An imaging system includes a phase grating overlying a two-dimensional array of pixels, which may be thermally sensitive pixels for use in infrared imaging. The phase grating comprises a two-dimensional array of identical subgratings that define a system of Cartesian coordinates. The subgrating and pixel arrays are sized and oriented such that the pixels are evenly distributed with respect to the row and column intersections of the subgratings. The location of each pixel thus maps to a unique location beneath a virtual archetypical subgrating. Portions of the phase grating extend beyond the edges of the pixels array to interference pattern in support of Fourier-domain imaging.

A system for recording a digital hologram of an object comprises: a coherent source intended to illuminate the object and thus produce a wave diffracted by the object; and a digital sensor intended to record the digital hologram of the object. It furthermore comprises a spatial phase modulating assembly able to produce in the plane of the sensor a plurality of duplicates of the wave diffracted by the object, the duplicates being offset from each other but overlapping partially, these duplicates forming on the sensor a digital hologram of the object, this hologram being what is referred to as a self-reference hologram.

Among other things, a method comprises imaging a sample displaced between a sensor surface and a surface of a microscopy sample chamber to produce an image of at least a part of the sample. The image is produced using lensless optical microscopy, and the sample contains at least blood from a subject. The method also comprises automatically differentiating cells of different types in the image, generating a count of one or more cell types based on the automatic differentiation, and deriving a radiation dose the subject has absorbed based on the count.

A system for motility contrast imaging a biological target within tissue comprising a CCD array; an illumination source for generating an incoming beam; a first beam splitter for receiving the incoming beam and producing an object beam and a reference beam; a second beam splitter for illuminating a multitude of biological targets with the object beam and for directing backscattered object beams towards the CCD array; a computer-controlled delay stage for zero-path-matching the reference beam to the backscattered object beams; a reference beam that intersects the backscattered object beams at an angle to produce a series of interference fringes that modulate Fourier-domain information; and a computer for receiving a time series of Fourier-domain information. The interference fringes between the backscattered object beam and the reference beam are recorded by the CCD array and passed to the computer which constructs a digital hologram at successive times.

An apparatus for capturing a Fourier hologram splits a coherent light source into an object beam and a reference beam, sets a distance of a virtual focal plane of a subject, generates spherical wave field data propagated from a point source of the virtual focal plane to a central coordinate plane of a spatial light modulator, generates a spherical wave field on a space from the spherical wave field data and the reference beam by the spatial light modulator, synthesizes an object wave field generated by reflecting the object beam by the subject and the spherical wave field with each other so that an interference pattern is formed on the image sensor face, captures the interference pattern formed on the image sensor face, and then performs Fourier transformation to calculate an object wave field formed on the focal plane of the subject.

A device in holographic imaging comprises: at least two light sources, wherein each of the at least two light sources is arranged to output light of a unique wavelength; and at least one holographic optical element, wherein the at least two light sources and the at least one holographic optical element are arranged in relation to each other such that light from the at least two light sources incident on the at least one holographic optical element interacts with the at least one holographic optical element to form wavefronts of similar shape for light from the different light sources.

Among other things, a method comprises imaging a sample displaced between a sensor surface and a surface of a microscopy sample chamber to produce an image of at least a part of the sample. The image is produced using lensless optical microscopy, and the sample contains at least blood from a subject. The method also comprises automatically differentiating cells of different types in the image, generating a count of one or more cell types based on the automatic differentiation, and deriving a radiation dose the subject has absorbed based on the count.

Provided is a holographic microscope including an input optical system configured to emit polarized input beam, a first beam splitter configured to emit an object beam by reflecting a portion of the polarized input beam, and emit a reference beam by transmitting a remaining portion of the polarized input beam, a reference optical system configured to separate the reference beam into a first reference beam and a second reference beam, a camera configured to receive the first reference beam and the second reference beam and the object beam that is reflected by an inspection object, the camera including a micro polarizer array, wherein a first polarization axis of the first reference beam is perpendicular to a second polarization axis of the second reference beam.

A method of obtaining a focus term by using a periodicity of the focus term is provided. The focus term may be used in a plurality of operation processes for processing image data.

Motility contrast imaging (MCI) is a depth-resolved holographic technique to extract cellular and subcellular motion inside tissue. The holographic basis of the measurement technique makes it highly susceptible to mechanical motion. The motility contrast application, in particular, preferably includes increased mechanical stability because the signal is based on time-varying changes caused by cellular motion, not to be confused with mechanical motion of the system. The use of the resulting spectrogram response signatures, or fingerprint data, of known compounds is disclosed to screen new compounds for leads as to those having potentially beneficial mechanisms of action. The fingerprint data of known toxic compounds can be used to screen new compounds for toxicity.