A device and a method for observing an object by imaging, or by lensless imaging. The object is retained by a holder defining an object plane inserted between a light source and an image sensor, with no enlargement optics being placed between the object and the image sensor. An optical system is arranged between the light source and the holder and is configured to form a convergent incident wave from a light wave emitted by the light source, and for forming a secondary light source, conjugated with the light source, positioned in a half-space defined by the object plane and including the image sensor, such that the secondary source is closer to the image sensor than to the holder. This results in an image with a transversal enlargement factor having an absolute value of less than 1.

A holographic image recording method is disclosed, that is realized through utilizing a holographic image fetching and recording device, including the following steps: using an image fetching device to fetch an image of a target object placed on a rotation table rotating at a fixed speed, the image thus obtained is transmitted to a display panel through a connection line; using a light emitting unit to emit coherent light to a first reflector; that reflects the coherent light to light splitter; and the light splitter splits the coherent light along a first light path and a second light path into an object light and a reference light, and transmits them onto a holographic film to interfere with each other, to form a holographic image. A holographic image reconstructing method is also disclosed, to reconstruct and form a 3D holographic image floating above the holographic film.

Provided are a morphological cell parameter-based erythrocyte test method and digital holographic microscope used therein, and the morphological cell parameter-based erythrocyte test method includes performing modeling to create a 3D image of an erythrocyte to be tested and measuring morphological parameters of the erythrocyte based on the 3D image. The morphological cell parameter-based erythrocyte test method performs modeling of a 3D image for an erythrocyte to be tested and measures morphological parameters of the erythrocyte based on the 3D image. Therefore, time and effort consumed in measurement may be reduced, and accuracy of the measurement is excellent.

A method for digital holographic microtomography comprises (a) providing at least one wavefront controlling device for driving a sample to be rotated and/or an incident beam scanning the sample, (b) utilizing a digital holographic access unit for recording the transmitted or reflected wavefronts of the sample, (c) utilizing a digital holography reconstructing method for reconstructing the transmitted or reflected wavefronts of the sample, and (d) utilizing a tomographic reconstruction approach for reconstructing three dimensional image information of the sample.

Methods and apparatus are configured for focusing and imaging of translucent materials with decreased size and complexity and improve resolution. The methods and apparatus provide improved focusing and imaging with decreased size and weight, so as to allow use in many fields.

A magnetic field controlled guidestar for focusing light deep inside scattering media using optical phase conjugation. Compared with the optical and ultrasonic field, the magnetic field has an exceptional penetration depth. The magnetic particle guidestar has a high light-tagging efficiency, good biocompatibility, and a small diameter which enables a sharp and bright focusing deep inside biological tissue. This new method can benefit a wide range of biomedical applications including deep-tissue imaging, neural modulation, and targeted photothermal and photodynamic therapies.

An optical detection device includes a light source emitting a light beam whose electromagnetic field, a means adapted to divide the beam into a first beam defining a first reference pathway and a second beam defining a second sample pathway, a modulation system frequency-shifting the electromagnetic fields of the two beams, a beam coupler adapted to collect the beams, an optical detection system adapted to detect the signal arising from the interference between the beams and coupled via the coupler, the sample being placed in the sample pathway, the optical detection system comprising an optical detector and a device adapted to measure the amplitude and the phase of the signal, an opaque screen comprising an optical aperture is placed at the level of a zone of a sample, in proximity to the sample, in the sample pathway.

The present invention can realize both a transmission type and a reflection type, and provides a holographic microscope which can exceed the resolution of the conventional optical microscope, a hologram data acquisition method for a high-resolution image, and a high-resolution hologram image reconstruction method. In-line spherical wave reference light (L) is recorded in a hologram (I.sub.LR) using spherical wave reference light (R), and an object light (O.sup.j) and an illumination light (Q.sup.j) are recorded in a hologram (I.sup.j.sub.OQR) using a spherical wave reference light (R) by illuminating the object with an illumination light (Q.sup.j, j=1, . . . , N) which is changed its incident direction. From those holograms, a hologram (J.sup.j.sub.OQL), from which the component of the reference light (R) is removed, is generated, and from the hologram, a light wave (h.sup.j) is generated. A light wave (c.sup.j) of the illumination light (Q.sup.j) is separated from the light wave (h.sup.j), and using its phase component (.sup.j=c.sup.j/|c.sup.j|), a phase adjustment reconstruction light wave is derived and added up as (H.sub.P=h.sup.j/.sup.j), and an object image (S.sub.P=|H.sub.P|.sup.2) is reconstructed.

The invention relates to a method for observing a sample, in particular an anatomopathological slide formed from a thin thickness of a sampled biological tissue. It includes a step of illuminating the sample with a light source and acquiring, with an image sensor, an image representing the light transmitted by the sample. The image undergoes holographic reconstruction, so as to obtain a representation, in the plane of the sample, of the light wave transmitted by the latter. The method includes applying an impregnating fluid to the sample, such that the sample is impregnated with said impregnating liquid, said impregnating liquid having a refractive index strictly higher than 1.

A method for digital holographic microtomography comprises (a) providing at least one wavefront controlling device for driving a sample to be rotated and/or an incident beam scanning the sample, (b) utilizing a digital holographic access unit for recording the transmitted or reflected wavefronts of the sample, (c) utilizing a digital holography reconstructing method for reconstructing the transmitted or reflected wavefronts of the sample, and (d) utilizing a tomographic reconstruction approach for reconstructing three dimensional image information of the sample.