Example embodiments relate to imaging devices for in-line holographic imaging of objects. One embodiment includes an imaging device for in-line holographic imaging of an object. The imaging device includes a set of light sources configured to output light in confined illumination cones. The imaging device also includes an image sensor that includes a set of light-detecting elements. The set of light sources are configured to output light such that the confined illumination cones are arranged side-by-side and illuminate a specific part of the object. The image sensor is arranged such that the light-detecting elements detect a plurality of interference patterns. Each interference pattern is formed by diffracted light from the object originating from a single light source and undiffracted light from the same single light source. At least a subset of the set of light-detecting elements is arranged to detect light relating to not more than one interference pattern.

The present invention relates to a method of detecting a possible infection of malaria in a patient using a digital optical microscope

A holographic imaging device and method realizes both a transmission type and a reflection type, and also realizes a long working distance wide field of view or ultra-high resolution. Object light emitted from an object, sequentially illuminated with parallel illumination light whose incident direction is changed, is recorded on a plurality of object light holograms for each incident direction using off-axis spherical wave reference light. The reference light is recorded on a reference light hologram using in-line spherical wave reference light being in-line with the object light. An object light wave hologram and its spatial frequency spectrum at the object position are generated for each incident direction using each hologram. A synthetic spectrum which occupies a wider frequency space is generated by matching each spectrum in the overlapping area, and a synthetic object light wave hologram with increased numerical aperture is obtained thereby.

The present disclosure relates to devices and methods configured to perform drug screening on cells. At least one embodiment relates to a lens-free device for performing drug screening on cells. The lens-free device includes a substrate having a surface. The lens-free device also includes a light source positioned to illuminate the cells, when present, on the substrate surface with a light wave. The lens-free device further includes a sensor positioned to detect an optical signal caused by illuminating the cells. The substrate surface includes a microelectrode array for sensing an electrophysiological signal from the cells.

The present disclosure relates to improved holographic reconstruction device and a method. In one aspect, the present disclosure relates to improved holographic reconstruction device and method that can measure a digital hologram regardless of optical characteristics of an object to be measured, by an all-in-one type system integrating a transmissive system that measures an object transmitting light and a reflective system that measures an object reflecting light.

An optical detection device includes a light source emitting a light beam whose electromagnetic field, a means adapted to divide the beam into a first beam defining a first reference pathway and a second beam defining a second sample pathway, a modulation system frequency-shifting the electromagnetic fields of the two beams, a beam coupler adapted to collect the beams, an optical detection system adapted to detect the signal arising from the interference between the beams and coupled via the coupler, the sample being placed in the sample pathway, the optical detection system comprising an optical detector and a device adapted to measure the amplitude and the phase of the signal, an opaque screen comprising an optical aperture is placed at the level of a zone of a sample, in proximity to the sample, in the sample pathway.

A method for observing a sample (10), the sample lying in a plane of the sample defining radial coordinates, the method comprising the following steps: a) illuminating the sample using a light source (11), able to emit an incident light wave (12) that propagates toward the sample along a propagation axis (Z); b) acquiring, using an image sensor (16), an image (I.sub.0) of the sample (10), said image being formed in a detection plane (P.sub.0), the sample being placed between the light source (11) and the image sensor (16), such that the incident light wave sees an optical path difference, parallel to the propagation axis (Z), by passing through the sample; c) processing the image acquired by the image sensor;
wherein the processing of the acquired image comprises taking into account vectors of parameters, respectively defined at a plurality of radial coordinates, in the plane of the sample, each vector of parameters being associated with one radial coordinate, and comprising a term representative of an optical parameter of the sample, at least one optical parameter being an optical path difference induced by the sample at the radial coordinate, the vectors of parameters describing the sample.

A method for determining a parameter of a particle present in a sample, the method comprising the following steps: a) illuminating the sample with the light source, the light source emitting an incident light wave that propagates along a propagation axis; b) acquiring an image of the sample with the image sensor, the image sensor being exposed to an exposure light wave; c) determining a position of the particle in the detection plane; d) on the basis of the acquired image, applying a propagation operator, for a plurality of distances from a detection plane, so as to estimate, at each distance, a complex amplitude of the exposure light wave; e) on the basis of the complex amplitude estimated, at various distances, obtaining a profile representing a variation of the complex amplitude of the exposure light wave along an axis parallel to the propagation axis and passing through the position of the particle.

The particle may associated with a set of parameters, comprising at least a size of the particle and a refractive index of the particle.

The invention relates to a digital holographic microscope, DHM. The DHM comprises a coherent light source (401, 501) for illuminating a sample in a sample holder in a first image plane (101,201,407, 507). The DHM further comprises a detector, e.g. a camera (412, 512), arranged to record images of the sample in the sample holder. The DHM further comprises means for dividing the base light beam into different portions and causing the different portions of the light beam to interfere with each other at the detector and a light beam guiding system for guiding a light beam to the sample and the detector. The DHM further comprises a light reducing arrangement for reducing the intensity of the light in the light beam directed to the sample. The light reducing arrangement includes first lens (102) for collimating the light in the first divided beam scattered by a particle (106) comprised in the sample and a spatial filter (108, 206, 413) arranged at or in the vicinity of the focal plane (103, 203) of said first lens (102) in order to reduce the intensity of the focused light passing through the sample located in the first image plane (101, 201, 307, 407). By this arrangement, the majority of unscattered light passing through the sample is filtered off and the majority of the light scattered by a particle (106) in the sample is guided via a light guiding system to the detector.

A method for observing a sample includes illuminating the sample with a light source and forming a plurality of images, by an imager, the images representing the light transmitted by the sample in different spectral bands. From each image, a complex amplitude representative of the light wave transmitted by the sample is determined in a determined spectral band. The method further includes backpropagation of each complex amplitude in a plane passing through the sample, determining a weighting function from the back-propagated complex amplitudes, propagating the weighting function in a plane along which the matrix photodetector extends, updating each complex amplitude, in the plane of the sample, according to the weighting function propagated.