A method of imaging includes illuminating a sample spaced apart from an image sensor at a multiple distances. Image frames of the sample obtained at each distance are registered to one another and lost phase information from the registered higher resolution image frames is iteratively recovered. Amplitude and/or phase images of the sample are reconstructed based at least in part on the recovered lost phase information.

A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.

An apparatus for producing a hologram of an object includes a light source that emits an incoherent electromagnetic wave toward the object, and a masking device configured to display a mask, receive the incoherent electromagnetic wave emitted toward the object, mask the received incoherent electromagnetic wave according to the displayed mask, and produce a masked electromagnetic wave. The apparatus also includes an image recording device configured to capture an image of the masked electromagnetic wave, and a processing device configured to convert the image of the masked electromagnetic wave into the hologram of the object. A method for producing a hologram of an object is also described.

The present invention relates to a system and method for displaying and capturing holographic true 3D images. The system comprises elements which may form both a wide viewing angle holographic true 3D display and a holographic true 3D video camera. The system mainly comprises a light source, a spatial light modulator or an electro-optical capturing device in different embodiments of the invention, a phase plate, a computer and an opaque mask in some embodiments of the invention.

A method for obtaining a high resolution image of objects contained within a sample is disclosed that combines pixel super-resolution and phase retrieval techniques into a unified algorithmic framework that enables new holographic image reconstruction methods with significantly improved data efficiency, i.e., using much less number of raw measurements to obtain high-resolution and wide-field reconstructions of the sample. Using the unified algorithmic framework, twin image noise and spatial aliasing signals, along with other digital holographic artifacts, can be interpreted as noise terms modulated by digital phasors, which are all analytical functions of the imaging parameters including e.g., the lateral displacement between the hologram and the sensor array planes (x, y shifts), sample-to-image sensor distance (z), illumination wavelength (), and the angle of incidence (,).

An apparatus for producing a hologram includes a collimation lens configured to receive incoherent light emitted from an object; a spatial light modulator (SLM) that includes at least one diffractive lens which is configured to receive the incoherent light from the collimation lens and split the incoherent light into two beams that interfere with each other; and a camera configured to record the interference pattern of the two beams to create a hologram, wherein a ratio between a distance from the SLM to the camera and a focal length of the diffractive lens is greater than 1.

A system for three dimensional imaging of an object contained within a sample includes an image sensor, a sample holder configured to hold the sample, the sample holder disposed adjacent to the image sensor, and an illumination source comprising partially coherent light. The illumination source is configured to illuminate the sample through at least one of an aperture, fiber-optic cable, or optical waveguide interposed between the illumination source and the sample holder, wherein the illumination source is configured to illuminate the sample through a plurality of different angles.

The image observation apparatus introduces an object light, which is at least part of an object illumination light emitted from a light source and projected onto an object and which is reflected by the object, through a first optical waveguide to an image sensor, introduces a reference light, which is emitted from the light source and passes through an optical path different from that of the object light, to the image sensor, and records an interference fringe through the image sensor as a hologram. The apparatus forms the recorded hologram on a spatial light modulator and illuminate the modulator with a hologram illumination light corresponding to the reference light to generate a reconstruction light, and causes the reconstruction light entering a second optical waveguide optically equivalent to the first optical waveguide and exiting from the second optical waveguide to form an object reconstructed image.

An e-Petri dish comprising a transparent layer having a specimen surface and a light detector configured to sample a sequence of sub-pixel shifted projection images of a specimen located on the specimen surface. The sub-pixel shifted projection images associated with light from a plurality of illumination angles provided by an illumination source.

The present disclosure relates to devices and methods configured to perform drug screening on cells. At least one embodiment relates to a lens-free device for performing drug screening on cells. The lens-free device includes a substrate having a surface. The lens-free device also includes a light source positioned to illuminate the cells, when present, on the substrate surface with a light wave. The lens-free device further includes a sensor positioned to detect an optical signal caused by illuminating the cells. The substrate surface includes a microelectrode array for sensing an electrophysiological signal from the cells.