An apparatus and method to produce a hologram of an object includes an electromagnetic radiation assembly configured to receive a received electromagnetic radiation, such as light, from the object. The electromagnetic radiation assembly is further configured to diffract the received electromagnetic radiation and transmit a diffracted electromagnetic radiation. An image capture assembly is configured to capture an image of the diffracted electromagnetic radiation and produce the hologram of the object from the captured image.

The present disclosure presents systems, apparatuses, and methods of holographic imaging. In this regard, a method comprises transmitting light and illuminating a semi-transparent sample object; and forming, at a hologram plane, an interference pattern of a real image of the sample object from a scattered object beam and an unscattered reference beam from the transmitted light. To do so, the scattered object beam and the unscattered reference beam are in-line with one another, and a distance between the hologram plane to the sample object is set at a distance that substantially weakens a virtual image of the sample object formed from the scattered object beam and the unscattered reference beam. Accordingly, the method further comprises recording the interference pattern of a hologram formed from the scattered object beam and the unscattered reference beam at a detector; and reconstructing a 3D optical field of the hologram without phase retrieval.

An optical scanning holography system includes a polarization-sensitive lens configured to receive a linearly polarized beam and generate a first spherical wave of right-handed circular polarized light having a negative focal length and a second spherical wave of left-handed circular polarized light having a positive focal length, a first polarizer configured to pass only a beam component therethrough in a predetermined polarization direction among components of the generated first and second spherical waves, a scanning unit configured to scan an object by using an interference beam generated between the first and second spherical waves passing through the first polarizer, and a first photodetector configured to detect a beam reflected from the object.

Apparatuses and methods for improved reconstructions of electron-based holograms are disclosed herein. An example method at least includes forming a hologram of a sample and a known object, forming a reconstruction of the known object using a reconstruction algorithm, comparing the reconstruction of the known object to a reference reconstruction of the known object, and adjusting the reconstruction algorithm based on the comparison of the reconstruction of the known object to the reference reconstruction of the known object. The example method may further include forming a reconstruction of the sample using the adjusted reconstruction algorithm.

Example methods, apparatuses, and computer program products related to analyzing fluid samples are provided. For example, an example computer-implemented method for analyzing fluid samples includes receiving digital holography image data associated with a fluid sample in a flow chamber device; extracting, from the digital holography image data, an upper reference mark image region associated with an upper reference mark and a lower reference mark image region associated with a lower reference mark; determining a maximum focal depth and a minimum focal depth associated with the digital holography image data, respectively; focusing each of a plurality of focal depth layers associated with the digital holography image data; and extracting, from the plurality of focal depth layers, one or more region of interest (ROI) portions that are associated with the fluid sample.

An interference imaging device includes a light interference generator that includes: a light wave splitter configured to reflect a part of incident light and to allow a remaining part of the incident light to pass through; a phase modulator configured to modulate a phase of incident light that has passed through the light wave splitter; and a reflector configured to reflect the phase-modulated incident light from the phase modulator so that the reflected, phase-modulated incident light overlaps with incident light that has been reflected by the light wave splitter.

A light source device connected to an optical fiber and emit light from the optical fiber, the device includes: a plurality of laser light sources to respectively emit light at different wavelengths; a current source to supply a drive current with a superimposed alternating-current component to each laser light source; a light source control section to selectively switch the laser light sources by controlling the current sources; a plurality of optical systems disposed in optical paths of the respective laser light sources to reflect the light from the respective laser light sources to an incident end of the optical fiber and to reflect return light reflected on the incident end to the respective laser light sources; and a return light adjustment section to adjust an amount of the return light to continuously spread a spectrum of the light emitted from the optical fiber.

An optical scanning holography system includes a polarization-sensitive lens configured to receive a linearly polarized beam and generate a first spherical wave of right-handed circular polarized light having a negative focal length and a second spherical wave of left-handed circular polarized light having a positive focal length, a first polarizer configured to pass only a beam component therethrough in a predetermined polarization direction among components of the generated first and second spherical waves, a scanning unit configured to scan an object by using an interference beam generated between the first and second spherical waves passing through the first polarizer, and a first photodetector configured to detect a beam reflected from the object.

An optical scanning holography system includes a polarization-sensitive lens configured to receive a linearly polarized beam and generate a first spherical wave of right-handed circular polarized light having a negative focal length and a second spherical wave of left-handed circular polarized light having a positive focal length, a first polarizer configured to pass only a beam component therethrough in a predetermined polarization direction among components of the generated first and second spherical waves, a scanning unit configured to scan an object by using an interference beam generated between the first and second spherical waves passing through the first polarizer, and a first photodetector configured to detect a beam reflected from the object.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.