In one embodiment, a self-interference incoherent digital holography system including a light sensor and a diffractive filter configured to receive light from an object to be holographically imaged and generate holographic interference patterns on the light sensor. A self-interference incoherent digital holography system comprising: a light sensor; and a diffractive filter configured to receive light from an object to be holographically imaged and generate holographic interference patterns on the sensor.

A lens-free system for the three-dimensional imaging of objects contained within a sample places a sample holder between an image sensor and an illumination source, with the sample-sensor distance being much smaller than the sample-illumination source distance. Holographic images are taken at different angles as well as different lateral jogs within a single angle and are reconstructed into a three dimensional image of objects within the sample. The system may be a hand held, portable unit.

The invention describes a device allowing the observation of a sample, comprising particles, for example biological particles, by lensless imaging. The sample is disposed against a substrate, the substrate being interposed between a light source and an image sensor. The substrate comprises at least one thin film, extending across a thin film plane, structured so as to form a diffraction grating, designed to confine a part of a light wave emitted by the light source, in a plane parallel to said thin film plane. The device does not comprise magnification optics between the substrate and the image sensor.

The present invention is related to an holographic probe device for recording a gabor hologram comprising: a coherent optical fiber bundle comprising a distal end and a proximal end; a recording medium optically coupled to the proximal end of the coherent optical fiber bundle; a light source producing in use a single light beam, illuminating the distal end of the coherent optical fiber bundle and the object to be observed.

A method of generating a hologram of an object is disclosed. The method comprises: receiving data corresponding to a plurality of non-coherent sub-holograms acquired by an optically passive synthetic aperture holographic apparatus, combining the sub-holograms to generate a mosaic hologram of the object, and transmitting the mosaic hologram to a computer readable medium.

A system for three dimensional imaging of motile objects includes an image sensor and a sample holder disposed adjacent to the image sensor. A first illumination source is provided and has a first wavelength and positioned relative to the sample holder at a first location to illuminate the sample. A second illumination source is also provided having a second wavelength, different from the first wavelength, and positioned relative to the sample holder at a second location, different from the first location, to illuminate the sample. The first and second illumination sources are configured to simultaneously, or alternatively, sequentially illuminate the sample contained within the sample holder. Three dimensional positions of the motile objects in each frame are obtained based on digitally reconstructed projection images of the mobile objects obtained from the first and second illumination sources. This positional data is connected for each frame to obtain 3D trajectories of motile objects.

A device and method for sorting objects immersed in a flowing medium are disclosed. An example device comprises a holographic imaging unit comprising one or more holographic imaging elements, a fluid handling unit comprising one or more microfluidic channels configured to conduct flowing medium along a corresponding holographic imaging element and at least one microfluidic switch arranged downstream of an imaging region in the microfluidic channel configured to direct objects in the flowing medium into a one of a plurality of outlets. The example device also comprises a processor configured to determine real-time characterizations of holographic diffraction images obtained for the moving objects. The processing unit is further configured to control the at least one microfluidic switch in response to the real-time characterizations.

A method and system for identification of holographic tracking and identification of features of an object. A holograph is created from scattering off the object, intensity gradients are established for a plurality of pixels in the holograms, the direction of the intensity gradient is determined and those directions analyzed to identify features of the object and enables tracking of the object. Machine learning devices can be trained to estimate particle properties from holographic information.

The present method includes a data acquisition process and tomographic image generation processes. In the data acquisition process, holograms of an object light and so forth are acquired for each light with a wavelength by changing the wavelengths of the illumination light, off-axis spherical wave reference light, and inline spherical wave reference light. In the tomographic image generation process, a reconstructed light wave of the object light and a reconstructed light wave of the illumination light on a reconstruction surface are generated from these holograms. A reconstruction light wave with adjusted phase is added up for each wavelength to generate a tomographic hologram. From this, an accurate and focused tomographic image without distortion can be generated.

A method for reconstructing optical properties of a diffracting object immersed in a liquid medium using a reconstruction system that comprises a spatially coherent light source and a matrix photodetector, wherein the liquid medium and the matrix photodetector are separated by a distance along a vertical direction. The method comprises illuminating the liquid medium, measuring (with the matrix photodetector) an intensity of a diffraction pattern transmitted by the illuminated medium along a vertical direction, and reconstructing the optical properties of the diffracting object at a reconstruction height according to a reconstruction algorithm from the measured intensity, wherein the reconstruction height has a value less than that of the distance between the medium and the matrix photodetector along the vertical direction.