A method having the steps of obtaining prepared image data captured by an image sensor receiving light propagated across a sample volume, containing a fluid possibly comprising microscopic objects of foreign origin, while illuminating the sample volume by coherent light. The prepared image data comprising, for a microscopic object, a prepared hologram pattern with prepared spatially alternating intensity formed by the interference fringes; providing filtered image data, comprising automatically filtering the prepared image data by an edge enhancing filter. the filtered image data comprising, for a prepared hologram pattern, a filtered hologram pattern. The presence of the microscopic object associated with the filtered hologram pattern in the sample volume of the fluid is automatically detected on the basis of the filtered hologram pattern.

A method of training AI for label-free cell viability determination includes a step of providing a cell sample, a step of obtaining a fluorescence image and a DHM image of the cell sample, a step of determining a first cell viability of the cell sample according to the fluorescence image of the cell sample, a step of labeling the DHM image of the cell sample as a model specifying the first cell viability, and a step of performing AI training by using the model containing the DHM image of the cell sample.

The present invention relates to an inline scanning holography system for a phosphor and a transmitter. According to the present invention, the inline scanning holography system includes a polarization sensitive lens that receives a linearly polarized beam and generates a first spherical wave of right-handed circular polarized light having a negative focal length and a second spherical wave of left-handed circular polarized light having a positive focal length, a polarizer that passes only a beam component in a predetermined polarization direction therethrough among components of the generated first and second spherical waves, a scanning unit for scanning a phosphor by using an interference beam generated between the first and second spherical waves passing through the polarizer, and a first photodetector that detects a fluorescent beam diverged from the phosphor. According to the present invention, a high-efficiency and high-quality optical scanning holography for a phosphor or a transmitter may be implemented.

A holographic microscopy characterization (HMC) process for utilizing holographic video microscopy to provide an efficient, automated, label-free method of accurately identifying cell viability. Optical properties of a sample of cells are determined by HMC. The optical properties are compared to known samples or compared over time to observe changes in the optical properties, enabling identification of cells as viable or not viable, or as extra-cellular or degraded cellular materials.

A holographic imaging device and method realizes both a transmission type and a reflection type, and also realizes a long working distance wide field of view or ultra-high resolution. Object light emitted from an object, sequentially illuminated with parallel illumination light whose incident direction is changed, is recorded on a plurality of object light holograms for each incident direction using off-axis spherical wave reference light. The reference light is recorded on a reference light hologram using in-line spherical wave reference light being in-line with the object light. An object light wave hologram and its spatial frequency spectrum at the object position are generated for each incident direction using each hologram. A synthetic spectrum which occupies a wider frequency space is generated by matching each spectrum in the overlapping area, and a synthetic object light wave hologram with increased numerical aperture is obtained thereby.

The present disclosure concerns an electron microscopy method, including the emission of a precessing electron beam and the acquisition, at least partly simultaneous, of an electron diffraction pattern and of intensity values of X rays.

A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity term and backward gradient descent (110) on the regularization term.

A system for analyzing a transparent particle including: an analysis pathway, including a first light source emitting an analysis light beam, and a first optical system focusing the analysis light beam in a focusing plane; and a position control pathway including a second light source, an image sensor, and a second optical system at least partially merged with the first optical system. The image sensor is offset relative to the image of the focusing plane by the second optical system. The system makes it possible to control correct positioning of the particle, even though it is transparent, and without disturbing the analysis pathway.

The invention relates to a system (1) for observing objects, including: a light source (3), a holder (12) able to receive a translucent or opaque substrate, a detector (7) able to collect the backscattered light from the interaction between the light emitted by light source (3) and the objects, a polarization splitter (9), and a quarter-wave plate (10), the splitter (9) and the quarter-wave plate (10) being arranged so that the splitter (9) directs the light emitted by the light source (3) toward the solid substrate and directs the backscattered light from the interaction between the light emitted by the light source (3) and the objects toward the detector (7).

Disclosed is a method of measuring red blood cell membrane fluctuations based on dynamic cell parameters using a digital holographic microscope; the method including a step of modeling the three-dimensional images of red blood cells to be measured, and a step of measuring red blood cell membrane fluctuations based on the three-dimensional images. According to this method, since the three-dimensional images of red blood cells to be measured are modeled and red blood cell membrane fluctuations are measured based on the three-dimensional images, red blood cell membrane fluctuations can be measured more easily.