A manufacturing method of an optical element applied to a miniature microscope includes the steps of: emitting a signal light and a reference light to an optical material; and forming a plurality of gratings on the optical material by interfering the signal light and the reference light. A miniature microscope is also disclosed.

Briefly stated, technologies are generally described for providing a computer-generated holography (CGH). Example devices/systems described herein may use one or more of a server device and/or a client device. The server device may be configured to provide CGH data to a client device including a holographic image display unit. The server device may receive information on the holographic image display unit from the client device, calculate the CGH data from three-dimensional image data and the information on the holographic image display unit, and/or transmit the CGH data to the client device. The client device may be configured to provide a holographic image. The client device may reconstruct the holographic image on the holographic image display unit using CGH data and a reconstruction beam, transmit information on the holographic image display unit to the server device, and/or receive the CGH data from the server device.

A microscope, method and computer program for obtaining quantitative phase images by digital holographic microscopy. The microscope includes: a coherent light source (1) and a beam splitter (3) for generating an object beam (Lo) for illuminating a sample, and a reference beam (Lr); an optical system with a main optical path making up a telecentric afocal system, and a reference optical path; and recording means (12) recording a hologram of said sample in the image plane of the optical system. The method includes recording a hologram in the image plane of an optical telecentric afocal system. The computer program is adapted for implementing part of the steps of the method.

Embodiments described herein relate to lens-free imaging. One example embodiment may include a lens-free imaging device for imaging a moving sample. The lens-free imaging device may include a radiation source configured to emit a set of at least two different wavelengths towards the moving sample. The lens-free imaging device is configured to image samples for which a spectral response does not substantially vary for a set of at least two different wavelengths. The lens-free imaging device may also include a line scanner configured to obtain a line scan per wavelength emitted by the radiation source and reflected by, scattered by, or transmitted through the moving sample. The line scanner is configured to regularly obtain a line scan per wavelength. Either the radiation source or the line scanner is configured to isolate data of the at least two different wavelengths.

A new optical arrangement that creates high efficiency, high quality Fresnel Incoherent Correlation Holography (FINCH) holograms using transmission liquid crystal GRIN (TLCGRIN) diffractive lenses has been invented. This is in contrast to the universal practice in the field of using a reflective spatial light modulator (SLM) to separate sample and reference beams. Polarization sensitive TLCGRIN lenses enable a straight optical path, have 95% transmission efficiency, are analog devices without pixels and are free of many limitations of reflective SLM devices. An additional advantage is that they create an incoherent holographic system that is achromatic over a wide bandwidth. Two spherical beams created by the combination of a glass and a polarization sensitive TLCGRIN lenses interfere and a hologram is recorded by a digital camera. FINCH configurations which increase signal to noise ratios and imaging speed are also described.

Systems and methods for sub-aperture correlation based wavefront measurement in a thick sample and correction as a post processing technique for interferometric imaging to achieve near diffraction limited resolution are described. Theory, simulation and experimental results are presented for the case of full field interference microscopy. The inventive technique can be applied to any coherent interferometric imaging technique and does not require knowledge of any system parameters. In one embodiment of the present application, a fast and simple way to correct for defocus aberration is described. A variety of applications for the method are presented.

Disclosed is a coherence-adjustable digital holography system. More particularly, the coherence-adjustable digital holography system includes a light source part for generating low-interference light; a dispersion part for dispersing the generated light, an adjustment part for adjusting coherence by adjusting a spectrum bandwidth of the light which has passed through the dispersion part; and a detection part for detecting a holographic image of a subject from the adjusted light. In accordance with such a configuration, an interference fringe may be easily obtained through coherence adjustment, whereby the accuracy of a detected holographic image may be improved.

An observation apparatus includes a light source, a beam splitter, a mirror, a mirror, a cylindrical lens, a lens, a cylindrical lens, a beam splitter, a lens, a frequency shifter, an imaging unit, and an analysis unit. The analysis unit generates a complex amplitude image of each of a plurality of light irradiation directions of object light based on time series data of an interference intensity image output from the imaging unit, and generates a three-dimensional complex differential interference image of an observation object based on the complex amplitude image of each of the plurality of light irradiation directions. The analysis unit obtains a three-dimensional phase image of the observation object based on the three-dimensional complex differential interference image.

A device and method for measuring a surface of an object, including at least one light source, at least one optical sensor, and an interferometry device having a measurement arm and a reference arm, the former directing light from each light source towards the surface of the object and directing light from the surface towards each optical sensor; the measurement device, in an interferometry configuration, illuminating the reference arm and the measurement arm with each light source and directing the light from the measurement arm and the reference arm towards each optical sensor to form an interference signal; the measurement device, in an imaging configuration illuminating at least the measurement arm and directing the light from the measurement arm towards the optical sensor to form an image of the surface; the measurement device including a digital processor producing, from the interference signal and the image, information on the surface.

In various embodiments a module for generating an interference pattern for producing a digital holographic image is provided. The module comprises an adaptive lens arrangement configured to receive, from a microscope, an object wave of an intermediate image of a sample to be examined, and to generate an adapted object wave of the intermediate image of the sample by reducing a curvature of the object wave of the intermediate image; a reference input interface configured to receive an optical fiber delivering a reference wave from the coherent light source to the module and an interference arrangement configured to generate an interference pattern to be received by an imaging sensor arrangement, wherein the interference pattern is based on the adapted object wave and the reference wave from a coherent light source; wherein a position of the reference input interface of the module is configured to be adjustable with respect to at least two directions (x-y), wherein at least one of the adjustable directions is in parallel to a propagation direction of the reference wave leaving the optical fiber.