wave with at least two phase shift patterns; and (e) reconstructing the separation of overlap spectrum, to obtain an image covering bandpass spectrum with different high frequency and low frequency.

A system for detecting objects in a specimen includes a chamber for holding at least a portion of the specimen. The system also includes a lens-free image sensor for obtaining a holographic image of the portion of the specimen in the chamber. The system further includes a processor in communication with the image sensor, the processor programmed to obtain a holographic image having one or more objects depicted therein. The processor is further programmed to obtain at least one object template representing the object to be detected, and to detect at least one object in the holographic image.

A method for observing a sample is placed between a light source and an image sensor, comprising at least 10000 pixels, the light source emits an illuminating beam, which propagates to the sample, the light beam is emitted in an illumination spectral band (.sub.11) lying above 800 nm, the method comprising the following steps: (a) illuminating the sample with the light source; (b) acquiring an image of the sample (I.sub.0) with the image sensor, no image-forming optics being placed between the sample and the image sensor; and (c) the image sensor being configured such that it has a detection spectral band (.sub.20), which blocks wavelengths in the visible spectral band, such that the image may be acquired in ambient light.

In a microscopic observation unit (10), hologram data is acquired at each measurement position on a cell culture plate (13) while a light-source section (11) and other elated sections are gradually moved by a moving section (15). Every time a set of data for one measurement position is acquired, a measurement monitoring image creator (4) creates a thumbnail age by reducing the size of a hologram image which is based on original data (two-dimensional distribution of light intensity). A display processor (25) pastes the create thumbnail image to progressively complete the hologram image of the entire plate to be displayed on a display unit (27). A measurement operator watches the hologram image during the execution of the measurement. When it has been concluded that the ongoing measurement is inappropriate, the operator presses a measurement stop button to immediately discontinue the measurement. Thus, When there is a problem with the measurement, such as a foreign object mixed in the sample, the measurement can be discontinued before a phase image or intensity image based on the hologram data is reconstructed on the server after the completion of the entire measurement, so as to avoid wasting time for the useless measurement.

A system for measuring vibrations of a surface (VSURF) of a mechanical part (M), comprising a source (SOURCE) of radiation (L), a first separator element (ELI) configured to define a first incident ray (LB1) and a reference ray (RLB), a shaping module (DOEM) producing a second incident ray (LB2) from said first incident ray (LB1), and an optical element (OE) capable of an interferential addition of the reference ray (RLB) and a ray produced by a reflection of said second incident ray (LB2) on said surface (VSURF), the shaping module (DOEM) comprising one or more diffracting optical elements (DOE1, . . . , DOEn), each comprising at least one diffraction structure (FSTRUCT) diffracting all or part of the first incident ray (LB1) so as to illuminate a chosen surface of the mechanical part.

A system or device includes a member structure, a plurality of flexible members, and a plurality of tips disposed at ends of the flexible members. The member structure includes an ultrasonic emitter configured to emit an ultrasonic imaging signal. The plurality of flexible members are coupled to the member structure. The plurality of tips are disposed at ends of the flexible members. At least one tip of the plurality of tips includes an image sensor configured to receive an infrared exit signal.