An ultra high-resolution near infrared brain imager system includes a modular cap housing closely spaced multiple vertical-cavity surface-emitting lasersingle-photon avalanche photodiode array (VCSEL-SPAD) modules, each one of the VCSEL-SPAD modules including a linear VCSEL array and a SPAD detector.

Techniques to improve image quality in holography utilizing lenses made from materials with non-quantized anisotropic electromagnetic properties, such as birefringent materials, to advantageously split an incoming beam of light into two coincident beams with different focal lengths that interfere with one another and thus create holograms free of electro-optical or pixelated devices are disclosed for microscopy and other applications. The use of thin birefringent lenses and single crystal alpha-BBO lenses are introduced. Corresponding systems, methods and apparatuses are described.

A device including a light source, an image sensor, and a holder defining two positions between the light source and the image sensor. Each position is able to receive an object with a view to its observation. An optical system is placed between the two positions. Thus, when an object is placed in a first position, it may be observed, through the optical system, via a conventional microscopy modality. When an object is placed in the second position, it may be observed via a second lensless imagery modality.

The present invention is a cell observation device in which two-dimensional distributions of phase information and intensity information are computed based on hologram data obtained with a holographic microscope. An image display screen (100) displayed on a display unit includes an image display area (120) having two image display frames (121, 122). A phase image and intensity image corresponding to the same observation range on a cell culture plate (12), on which the cells to be observed are cultured, are displayed in the image display frames (121, 122), respectively. The wells on the plate, which are almost invisible on the phase image, are clearly visible on the intensity image. Conversely, the biological cells, which are almost invisible on the intensity image, are observable on the phase image. An observer specifies the range to be observed within a well on the intensity image, and subsequently enlarges the image corresponding to that range so as to observe the cells in detail on the phase image. Thus, the cells which are present within a desired range in the well can be assuredly observed.

A digital holographic microscope in which two digital holographic microscopes for detecting a fluorescence image and a phase image, respectively, are combined to be able to three-dimensionally measure a fluorescence image and a phase image at the same time, and perform measurement at a high SN ratio in all the polarization states including random light polarization. A first holographic optical system that, by using laser light, acquires a phase three-dimensional image due to interference light generated by superimposing object light which passes through a sample stage and reference light which does not pass through the sample stage onto each other. A second holographic optical system that, by using fluorescent excitation light, acquires a fluorescence three-dimensional image due to a fluorescence signal light, wherein phase measurement by the first holographic optical system and fluorescence measurement by the second holographic optical system are performed at the same time.

Systems and methods for simultaneous multi-channel off-axis holography are described. Multi-channel imaging systems can include a light system including a plurality of light sources configured to generate illumination and reference beams at a plurality of wavelengths, an illumination system configured to illuminate a target object with the illumination beams, an optical assembly configured to receive a reflected target beam and condition the target beam for recording at an optical imaging system, and a reference system configured to propagate the reference beams to the optical imaging system. The reference beams are interfered with the target beam at the optical imaging system to create interference patterns, which can be recorded in a collective image having a plurality of side lobes. Holographic information in the side lobes can be combined to generate 3D images having a substantially reduced signal to noise ratio.

Example apparatuses and methods relating to imaging systems are provided. An example imaging system may include an optical source configured to generate an optical beam, a beam splitter configured to split the optical beam into a reference beam and an object beam, and a beam combiner configured to route a combined beam with reference beam and object beam components along a common path into a target medium. In this regard, the target medium may act upon the combined beam to form a common path interference beam. The example imaging system may further include an imaging sensor configured to receive the common path interference beam and generate common path interference beam data associated with the common path interference beam, and an image data processor configured to analyze the common path interference beam data to generate image data describing the target medium.

An image display system is provided. The image display system includes: at least one holographic image acquiring device each configured to obtain holographic image information of a scene; an image synthesis device configured to generate holographic image synthesis information based on at least a part of the holographic image information obtained by the at least one holographic image acquiring device; and an image reconstruction device configured to reconstruct a holographic synthetic image in accordance with the holographic image synthesis information. The image display system can synthesize holographic image information to combine several holographic three-dimensional display scenes into one holographic three-dimensional scene, so that the user can perceive display effects almost the same as the real scenes, improving user experience. An image display method is also provided.

Provided is a digital holographic imaging apparatus, comprising: an illumination portion (10) having an illumination light emission surface (32i) for emitting coherent light of a specific wavelength as illumination light toward an object (1) side relative to the illumination light emission surface (32i), and a reference light emission surface (32r) for emitting the coherent light, as reference light, in a direction opposite to the illumination light; and an image sensor (50) located on the reference light emission surface (32r) side of the illumination portion (10) and imaging an interference pattern between object light having been modulated by the object (1) and passed through the illumination portion (10) and the reference light of the illumination light, the image sensor (50) having a pixel array (51) comprising two-dimensionally aligned pixels.

A time delay error occurring in the case of acquiring two holograms (object hologram and reference hologram) necessary for reconstruction in the related art or in the case of acquiring four physical holograms having different phase shift degrees may be removed. DC noise (including background noise) may be completely removed by using a software-implemented phase shifting method.